Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"Amber suppression"”.
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Identification and Evolution of New Orthogonal Aminoacyl-tRNA Synthetase/tRNA Pairs for Genetic Code Expansion
… pairs, 3 of which displayed high activity in amber suppression, the technique of choice used to implement genetic code expansion in model organisms. I additionally evolved new amino acid substrate specificities for two pairs. Finally, I use tREX to characterize a matrix of 64 orthogonal …
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Metalloprotein engineering with unnatural amino acids: application in functional heme-copper oxidase and azurin
… ligation, expressed protein ligation and amber suppression. They all have their own advantages and disadvantages. Heme copper oxidases (HCOs) catalyzes reduction of O2 to H2O and harvest energy at the end of respiration chain. The enzyme plays a pivotal role in aerobic respiration and thus …
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Control of Salmonella Virulence by Microbiota-Derived and Dietary Fatty Acids
… is short-chain fatty acylated. I employ Amber Suppression Technology and CRISPR-Cas9 genome editing to faithfully mimic butyrylation on endogenous HilA. Biochemical and functional characterization show that acylation of HilA has site-specific effect, and K90 butyrylation affect HilA …
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Biochemical and Structural Insights into VCP Unfoldase and WRN Helicase Complexes
… my efforts to establish a method combining amber suppression, photo-crosslinking, and quantitative proteomics that identifies domain-specific VCP interactors. Interestingly, we identify profilin-1 (PFN1), an actin monomer-binding protein, as a top hit in one of our proteomics datasets. We …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
Flaviviruses are arthropod-borne viruses that constitute a significant global public health burden. The envelope protein (E) orchestrates the processes of virus assembly, budding, and entry into cells and is the primary target of neutralising antibodies. The E proteins arranged on the surface of …