Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Alternating access mechanism"”.
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Molecular insights into alternating access mechanism of secondary active transporters from molecular dynamics simulations
… its function, transporter operates via an alternating-access mechanism, in which it undergoes structural transitions between outward-facing (OF) and inward-facing (IF) states, to translocate substrate from one to the other side of the membrane. The alternating access mechanism has received …
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Mechanistic studies of ABC transporters
… facilitate the substrate translocation with an alternating-access mechanism. The architecture of ABC transporters contains two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs) locating at the cytoplasmic side of the membrane. While the TMDs provide the pathway for substrate …
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Functional and mechanistic studies of human mitochondrial carriers
… binding and proton coupling, and the kinetic mechanism. The aim of this thesis was to address some of these controversies by understanding the properties of specific proteins, and by extension the mitochondrial carrier family as a whole. To that end, the verification of SLC25A51 as a …
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Mechanistic insights into the secondary-active multidrug transporter LmrP
… crisis and one of the important contributing mechanisms is active drug extrusion by bacterial multidrug transporters. LmrP is a Major Facilitator Superfamily (MFS) multidrug transporter which, when overexpressed in bacteria, can confer resistance to 22 clinically important antibiotics. The …
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Investigating the substrate binding mechanism of the mitochondrial ADP/ATP carrier
… and matrix-open states support an alternating access mechanism, involving a cytoplasmic and a matrix gate. However, the molecular nature of substrate binding remains unresolved. Substrate binding is concomitant with the structural movements required for translocation, because …
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Atomistic insights into the structure, dynamics, and regulation of integral membrane proteins
… an indispensable tool for uncovering atomistic mechanisms that govern biomolecular function. The unique ability of MD to resolve molecular motions at high spatial and temporal resolution offers mechanistic insights into dynamic interactions that are often inaccessible to static structural …
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Mechanism and energetics of membrane transporters and channels
… membrane transporters use the widely-accepted ""alternating-access mecha- nism"", which ensures that the substrate is only accessible from one side of the membrane at a given time, and relies on complex protein conformational changes between outward- facing (OF) and inward-facing (IF) states, …