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Showing 1 to 20 of 39 for “"Alizarin"”.
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The synthesis and investigation of alizan-based functional chromogenic probes for heavy mental sensing in aqueous medium
… of laborious sampling. Sensor AB and EF, an alizarin derivative was synthesised and characterised using UV-vis, 1H-NMR and FT-IR spectroscopy. The aim of this study was to investigate a colorimetric method based on Schiff base synthesised sensors and other probes such as ready-to-use alizarin …
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Analytical Separation of Zirconium-95 and Niobium-95 from Uranium Irradiated by Thermal Neutrons
… Fluorescence and a colorimetric method using Alizarin Red-S. Alizarin Red-S was not found suitable for this research due to the interference of niobium, so the other techniques mentioned above were used. The results are summarised in tables which show the percentage of each element in aqueous …
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Surface enhanced resonance Raman spectroscopy of dyes: semi-quantitative trace analysis
… between two structurally similar anthraquinones, alizarin and purpurin, using SERRS. It was also possible to demonstrate the existence of multiple molecular species of certain dye molecules, as a function of pH e.g. nuclear fast red, metanil yellow, purpurin and alizarin. For some dye molecules …
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Effect of Implant Surface Microtopography by Hydroxyapatite Grit-Blasting on Adhesion, Proliferation and Differentiation of Osteoblast-like Cell Line, MG-63
… ALP activity assay, mineralization assay using alizarin red-s staining and gene expression of osteoblastic differentiation marker using RT-PCR or ELISA. Results: Comparing with machined group, osteoblastic cell adhesion, proliferation and ALP activity of RBM surfaces were shown higher. Also, …
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Comparative osteogenesis of equine mesenchymal stem cells isolated from bone marrow, adipose tissue, and synovium
… evaluated by staining for mineralized matrix (Alizarin Red and Von Kossa) and the enzymatic activity of alkaline phosphatase (ALP). Other secondary indicators of osteogenic differentiation included in this study were mRNA levels of ALP and osteoblast-specific genes Runx2, Osterix, Osteonectin …
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Effects of Neurotrophic Factors on Osteoblast Growth and Differentiation
… levels, and mineralization was measured by alizarin red staining. Although we did not observe effects of CGRP or SP on cell growth, we did observe effects on differentiation—a 48 hour exposure to CGRP induced a dose-dependent increase in alkaline phosphatase levels in the calvarial …
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Rooibos tea flavonoids increase mineral content in human osteoblast-like cells
… Saos2 cells. Mineral was quantified by alizarin red staining and characterized by quantifying alkaline phosphatase (ALP) activity, cell mitochondria activity and toxicity, in addition to changes in regulatory markers of osteoblastic activity. Rutin (≥50μM), hyperoside (≥5.0μM), orientin …
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The synthesis and properties of some chelating ion exchangers.
… chelating polymers containing salicylic acid, alizarin or pyridyl azo resorcinol as the functional group have also been examined to determine their behaviour with the same six metals. An attempt to determine the magnitude of the stability constants for the metal-resin complexes, uranyl and …
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Isolation and characterisation of canine osteosarcoma cells to investigate mechanisms of chemoresistance
… Kinase Inhibitor to encourage cell attachment. Alizarin Red Staining, alkaline phosphatase (ALP) assay and qPCR on differentiation markers were performed to determine the osteoblastic characteristics of the cells. To determine the chemosensitivity of the three cell types to doxorubicin and …
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Novel Electrochemical Methods for Acidity Monitoring: Theory, Design and Application
… of test). It was then cross connected with an alizarin electrode, forming the pH sensor free from a glass reference electrode. Chapter 6 describes novel designs of hydrodynamic pH sensors. The design of a microfluidic pH sensor modified by poly-sodium salicylate was firstly demonstrated. The …
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Sequência de ossificação craniana de Aparasphenodon brunoi Miranda-Ribeiro, 1920, com comentários sobre o condrocrânio e o desenvolvimento da ornamentação óssea (Amphibia, Anura, Hylidae)
… and juveniles, cleared and alcian blue - alizarin stained. The animals were staged using Gosner (1960) for tadpoles, and the extension and degree of cranial ornamentation for froglets and juveniles. The tadpole, the chondrocranium at different developmental stages, the sequence of cranial …
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Predispersed solvent extraction
… (CGA). Copper, uranium and chromium ions, and alizarin yellow were extracted from very dilute aqueous solutions using PDSE. Tests were performed in a vertical glass column in both batch and continuous modes, and in a continuous horizontal trough. The new solvent extraction procedure worked very …
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Investigation of adipose-derived mesenchymal stem cells interaction with electrospun demineralized bone matrix nanofiber scaffolds
… one week. Scanning electron microscopy (SEM) and alizarin calcium staining techniques were used to examine mineralization patterns qualitatively on DBM and PCL nanofiber scaffolds. DBM scaffolds deposited more calcium mineral than PCL scaffolds during three-week experiments. Mineralization was …
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Biochemische und funktionelle Charakterisierung einer gerichteten Gendeletion (Knockout) von Fetuin-A in Mäusen
… DBA/2 (D2) in contrast to D2 wildtype mice. Alizarin red staining of adult mice detected calcium precipitates in heart, lung, kidney, sexual organs, skin and tongue. The damage of the affected organs led also to an increased mortality and a depressed breeding performance. The renal damage was …
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The Central Stoneroller, Campostoma Anomalum, as an Indicator of Heavy Metal Contamination Using Otolith Age Determination and Growth Analysis
… reference site. A lab experiment revealed that alizarin red S will impart a violet-red mark in stoneroller otoliths that can be viewed with a compound light microscope. Using that dye, a second experiment validated the formation of daily increments in stonerollers provided different food …
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MyosinX is Required For Craniofacial Development in Danio Rerio
… in <em>myosinX</em> deficient embryos. Using alizarin red staining techniques, pharyngeal tooth development was also examined. Tooth development occurs on the fifth ceratobranchial arch in a three crown clustered manner. However, in <em>myosinX</em> deficient zebrafish, pharyngeal crown …
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Effects of acetylsalicylic acid on odontogenesis of human dental pulp cells and TGF-ß1 liberation from dentin
… RUNX2 mRNA levels were measured with RT‐qPCR. Alizarin red staining was conducted to evaluate the mineralized nodule formation. Dentin slices were submerged in PBS (negative control), 17% EDTA (positive control), and ASA before collecting the solution for TGF-ß1quantification by ELISA. The data …
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Saccharide recognition : boronic acids as receptors in polymeric networks
… to phenylboronic acid. In addition, the use of Alizarin Red S as an electrochemical reporter was described for the first time to monitor the saccharide binding to arylboronic acids not only with spectroscopy. Here, the redox behaviour and the displacement were recorded by cyclic voltammograms. …
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Histopathologische Untersuchungen von Aneurysmen der Aorta ascendens in Abhängigkeit von Aortenklappenvitien
… atherosclerosis, von-Kossa (vKossa) stain and alizarin-red (Ared) stain were used as calcinosis specific dyes. HE stained samples were analyzed using a semi-quantitative score, while calcinosis quantification was carried out by a binary, pixel guided contrast analysis via computer software. …
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In Vitro Assessment of Bone and Cartilage Stimulating Peptides
… differentiation marker) expression assay, an Alizarin Red S staining assay and a Boyden Chamber assay, respectively. Osteoprogenitor cells examined included MC3T3-E1 preosteoblasts, human bone marrow-derived mesenchymal stem cells (hBM-MSCs), human periosteum-derived stem cells (hPDSCs), and …
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