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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 67 for “"Amino acid substitution"”.

  1. Facile protein and amino acid substitution reactions and their characterization using thermal, mechanical and optical techniques

    Budhavaram, Naresh KumarVirginia Tech2010

    … The first objective was to quantify protein and amino acid substitution reactions. Michael addition reactions were used to modify the amino acids and protein. Amino acids alanine, cysteine, and lysine, and protein ovalbumin (OA) were substituted with different concentrations of ethyl vinyl …

    vt Repository record

  2. Navigating Molecular Evolution Using Substitution Mappings

    Low, AndrewCarleton University2017

    Since first proposed in 2002, Bayesian substitution mappings have found less use than might have been expected in the field of molecular evolution. Here, I first create a sequence simulator capable of generating true substitution mappings for simulated data under any time-reversible model in order …

    carleton Repository record

  3. A clinical and molecular analysis of Clostridium difficile strains isolated from Groote Schuur Hospital

    Brock, Tunehafo ElisabethDepartment of Molecular and Cell Biology2015

    … be lacking clinical sensitivity, while nucleic acid amplification tests (Hain Lifescience CDiff and Cepheid GeneXpert) were far more sensitive in the local clinical setting. PCR ribotyping identified all the A-B+ strains as PCR ribotype 017, which was the prevalent strain type (47%). Genotyping …

    cape-town Repository record

  4. Estimation and Comparison of HIV-Specific Substitution Matrices

    Kee, Jia Jin2014

    Amino acid substitution matrices are commonly used for sequence alignment, phylogenetic inference and sequence comparison. Empirical organism-specific substitution matrices constructed using only sequence data from a particular organism are thought to lead to more accurate analyses. In HIV …

    washington Repository record

  5. Glycoprotein mimetic materials - synthetic methods and study of viral inhibition properties

    Seifried, Brian(Brian Michael)Massachusetts Institute of Technology2019

    … while the second uses combination of global amino acid substitution and copper()-catalyzed alkyne-azide cycloaddition (CuAAC). The tyrosine enrichment required to prepare elastin-like-polypeptides (ELP) proteins for diazo coupling reactions prevented protein expression at 96 pentapeptide …

    mit Repository record

  6. The Mechanism of Activation of Potassium Dependent and Potassium Independent Asparaginases from Common Bean (Phaseolus vulgaris)

    Ajewole, Ebenezer OluwafemiThe University of Western Ontario2016

    … critical role in alkali metal coordination. This amino acid residue is replaced by an isoleucine in PvAspG-T2. Reciprocal mutants of the enzymes were produced and the effect of the amino acid substitution on the kinetic parameters, secondary structure conformation, allosteric effector binding and …

    uwo Repository record

  7. Dissecting the Multifaceted Relationship Between Maize and Cochliobolus carbonum race 1

    Chu, Kevin

    … The HM1A protein differs from HM1 by five amino acid substitutions while HM1-SM1 and HM1-SM2 have a single amino acid substitution each in the predicted NADPH binding pocket. Given that gene and protein expression of these APR alleles do not increase with age, the APR phenotype must be …

    purdue-thes Repository record

  8. Sequence Analysis of PMEL17 as Candidate Gene for Causing Rat-Tail Syndrome in Cattle

    Hecht, Benjamin C.2006

    … polymorphism (SNP) at codon 612 resulting in an amino acid substitution (A?E). The 3-bp deletion in exon 1 of Pmel17 is in 100% concordance with the rat-tail phenotype in this research population and may be causative of the rat-tail phenotype.

    byu Repository record

  9. Molecular characterization of ovine GM1 gangliosidosis

    Walker, KristenUniversity of Illinois at Urbana-Champaign2013

    … cysteine to phenylalanine (Cys229Phe) amino acid substitution. A PCR-RFLP assay using AciI restriction enzyme was designed for genotyping lambs. To date, 1664 animals have been genotyped using the DNA-based diagnostic and all affected lambs were homozygous for this mutation. Conversely, …

    uiuc Repository record

  10. Citrulline metabolism in cultured fibroblasts : citrullinemia analysis and nitric oxide production

    Shires, Karen LesleyDivision of Chemical Pathology1994

    … This mutation resulted in a glycine -> serine amino acid substitution at position 324 in the ASS subunit protein sequence. Although this glycine residue was not found to occur in any potential substrate binding sites, it was shown to be highly conserved among species, indicating a possible role …

