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Showing 1 to 12 of 12 for “"Alicyclobacillus"”.
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Understanding Alicyclobacillus Spoilage at the Intersection of Genotype and Environment
… Beverages are known to be spoiled by Alicyclobacillus spp., which are extremophilic sporeformers that cannot be killed by traditional pasteurization methods. The spoilage metabolite guaiacol is only produced by a subset of Alicyclobacillus spp. under specific beverage storage …
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The prevention of spoilage in fruit juices by alicyclobacillus acidoterrestris and propionibacterium cyclohexanicum
During the past two decades several novel spoilage micro-organisms have emerged. Raw materials and products have been contaminated in increasing numbers of spoilage incidents causing widespread problems within the juice and beverage industry. This study investigates two such spoilage …
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Combinatorial effects of bovicin HC5 and epsilon-polylysine against Alicyclobacillus acidoterrestris in orange juice
Alicyclobacillus acidoterrestris is a thermo-acidophilic spore-forming bacterium that can survive pasteurization procedures and cause spoilage of fruit juices and acidic beverages in the food industry. Antimicrobial peptides (AMPs) are considered an alternative strategy to control the growth of …
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The Efficacy of Antimicrobials for the Control of Alicyclobacillus acidoterrestris in Fruit and Vegetable Juices
The efficacy of antimicrobials for control of A. acidoterrestris spoilage in juices was analyzed. Apple and tomato juices were inoculated with 4 log spores/ml. Antimicrobials were added at: 1000, 500 and 250 ppm (sodium benzoate, potassium sorbate, and sodium metabisulfite); 500, 250, and 125 ppm …
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Strukturelle und mechanistische Arbeiten an Triterpen-Cyclasen aus Alicyclobacillus acidocaldarius und Arabidopsis thaliana sowie Kristallstrukturen der katalytischen Fragmente von AB-Toxinen aus Clostridium difficile und Bacillus sphaericus
… Kristallstruktur der Squalen-Hopen-Cyclase aus Alicyclobacillus acidocaldarius (baSHC) im Komplex mit dem Substratanalogon 2,3-Dihydro-2-azasqualen gelöst werden. Die bei einer Auflösungsgrenze von 2.1 Å ermittelte Kristallstruktur zeigte den Bindungsmodus des Inhibitors im Detail und erlaubte …
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Esterase 2-oligodeoxynucleotide conjugates as enzyme reporter for electrochemical detection of DNA and identification of bacterial species
… reporter is expected. Esterase 2 (EST2) from Alicyclobacillus acidocaldarius was introduced and mutated to have an accessible cysteine residue at 118th codon. This esterase was purified by a single-step affinity chromatography with trifluoromethyl ketone as a ligand and covalently conjugated …
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Versatile, automated sample preparation and detection of contaminants and biological materials
… DNA extraction of food bacteria (E.coli and Alicyclobacillus) was optimized inside the LabTube for 102-109 inserted DNA copies. The extracted DNA was amplified using the qualitative isothermal LAMP method and semi-quantitative, real-time PCR inside the LabReader. The combined extraction and …
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Elektrochemische Detektion von RNA mittels Nukleinsäurehybridisierung
… des thermostabilen Reporterenzyms Esterase 2 aus Alicyclobacillus acidocaldarius durch eine Ein-Schritt Sandwich-Hybridisierung nachgewiesen. Ein Spezies-spezifisches Oligodesoxynukleotid wird auf der Goldelektrode eines elektrochemisch-auslesbaren Biochips immobilisiert und bindet die bakterielle …
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Characterization of SsoSSB, Sso1450, Sso2001 Proteins and Analysis of CRISPR and cas Genes from Sulfolobus solfataricus P2
… gene was fused with an esterase gene from Alicyclobacillus acidocaldarius, and was co-expressed in a soluble form. The enzymatic screening indicated that Sso2001 harbored a nuclease activity. Further experiments showed that Sso2001 was an endonuclease with specificity for cleavage near G …
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Rapid separation of phenolic flavourings by liquid chromatography and nanomolar electrochemical detection at a boron-doped diamond electrode
In recent years, there has been considerable research focus on natural plant phenolics. They are present as key contributors to the flavour of food and beverages and are also proven to function as antioxidants, providing a wide range of physiological properties including anti-cancer, …
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Towards bioengineered ecosystems: Three studies in invasion biology
… Second, experiments with the acidophile Alicyclobacillus acidoterrestris showed that while acid-tolerant invaders exist, the presence of environmental hurdles such as low temperature and high osmolarity are major factors in preventing their establishment in sourdough cultures. Finally, a …