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Showing 1 to 17 of 17 for “"Alginolyticus"”.
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Regulation of nitrogen metabolism in vibrio alginolyticus
Aerated cultures of Vibrio alginolyticus produced histidase at 30°C but production of histidase was repressed by either incubation at 37°C or a lack of oxygen. A similar regulation system by temperature and oxygen has been reported for collagenase and prntease production by V. alginolyticus (Hare …
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Genetic and biochemical studies of Vibrio alginolyticus glutamine synthetase
A genomic library of the collagenolytic Vibrio alginolyticus strain was established in Escherichia coli HB101 employing the positive selection vector pEcoR251. A glutamine synthetase (GS) gene, glnA was identified by complementation of the glnA deletion in E. coli ET8051 glnA, glnL, glnG deletion …
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Studies on the regulation of extracellular collagenase production by vibrio alginolyticus
Vibrio alginolyticus synthesized extracellular collagenase in a highly aerated peptone medium at the late-exponential and early-stationary phases of growth. Collagenase synthesis was subject to end-product repression and was repressed by various amino acids and ammonium ions. Glutamine caused …
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Molecular biology studies on the extracellular serine proteases of Vibrio alginolyticus
Vibrio alginolyticus is a gram-negative aerobic bacterium that produces several extracellular serine proteases and a collagenase during the stationary growth phase. The aim of this study was to investigate alkaline serine protease production by this organism, and to attempt the cloning and …
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Characterization and regulation of serine exoproteases and collagenase in vibrio alginolyticus
… collagenase and serine proteases by Vibrio alginolyticus during stationary phase was inhibited by a temperature shift from 30 to 37°C and by lack of oxygen. V. alginolyticus had identical growth rates at 30 and 37°C. Aeration did not affect the growth rate of stationary phase cells when the …
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Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli
… halotolerant, gram-negative organism Vibrio alginolyticus. The cloning, expression and regulation of the v. alginolyticus sucrose utilization system in Escherichia coli is discussed and the results of a molecular analysis of the sucrase gene (scrA) are presented. The clone pVSlOO, containing …
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Molecular analysis of the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli
… halotolerant, Gram-negative bacterium Vibrio alginolyticus. The V. alginolyticus sucrose utilization system originally cloned into Escherichia coli on plasmid pVS100 involves a sucrase enzyme (gene scrB), and a sucrose uptake system. Synthesis of the sucrase and sucrose uptake system in V. …
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A Genetic and Physiological Study of the Role of Extracellular Copper-Binding Proteins in Copper Detoxification by the Marine Bacterium <i>Vibrio alginolyticus</i>
<p>Supernatant proteins in <em>Vibrio alginolyticus</em> batch cultures were analyzed by SDS-PAGE before copper was added, 24 and 48 hours after the addition of copper, and in 24 hour control (no Cu) cultures. Two proteins, one 21 kilodalton (kDa) and one 19 kDa, were found to be copper-induced, …
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Dynamics of semi-flexible fibres in viscous flow
… semi-flexible fibres. Bacteria such as Vibrio alginolyticus and Escherichia coli swim by rotating one or more helical flagella. Vibrio alginolyticus has only one flagella and changes direction by altering its sense of rotation. Experimental observations of Vibrio alginolyticus have found that …
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Elucidating Collagen Degradation Synergy between Col G and Col H from Hathewaya (Clostridium) histolytica and Identifying novel structural features in HPT and REC domains from VarS histidine kinase in V. alginolyticus
… isolation pancreatic islets. </p> <p>For V. alginolyticus, the VarS/VarA two-component systems is responsible for regulating the expression of collagenase. All two-component systems consist of a extracellular sensing domain, a catalytic and ATP binding (CA) domain, a dimerization and …
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Investigation of viscosity effects on bacteria collective motion using particle image velocimetry
… and behavior of Escherichia coli and Vibrio alginolyticus. Firstly, various PIV post-processing tools are examined; next, different PIV conditions are tested and post-processing methods are optimized to best visualize bacteria collective motion; finally, the influence of viscosity contrast on …
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Wirkung des Ballastwasser-Biozids Acrolein auf verschiedene pathogene Bakterienstämme in Meer-, Brack- und Süßwasser
… hydrophila, Vibrio parahaemolyticus, Vibrio alginolyticus und zwei Stämme Vibrio vulnificus. Basierend auf der ScanMIC-Methode (Rahman et al, 2003) wurden Mikrotiterplatten mit Bakterienflüssigkultur, Acrolein und Probenwasser unterschiedlichen Salzgehaltes belegt, mit dem Farbstoff TTC …
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Exploring the optimality of various bacterial motility strategies : a stochastic hybrid systems approach
… strategy of the marine bacterium Vibrio alginolyticus. We use stochastic stability theory to analyze the chemotaxis models in terms of their stationary distributions and also derive a diffusion approximation of the system that provides further insight into the performance of various …
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Physical ecology of marine microbes
… adaptation in the marine bacterium Vibrio alginolyticus using high-speed imaging. We found that marine bacteria can exploit a buckling instability of their flagellum to change direction during swimming, achieving the same functionality as multi-flagellated cells, but with the cost of …
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Biogenic Amine Analysis of Fresh and Stored Bluefish (Pomatomus Saltatrix) and Microbiological Survey of Histamine-Forming Bacte
… isolate each of Klebsiella ozaenae and Vibrio alginolyticus, and two isolates of Aeromonas sp. were found in the processing facilities. The study concluded that histamine-forming bacteria do not make up a large part of the microflora associated with fish-processing facilities. Fishing vessels …
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Molecular characterisation of the vibrio midae SY9 extra cellular alkaline serine protease and its role in the previously observed probiotic effect on the growth of Haliotis midae
… VmproA, which has high similarity to a Vibrio alginolyticus extracellular detergent resistant alkaline serine protease. Furthermore, during the course of this investigation it became apparent that VmproA may represent an extracellular alkaline detergent-stable, member of the proteinase K-like …