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Showing 1 to 20 of 330 for “"Amino acid sequence"”.
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The amino acid sequence of chicken histone F3
… electrophoretic mobility, N- and C-terminal amino acid residues and amino acid analysis. The primary structure of this histone was established without resorting to the use of overlapping sequences. This has been achieved with specific chemical cleavages rather than enzymatic degradations …
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Amino Acid Sequence of Cytochrome C3 From Desulfovibrio Vulgaris
Made available in DSpace on 2014-12-11T18:16:50Z (GMT). No. of bitstreams: 1 7212413.pdf: 1916231 bytes, checksum: fd51efdadcd8cb3d4bcf0e3113a1cab1 (MD5) Previous issue date: 1971
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Structural Characterization of Donkey Lactoferrin: Amino Acid Sequence and Glycan Compositions
… characterization of donkey s lactoferrin sequence, that, at least for the covered sequence, differs from the mare s genomic deduced sequence (UniProtKB Acc. Nr. O77811) by five point substitutions located at positions 91 (Arg->His), 328 (Thr->Ile/Leu), 466 (Ala->Gly), 642 (Asn->Ser) and …
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Amino Acid Sequence Studies on the Beta Subunit of Bacterial Luciferase
Peptides comprising 80% of the amino acid sequence of the (beta) subunit of bacterial luciferase from Vibrio harveyi were isolated and their amino acid sequences were determined by Edman degradation. The protein was fragmented by digestion with S. aureus V8 protease, trypsin, trypsin after …
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The amino acid sequence of the major alpha globin of the rat (Wistar, Rattus norvegicus)
… sodium ions in the presence of 8 M urea. 2. The amino acid sequence of the ∝ chain was substantially determined. The soluble tryptic peptides prepared from the native ∝ globin and from the aminoethylated ∝ globin were separated by peptide mapping. Sequencing of the tryptic peptides was carried …
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Protein Three -Dimensional Structure: Theoretical and Computational Aspects of Structure Prediction Based on the Amino Acid Sequence
… for ab initio structure prediction, we examined sequence-structure relationships in these clusters. It turns out that while structural characterization is nearly complete, the mapping from sequence to structure is not unique and no significant correlations exist between the sequences in a given …
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Thelin Operon in Pseudomonas Incognita: Cloning, Nucleotide Sequence and Deduced Amino Acid Sequence of Cytochrome P-450lin and Other Enzymes Involved in the Catabolism of Linalool
… P-450lin NH$\sb2$-terminal and tryptic-fragment amino acid sequence, the cytochrome P-450lin operon (lin) was cloned. Four overlapping clones representing 10.8 kb of novel sequence were isolated and sequenced. Eight open reading frames were found in this sequence. The GenBank, EMBL, and SwissProt …
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Analysis of protein secondary structure via the discrete wavelet transform
… the wavelet technique, we convert the primary amino acid sequence of the protein to a numerical signal using the hydrophobic tendencies associated with the amino acids. The data used in this project consists of both a + B and a/B proteins coming from the Structural Classification of …
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Protein structure recognition: from eigenvector analysis to structural threading method
… process. We found a strong correlation between amino acid sequence and the corresponding native structure of the protein. Some applications of this correlation were discussed in this dissertation include the domain partition and a new structural threading method as well as the performance of …
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Characterization of the large diameter fibers in egg case silk : identification of a core fibroin, TuSp1, and localization of fibroin-like molecules, ECPs, from the black widow spider, latrodectus hesperus
… At the time of these studies, fibroin eDNA sequences that encode egg case silk had not been reported in the literature. This study used conventional nucleic acid-nucleic acid screening of a eDNA library to isolate a novel gene, named tubuliform spidroin 1 , from the black widow spider …
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Molecular characterization of phosphoribulokinase and rubisco activase from Chlamydomonas reinhardtii
… coding region were isolated and sequenced. The Chlamydomonas and spinach mature proteins possessed 75% amino acid sequence identity and similar kinetic properties. The transit peptides possessed almost no amino acid sequence identity. Genomic DNA blot analysis suggested the …
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Studies of the UNC-45A Molecular Chaperone Expression in Human Cancer
… region (UTR) and 944 isoform had a specific 15 amino acid sequence close to its N-terminus. Furthermore, we showed that UNC-45A 929 accumulated higher level in human breast cells. More importantly, the ubiquitin-proteasome system degraded endogenous UNC-45A and the 15 amino acid sequence unique …
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Studies of the UNC-45A Molecular Chaperone Expression in Human Cancer
… region (UTR) and 944 isoform had a specific 15 amino acid sequence close to its N-terminus. Furthermore, we showed that UNC-45A 929 accumulated higher level in human breast cells. More importantly, the ubiquitin-proteasome system degraded endogenous UNC-45A and the 15 amino acid sequence unique …
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Cloning and regulation of rat and Xenopus laevis hepatic 3-hydroxy-3-methylglutaryl Coenzyme A reductase
… A (HMG-CoA) reductase have been cloned and sequenced. The rat liver HMG-CoA reductase cDNA clone contains one open reading frame coding for 887 amino acids. Its 106 nucleotide long 5$\sp\prime$-noncoding region contains no additional start codons. It does not contain a poly(A) tract at the …
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Isolation And Characterization Of Human Ribosomal Protein Complementary-dnas
… due to insufficient homology between the coding sequences of yeast ribosomal protein S10 and human ribosomal protein S6. In another set of cross hybridization experiments a rat ribosomal protein S11 cloned cDNA was used successfully to probe a human cDNA library and identify a full-length human …
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Studies of verticillium wilt and characterization of candidate verticillium wilt resistance genes in the mint species Mentha longifolia (L) Huds
… resistance genes. The mint RGAs shared predicted amino acid sequence similarity with disease resistance genes and RGAs from various other plant species, and were grouped into seven distinct families based on DNA and predicted amino acid sequence similarity. In addition to the NBS-related RGAs, a …
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A novel bioactive peptide from the defensive skin secretion of Phyllomedusa sauvagii
… and translated open-reading frame (ORF) amino acid sequence of this precursor-encoding cDNA. BLAST analysis indicated that the amino acid sequence of the novel peptide QUB-2909 (ALWKNMLKGIGKLAGQAALGAVKTLVGAE) belonged to the dermaseptin family. QUB-2909 was then synthesised by use of …
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Applications of mass spectrometry to protein characterization
… Using a strategy of homology comparison, the amino acid sequence was determined solely by mass spectrometric methods. The nature of aldose reductase oxidation was also identified.
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A Structural and Functional Analysis of the Human Angiotensin II Type-2 Receptor
… we used synthetic peptides derived from the amino acid sequence of the cytoplasmic loops and tail of the AT 2 receptor are used to determine what regions are important to G-protein activation. These studies will map the G-protein coupling regions of the human AT2 receptor and develop a wider …
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