    cape-town Repository record

  11. Characterization of mutant N², N²-dimethylguanosine-specific tRNA methyltransferases from Saccharomyces cerevisiae

    Reuben, Melanie1997

    … the transfer of two methyl groups to the 2-amino group of guanosine at position 26 in tRNA. Neither the tertiary structure nor the location of the active sites are known for m$\sbsp{2}{2}$Gtase. In an attempt to characterize this enzyme, the trm1 gene from the Saccharomyces cerevisiae strain …

    concordia Repository record

  12. The Path to an Orally Administered Protein Therapeutic for the Treatment of Diabetes Mellitus

    James, Susan Clardy

    … attachment of B<sub>12</sub> at the B1 vs. B29 amino acid position. The insulinotropic profile of both conjugates was evaluated in streptozotocin induced diabetic rats. Oral administration of the conjugates produced significant drops in blood glucose levels, compared to an orally administered …

    syracuse-diss Repository record

  13. Characterization of HIV-1 Rev mutants

    Chang, Jennifer LynnWright State University2012

    … proteins owing to the presence of a shared amino acid sequence. Kin13 proteins are potent MT depolymerizing agents affecting MT behavior during mitosis.To test this hypothesis, point mutations were introduced into Rev substituting amino acids shared with Kin13. In this study, eight mutant …

    ohiolink Repository record

  14. Creation of Bioinformatics Approaches to Understand Host-Pathogen Molecular Mimicry

    Rich, Kaylee DawnGraduate Studies2024

    … of five protein search tools with two amino acid substitution scoring matrices on varying query lengths. From these results, I determined the combination best suited to mimicry detection and implemented a new filtering method which better assesses similarity between pathogen and host …

    calgary Repository record

  15. The SEC9 gene is essential for exocytosis in yeast and encodes a dispensable amino terminal domain

    Champion, Kathleen M.University of Illinois at Urbana-Champaign2011

    … appears to be composed of two domains: an amino terminal domain that is dispensable, and a carboxy terminal domain that is predicted to form an amphiphilic helix. The carboxy terminal one-third of the putative SEC9$\sb{\rm p}$ is necessary and sufficient to complement sec9-4$\sp{ts}$ and …

    uiuc Repository record

  16. The Influence of Structural Constraints on Protein Evolution

    Perron, UmbertoUniversity of Cambridge2020

    … my PhD project was to create astructurally aware amino acid substitution model in which proteins are represented usingan expanded alphabet that relays both amino acid identity and structural information.Each character in this alphabet specifies an amino acid as well as information aboutthe rotamer …

    cambridge Repository record

  17. Mechanisms of attenuation of the live Junin virus vaccine strain, Candid #1

    Manning, John TylerThe University of Texas Medical Branch at Galveston2018

    … attenuation of Candid #1 to a smaller degree. An amino acid substitution within the RING domain of the Candid #1 Z protein results in the protein budding from the cell independently, and there is a significant decrease in interaction between GP and Z in a plasmid expression system. Together, these …

    utmb Repository record

  18. Expression and Function of the Chloroplast-encoded Gene matK

    Barthet, Michelle MarieVirginia Tech2006

    … evolving gene at nucleotide and corresponding amino acid levels. The high number of nucleotide substitutions and length mutations in matK has provided a strong phylogenetic signal for resolving plant phylogenies at various taxonomic levels. However, these same features have raised questions as …

    vt Repository record

  19. Regional whole plant and molecular response of Kochia scoparia to glyphosate

    Wiersma, Andrew, author, Westra, Philip, advisor, Leach, Jan, advisor, Reddy, Anireddy, committee member, Holtzer, Thomas, committee memberColorado State University. Libraries2012

    … of the EPSPS binding site proline confirmed that amino acid substitution at that residue was not responsible for resistance in K. scoparia. However, quantitative PCR estimates of EPSPS copy number revealed increased copy number in all glyphosate-resistant individuals —ranging from 3 to 9 EPSPS …

    colostate Repository record

  20. Calcium Triggered Synaptic Vesicle Exocytosis

    Pang, Zhiping2010

    … Using two knock-in mouse lines each with single amino-acid substitution, namely D232N and D238N in synaptotagmin-1, combined with electrophysiology, I found evoked release in D232N mutant neuronal cultures is significantly increased, whereas in D238N cultures release is slightly but significantly …

    utswmed Repository record

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