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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Aminoacyl tRNA synthetase"”.
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Modelling neuronal mitochondrial aminoacyl-tRNA synthetase defects
… and skeletal muscle. Mutations in mitochondrial aminoacyl-tRNA synthetase (MT-ARS) genes, which are crucial for mitochondrial protein synthesis and energy production via oxidative phosphorylation, are implicated in a variety of severe neurological and multisystemic diseases. Among these, …
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Functional analysis of a class II aminoacyl-tRNA synthetase
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1994.
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Experimental and Computational Dynamics of an Aminoacyl-tRNA Synthetase System
… is dependent upon correct recognition and aminoacylation of transfer RNA (tRNA) by their cognate aminoacyl-tRNA synthetases (AARSs). Methionine is the amino acid that acts as the start codon for every protein and can also be found within a protein message. Methionyl-tRNA synthetase (MetRS) …
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NMR studies of RNA binding domains of human lysyl aminoacyl tRNA synthetase
<p>Human lysyl aminoacyl tRNA synthetase (hLysRS) is a multi-functional aminoacyl tRNA synthetase which is primarily involved in protein biosynthesis as well as crucial processes ranging from proinflammatory response to signal transduction. One important, non-canonical function of hLysRS is to …
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Characterizing the landscape of aminoacyl-tRNA synthetase protein production in Bacillus subtilis
… all cases. Here I use a model enzyme family, the aminoacyl tRNA synthetases (aaRS), to explore how sensitive Bacillus subtilis are to changes in aaRS production from the molecular to phenotypic level. This culmination of protein levels, functional output, and fitness, leads to a complete "fitness …
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Identification and Evolution of New Orthogonal Aminoacyl-tRNA Synthetase/tRNA Pairs for Genetic Code Expansion
… amino acids is the scalable discovery of aminoacyl-tRNA synthetase (aaRS)–tRNA pairs (the components of the cellular translational machinery which specify the matching between codons and amino acids) that are orthogonal in their aminoacylation specificity. An orthogonal pair is composed of …
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Investigating and expanding the functionality of RNA catalysts: Studies of the hepatitis delta virus, the hammerhead, and the aminoacyl-tRNA synthetase-like ribozymes
… ribozyme that mimics the function of the natural aminoacyl-tRNA synthetase. The design was based on the sequence of a naturally occurring riboswitch and an in vitro selected ribozyme that could charge tRNA with chemically synthesized unnatural amino acid substrates. The engineered ribozyme …
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From the Origins of Life to Epidemics: Bayesian Inference, Stochastic Simulation, and Dynamics of Bioinformatic Systems
… method for phylogenetic inference on the aminoacyl-tRNA synthetase enzymes, which are responsible for attaching amino acids to tRNA molecules and are thereby instrumental to the functioning of the genetic code, and (c) the development, testing, and implementation of, and results generated …
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Expanding the genetic code of a mouse
… acids into proteins(Greiss and Chin, 2011). An aminoacyl-tRNA synthetase and a tRNA are used to specifically insert the unnatural amino acid during mRNA translation, in response to an amber stop codon (UAG) placed at a user-defined site in a gene interest (Davis and Chin, 2012) In this study, I …
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Aminoacylation kinetics and specificity for viral genomic RNAs
The esterification of amino acids to the tRNA-like structures of viral genomic RNA was studied as catalyzed by amino acyl tRNA synthetases from E. coli, yeast, bean and rat. Peanut stunt virus RNA was esterified with tyrosine in the presence of bean tyrosyl-tRNA synthetase to a maximum of 22 mole …
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Investigation of the mechanism of radical propagation in E. coli ribonucleotide reductase by site-specific incorporation of unnatural amino acids
… expressed protein ligation and suppressor tRNA/aminoacyl-tRNA synthetase (RS) methodologies to site-specifically insert unnatural tyrosine analogues into 12 and a2, at residues believed to be involved. On the basis of results with the radical traps 3,4-dihydroxyphenylalanine (DOPA) and …
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Orthogonal pair-directed codon reassignment as a tool for evaluating the factors affecting translation in E. coli
… have focused on using an engineered orthogonal aminoacyl-tRNA and aminoacyl tRNA-synthetase pairs (tRNA/aaRS) to direct incorporation of ncAAs at amber stop codons. In order to further expand the genetic code to 22 or more amino acids, additional codons must be targeted for reassignment to …
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Rational Identification of Neuropilin-1 Inhibitors: From Deep-Learning to Drug Repurposing
… between Charcot–Marie–Tooth–associated aminoacyl tRNA synthetase mutants and the NRP1-b1 pocket were modelled to extract short binding segments. In parallel, small molecule inhibition was pursued through structure-based virtual screening of an FDA-approved library using focussed and …
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Genetic Code Expansion in Mammalian Cells
… the translational machinery often importing an aminoacyl-tRNA synthetase (aaRS)/tRNA pair that does not cross-react with the endogenous aaRS/tRNA pairs. This approach has allowed for co-translational incorporation of a variety of non-canonical amino acids, including amino acids for …
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Computational studies of origins of life scenarios
… a modern biochemical reaction, the charging of tRNA by an aminoacyl-tRNA synthetase, in order to shed light on the possible chemistry one may want to consider in problems relating to the origins of life. The question of how codons came to be associated with specific amino acids in the present …
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Exogenous leucyl-tRNA synthetase inhibits mouse skeletal muscle differentiation
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-05-01
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Characterization of the Operational Trna Code for Amino Acids Through Supervised Machine Learning
The operational tRNA code for amino acids is defined as a set of sequence- or structure-dependent rules governing the aminoacylation of tRNAs outside and independent of the anticodon. Previous efforts to uncover these rules have included experimental studies using synthetic and chemically modified …
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Simulation and visualization of dynamics in RNA-protein complexes in translation
… the molecular instantiation of the genetic code, tRNA plays a central role in the translational machinery where it interacts with several proteins and other RNAs during the course of protein synthesis. We use molecular dynamics (MD) simulations informed by evolutionary analysis to investigate the …
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