Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"Alpha-helix"”.
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Energetics of [alpha]-helix formation in peptides and proteins
This thesis focuses on the energetics of !-helix formation in peptides and proteins. The [alpha]-helix is the most prevalent type of secondary structure found in proteins, and has arguably dominated our thinking about protein structure since its discovery, as it plays an important role in the early …
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Alpha helix 7 of PabB functions in catalytic activity and subunit interaction in Escherichia coli.
Alpha helix 7 of PabB functions in catalytic activity and subunit interaction in Escherichia coli.
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Covalent Modification that Enhances Protein Properties and Functions and Folds Random Coil into Alpha-Helix
… alone folded the random coil into an <em>a</em>-helix. The COMPcc is capable of binding small molecule such ascurcumin, retinol and vitamin D. Curcumin exhibited an increase in the fluorescence signal upon binding to L44A(betaCD)<sub>n</sub> as compared to the wild type protein suggesting that …
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Characterization of an Amphipathic Alpha-Helix in the Membrane Targeting and Viral Genome Replication of Brome Mosaic Virus
… examined the possible role of an amphipathic α-helix, helix B, in BMV 1a's membrane association. Deletion or single substitution of multiple amino acids of helix B abolished BMV 1a's localization to nuclear ER membranes. Additional reporter-based, gain-of-function assays showed that helix B is …
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Conformational and Orientational Dynamics of Semi-Rigid Macromolecules in Shear Flow
… in shear flow. Poly-L-lysine exists as an $\alpha$-helix at high pH and a random coil at neutral pH. When the $\alpha$-helix is heated above 27$\sp\circ$C, the macromolecule undergoes a conformational transition to a $\beta$-sheet. In this study, the stability of the secondary structure of …
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Direct observation of fast protein folding: Distinct nanosecond and microsecond events in the folding of apomyoglobin
… tryptophan fluorescence (residue 14 in the A alpha helix) serves as a local probe of the A-helix and the disposition of the H alpha helix. Methionine (residue 131 in the H-helix) quenching of tryptophan fluorescence at the interface of the A- and H-helices in the compact or native-like …
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Investigation of the Role of Amide I to Amide II Ratio and Alpha Helix to Beta Sheet Ratio of Faba Bean Seeds in the Determination of Microbial Protein Synthesis and Animal Performance and Metabolism in Ruminant Livestock Systems.
… influence of amide I to amide II ratio, and α-helix to β-sheet ratio (α_β) on the protein quality (solubility and digestibility), metabolism, and bioavailability in dairy cows has not been established. Thus, the objective of this research was to evaluate the role of the protein-related spectral …
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Protein -Protein Interactions of Synucleins: Implications for Normal Function and Neurodegenerative Disease
… peripheral nervous systems. The three isoforms, alpha, beta, and gamma, are most homologous in their N-terminal ∼100 residues, which form a lipid-binding amphipathic alpha-helix. Alpha-synuclein (AS) has been implicated in learning and memory and neurodegenerative disease, thereby influencing …
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Sequence-Based Properties that Identify Intrinsically Disordered Phase-Separating Protein Regions
… propensities, like preferences for alpha-helix, beta-turn, or beta-sheet. These results established that a hydrophobicity scale could accurately distinguish between folded and ID populations, and that an alpha-helix scale paired with v could optimally identify PS-IDR from IDR. Using …
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Early Events in the Folding of the Protein Ubiquitin
… time, and a transient structure containing more alpha-helix than the native state has been observed during the protein's folding.
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Machine learning applications for the topology prediction of transmembrane beta-barrel proteins
… provide. The two most predominant classes are alpha-helix bundles and beta-barrel transmembrane proteins. Alpha-helix proteins are present in larger numbers than beta-barrel transmembrane proteins in structure databases. Therefore, there is a need to find computational tools that can predict …
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A Peptide-Based Platform for Displaying Antibodies to Engage T Cells
… analysis revealed that EAKH6 adopts a mixed alpha-helix/bata-strand conformation. In the presence of EAK, EAKH6 exists predominantly in a beta;-strand conformation. Using nickel-bound horseradish peroxidase as a probe, the composite of EAK-EAKH6 was found to display His-tags. T-cell-specific …
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Struktur, Funktion und Allergenität Bet v 1-homologer Proteine: Das Allergen Gly m 4 und das Enzym Norcoclaurin-Synthase
… und zwei kurze V-förmig angeordnete alpha-Helices, die zusammen mit einer langen C-terminalen alpha-Helix einen hydrophoben Hohlraum umschließen. Die in dieser Arbeit mittels magnetischer Kernspinresonanz-(NMR-)Spektroskopie im Detail bestimmte Struktur des Bet v 1-Homologs Gly m 4 …
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Model membrane interactions with ions and peptides at the air/water interface
… It is unstructured in solutions and forms alpha-helix at the air/water interface or in the membrane bound state. The peptide has affinity to the negatively charged lipids and even can fold into beta-sheet structure in the vicinity of charged membranes at high peptide to lipid ratio. It was …
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Insights into p53-Dependent Apoptotic Signaling and Cell Fate vis-a-vis Functional Cooperation among BCL-xL, Cytoplasmic p53, and PUMA
… and the PUMA BH3 domain residues fold into an alpha helix and subtly remodel BCL‑xL to trigger its dimerization. Wild type PUMA or a PUMA mutant incapable of promoting BCL‑xL dimerization (PUMA W71A) equivalently inhibit the anti‑apoptotic BCL‑2 repertoire to sensitize for death …
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Analyse der Carboxypeptidase D-unabhängigen und -abhängigen Schritte bei der Infektion mit dem Hepatitis-B-Virus der Ente
… von preS (AS 86 - 115), der eine amphipathische alpha-Helix umfasst, und dann linear fortschreitend an N-terminal davon gelegene Aminosäuren (AS 30 - 85), woraus sekundär ein hochaffiner Komplex resultiert. Um die funktionelle Bedeutung der Interaktion von preS mit dCPD zu untersuchen, wurden …
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Biological effects of synthetic biomolecules that may either mimic or antagonise the function of hCG in the development of cancer and autoimmune disease
… aptamers that specifically bind to the Beta and Alpha-helix regions of the hCG receptor, modulating its signalling. Peptide aptamers are a novel class of molecules with high binding specificity and can function as either agonists or antagonists. Naïve CD4+ T cells and B cells were isolated from …
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Supercharging: An Investigation into the Effects of External Amino Acid Residue Charge on the Solubility and Internal Electric Character of Bound Ligands in a Heme-Binding de novo-Designed Protein
… negative charge resulted in loss of the alpha-helix character essential to the protein’s heme-binding function. More moderately charged proteins demonstrated that solubility was significantly enhanced in net negatively charged proteins. We were also able to demonstrate a direct …
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Overexpression, purification and characterization of human proapolipoprotein A-I and mutants
… or proapoA-I were used. Furthermore, their $\alpha$-helical contents were the same, they had similar fluorescence properties and activated LCAT equally well. In conclusion, proapoA-I expressed and purified from E. coli is functionally and structurally indistinguishable from apoA-I purified …
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Molecular Dissection of the Ubiquitin-Independent Degradation Signal At the Unstructured Amino Terminal End of Human Thymidylate Synthase
… and that this region, in cooperation with an alpha-helix formed by the next 15 residues, functions as a degron, i.e., it is capable of destabilizing a structurally unrelated protein to which it is N-terminally fused. However, the specific amino acids or chemical characteristics of the …
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