Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"Alpha-chymotrypsin"”.
-
Kinetics and mechanism of alpha-chymotrypsin catalyzed reactions.
The principle of microscopic reversibility was formulated for elementary reactions at equilibrium. It follows from the principle that for any elementary reaction the favored reaction path in one direction must be the reverse of that in the opposite reaction, and that the ratio of rate constants is …
-
Theoretical and experimental studies of slowly deacylating alpha-chymotrypsin acyl enzymes
The differential deacylation rates for alpha-chymotrypsin beta-phenyl acyl enzymes 13 and 14 have been studied using the techniques of molecular mechanics and molecular dynamics. These studies indicate that the ketone side-chain of slowly deacylating acyl enzyme 13 is hydrogen bonded to the NH of …
-
Halo enol lactone and protio enol lactone inhibitors of alpha-chymotrypsin: Mechanism and stereospecific behavior of inhibition
A kinetic study of inactivation of $\alpha$-chymotrypsin by enol lactones was performed. Halo enol lactones, 3-(1-naphthyl)-6(E)-(iodomethylene)tetrahydro-2-pyranone ($\alpha$Np6I) and 3-phenyl-6(E)-(bromomethylene)tetrahydro-2-pyranone ($\alpha$Ph6Br), showed burst kinetics in the inactivation of …
-
PART I. STEREOELECTRONIC EFFECTS IN PHOSPHATE ESTERS. PART II. PROTON NUCLEAR MAGNETIC RESONANCE STUDY ON THE BINDING OF TRANS-CINNAMALDEHYDE TO ALPHA-CHYMOTRYPSIN.
… STUDY ON THE BINDING OF TRANS-CINNAMALDEHYDE TO ALPHA-CHYMOTRYPSIN.
-
Halo Enol Lactones as Enzyme-Activated Irreversible Inhibitors of Alpha-Chymotrypsin: I. The Effect of Lactone Substitution Pattern. Ii. Alpha - Amino-Functionalized Lactones
… irreversible (suicide) inhibitors for (alpha)-chymotrypsin with the activities of the corresponding (alpha)-substituted lactones. The 4-phenyl-5(E)-(iodomethylidene) tetrahydro-2-furanone, the 4-phenyl-5(E)-(iodomethylidene)dihydro-2-furanone, the …
-
2-Pyranones as Suicide Inhibitors for Alpha-Chymotrypsin: I. A Structure-Activity Study of the 6-Halo-2-Pyranones. Ii. 5-Halomethyl-2-Pyranones as Inhibitors of Chymotrypsin
… of 6-halo-2-pyranones as suicide inhibitors of chymotrypsin. In the 6-chloro-2-pyranone series, substitution in the 3- position of the pyrone by either ethyl or n-butyl produced poor binding relative to 3-benzyl-6-chloro-2-pyranone, and no inactivation. Increasing the aromatic area of the …
-
A novel polyelectrolyte complex between amphiphilic poly (allyl amine) and sodium alginate.
… oral delivery - namely its enzymatic cleavage by alpha-chymotrypsin. Poly (allyl amine) was prepared from poly (allyl amine) hydrochloride. Amphiphilic derivatives were prepared using palmitic acid-N-hydroxy succinamide, and resulted in 3.7% and 5.1% palmitoyl-grafted poly (allyl amine), based on …
-
1-Acylamido boronic acids and Difluoroborane analogs of amino acids: Synthesis and biological activity of transition state analogs for Chymotrypsin and Elastase (inhibition, esters)
… state"" inhibitors of the serine proteases (alpha)-chymotrypsin and elastase. These inhibitors were designed to resemble N-acyl phenylalanine, phenylglycine (as chymotrypsin inhibitors), alanine, valine, and isoleucine (as elastase inhibitors) and have boron sybstituted for the carboxy …
-
The synthesis and evaluation of halo and protio enol lactone Pro-Val pseudodipeptides as potential inhibitors of human leukocyte elastase
… inhibitors of the serine protease $\alpha$-chymotrypsin have previously been prepared from aryl-substituted halo and protio enol lactones. In this study, the enantiomerically pure disubstituted protio and halo enol lactones 11a-c and 12a-b were prepared as potential inhibitors of the …
-
Hydrogels for Biomacromolecule Immobilization and Sensing
… containing peptides that were responsive to alpha-chymotrypsin (CKYC) and botulism neurotoxin (CSNKTRIDEANQRATK{Nle}LC) were successfully synthesized and copolymerized into polyacrylamide hydrogel networks. When exposed to buffered solutions that contain their sequence specific protease, the …
-
Halo Enol Lactones as Enzyme-Activated Irreversible Inhibitors of Chymotrypsin: Their Synthesis, Kinetics, and Mechanism of Inactivation (Suicide Substrates)
… inhibitors or suicide substrates for (alpha)-chymotrypsin. Acyl transfer to the active site serine generates a halo methyl ketone that remains tethered in the catalytic site during the lifetime of the acyl enzyme, available for reaction with an enzymatic nucleophile. To investigate the …
-
Modification of soybean proteins by immobilized proteases
Trypsin and alpha-chymotrypsin were immobilized on nylon pellets or porous glass by covalent methods to change molecular properties and functional characteristics of soybean proteins. The amount of trypsin immobilized on nylon pellets using the glutaraldehyde method was high when the pellets were …
-
Novel polyelectrolyte complexes for oral insulin delivery.
… protect insulin from degradation by trypsin and alpha-chymotrypsin, but not from pepsin. In-vitro mucin adsorption assays showed that all polymers exhibited a similar mucoadhesive profile with their corresponding insulin PEC, with thiolated Paa derivatives adsorbing greater than 20% more mucin …
-
Non-invasive drug delivery to the posterior segment of the eye: Development of formulation strategies and evaluation of ocular distribution and pharmacological activity
… were evaluated for IOP reduction in an alpha chymotrypsin induced rabbit glaucoma model. With the 0.5%w/v THC emulsion formulation drop in IOP was observed at 30 min, but was very minimal and short acting (60 min). With an increase in dose (0.8% w/v THC) a similar effect was observed …