Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Alpha-actinin"”.
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Evolutionary Dynamics of the Alpha-actinin Gene Family
The alpha-actinin gene family is a member of the spectrin superfamily of proteins. Other members of this superfamily include the a- and ~-spectrins, ~heavy spectrin and dystrophin. Each member of this superfamily has a unique actin cross-linking distance consequent to the specific number of …
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alpha-Actinin-4 and the podocyte: Implications for focal segmental glomerulosclerosis
… of FSGS by expressing a high-affinity variant of alpha-actinin-4 (K256E) in a podocyte-specific manner. Transgenic mice display podocyte damage, subsequent loss of serum proteins into the urine (proteinuria), and glomerular sclerosis, similar to that observed in human patients. In cultured …
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A study on the modulation of alpha-actinin and filamentous actin
… extracellular matrix. Here I demonstrate that α-actinin-4, a well-known actin crosslinking protein and long believed to be the primary actin bundling protein for contractile actin arrays, actually inhibits bundling of actin filaments into stress fibers in epithelial cells. I show that α-actinin-4 …
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Dynamic viscoelasticity of actin networks cross-linked with wild-type and mutant [alpha]-actinin-4
The actin cross-linker [alpha]-actinin-4 has been found indispensable for the structural and functional integrity of podocytes; deficiency or alteration of this protein due to mutations disturbs the cytoskeleton and results in kidney disease. This thesis presents rheological studies of in vitro …
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Dynamics of Alpha5 Integrin, Paxillin, and Alpha-Actinin During Formation and Disassembly of Adhesions in Migratory Cells
… protein expressed by cells transfected with alpha5-GFP is correctly localized, is surface-expressed in a heterodimeric form, and restores the ability of a cell line void of alpha5 expression to adhere and to spread on a fibronectin substrate. By viewing and comparing images taken from …
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Strukturen der Kraftübertragung im quergestreiften Muskel : Protein-Protein-Wechselwirkungen und Regulationsmechanismen
… an die beiden Liganden N-RAP und alpha-Actinin. Die molekulare Grundlage der HCM-verursachenden Mutationen im MLP-Gen könnte folglich eine Veränderung der Homöostase im ternären Komplex MLP – N-RAP – alpha-Actinin sein. Die Expressionsdaten eines neu generierten …
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Effects of cross-link and myosin motor concentrations on active muscle gel contraction time and extent
… with the concentration of myosin motors and alpha-actinin crosslinks.
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The effects of force on actin-myosin ATPase activity
… was imposed on a sliding actin filaments using alpha-actinin, and the corresponding effects of load were determined using a novel experimental and theoretical protocol. Our results show that mechanical loads decrease both actin-myosin attachment and detachment kinetics during actin sliding in …
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Tyrosine phosphorylation and cytoskeletal tension regulate the release of fibroblast adhesions
… remaining cells appear contracted with talin, $\alpha$-actinin and vinculin localized diffusely throughout the cell. Integrin containing tracks of variable intensity outline the regions where cells had resided before they detached from the substratum. At lower ATP concentrations (0.5-5mM) the …
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Junction Specificity and Transmembrane Linkages of Integrins
… the sarcomeric architecture was positive for $\alpha$-actinin. Together, the data indicate the P4B2 antigen is part of a unique GAPDH containing protein complex that may be involved in reinforcement of established cytoskeletal structures.
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UNDERSTANDING THE MOLECULAR BASIS OF CELL DEATH MEDIATED BY ONCOSIS-INDUCING CYTOTOXIC ANTIBODIES
… OF ACTIN-ASSOCIATED PAXILLIN, TALIN AND ALPHA-ACTININ. THE RESULTANT MEMBRANE INJURY WAS LIKELY EXACERBATED BY THE LOSS OF MYOSIN II ACTIVITY NECESSARY FOR MEMBRANE REPAIR. CYTOTOXICITY ALSO REQUIRED CROSSLINKING OF SURFACE GLYCOPROTEINS BY THE MABS AS MONOVALENT FAB FRAGMENTS DID NOT …
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ACTN3 R577X GENOTYPES ASSOCIATE WITH CLASS II AND DEEP BITE MALOCCLUSIONS
Alpha-actinins are myofibril anchor proteins, which influence contractile properties of skeletal muscle. ACTN2 is expressed in slow type I and fast type II fibers whereas ACTN3 is expressed only in fast fibers. ACTN3 homozygosity for the 577X stop codon (i.e. changing 577RR to 577XX - the R577X …
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Protein Biomarkers of Ionizing Radiation and Standardization by MALDI MS/MS and Quantification
… mitochondrial precursor (down-regulated), alpha-actinin-2 (down-regulated), pyruvate kinase isozymes M1/M2 (down-regulated) and ATP synthase subunit beta, mitochondrial precursor (down-regulated).
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Rigidity and Fluidity in Living and Nonliving Matter
… with their myosin-driven contractility and alpha-actinin-driven extendability, while the friction coefficients of the two beads describe the catch/slip bond behavior of the integrins in focal adhesions. Net motion arises from an interplay between active contractility (and passive …
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The effect of calcium activated protease (CAF) and cathepsin D on bovine muscle myofibrils under varying conditions of pH and temperature
… release of soluble protein, including intact alpha-actinin, from the myofibrils was observed. SDS-PAGE analysis of treated sedimented myofibrils showed CAF digestion had degraded titin and, at least partly, tropomyosin, and troponins T and I. A trio of bands in the 30,000-dalton region …
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Visualizing Cell Migration in Situ
… encoding GFP-fusion proteins demonstrate that alpha-actinin resides in linear, punctate structures; the alpha5 integrin, while not generally well organized, resides in some small, focal complex-like concentrations and in vesicles, which can localize at the base of protrusions; and paxillin is …
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Organization of Postsynaptic Proteins via the Neuronal Cytoskeleton
… In contrast, the synaptic clustering of CaMKIIalpha, another core component of the PSD, was completely dependent upon an intact actin cytoskeleton and was partially disrupted by detergent. Drebrin and alpha-actinin-2, actin-binding proteins concentrated in spines, were dependent upon F-actin …
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Integrin Alpha Subunit Ratios, Cytoplasmic Domains, and Growth Factor Synergy Regulate Muscle Proliferation, Differentiation, and Signaling
… was addressed by ectopic expression of integrin alpha subunits in primary quail skeletal muscle. Ectopic expression of either the human or $\alpha$5 or the chicken $\alpha$6 subunit produced contrasting phenotypes. The $\alpha$5 transfected myoblasts remain in the proliferative phase and are …
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The zebrafish maternal factor pollywog is required for yolk syncytial layer morphogenesis
… Incuded in this thesis is work highlighting the alpha-actinin gene family in zebrafish. alpha-actinins are actin microfilament crosslinking proteins. Vertebrate actinins fall into two classes: the broadly-expressed actinins 1 and 4 ( actn1 and actn4 ) and muscle-specific actinins, actn2 and actn3 …
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