Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 3 of 3 for “"Alpha-mating factor"”.
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The structural characterization of the Saccharomyces cerevisiae alpha mating factor secretion signal for recombinant protein secretion in Pichia pastoris
<p>The methylotrophic yeast Pichia pastoris has been used extensively for expressing recombinant proteins because it combines the ease of genetic manipulation with rapid growth to high cell densities and provides complex posttranslational modifications. The most successful and commonly used …
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Structural characterization of the MATα prepro-peptide secretion leader in Pichia pastoris
<p>The methylotrophic yeast, Pichia pastoris, is the most successful and favored microbial eukaryotic expression system for the production of recombinant proteins for biopharmaceutical or industrial purposes. P. pastoris has the ability to produce foreign proteins at high levels extracellularly, …
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The effects of cis- and trans-acting mutations on recombinant protein secretion in Pichia pastoris
… Multiple tools such as the cis -acting factors MATα secretion signal and MBP fusion partners, and trans-acting modifications such as the bgs mutants have increased heterologous protein secretion. Although these techniques have already been used, their effects on the protein secretory …