Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Alexa Fluor"”.
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Frequency domain fluorescent molecular tomography and molecular probes for small animal imaging
… thesis, we have developed a frequency domain fluorescent molecular tomographic system based on the heterodyne technique using a single source and detector pair for small animal imaging. In our system, the intensity of laser source is modulated to produce a diffuse photon density wave in the …
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Development of a TIRF Microscope for Single Molecule Binding Studies
A single-molecule total internal reflection fluorescence microscope (TIRFM) is necessary to study the crucial interaction between PCNA and CAF-1 in replication-coupled nucleosome assembly to contribute to epigenetic inheritance. The microscope measures fluorescence following total internal …
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Generation of Glioblastoma specific SNAP based antibody fusion proteins for future radiolabelling application
… the SNAP-tag element was tested using BGmodified fluorophores. To characterize highly specific in vitro labelling, the SNAP-tag-based fusion proteins were conjugated to BG-Alexa Fluor 647 to generate an immunofluorescent protein that can label TAAs in live tumour cells. To characterize the …
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The Effects Of Clinorotation In The Host Plant, Medicago Truncatula, And Its Microbial Symbionts
… Image J. AMF colonization was quantified using Alexa Fluor 488 and the magnified intersection method. Clinorotation significantly reduced M. truncatula dry mass and fresh mass compared to the static controls. The addition of rhizobia treatments under clinorotation altered total root length and …
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Engineering Fluorescently Labeled Human Fibroblast Growth Factor One Mutants and Characterizing Their Photophysics Properties Towards Designing FRET Assays
… we successfully designed and characterized fluorescently labeled hFGF1 tracers which span the visible light region of the electromagnetic spectrum for use in smFRET. Fluorescent labeling was achieved by utilizing the maleimide moiety of the dyes (Alexa Fluor, Cyanine and iFluor) to conjugate …
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Extending the utility of enzymes for site-specific targeting of fluorescent probes
Genetically encodable fluorescence reporters such as the green fluorescent protein (GFP) are useful for studying protein expression, localization, and dynamics in a variety of biological systems. GFP and its related variants, however, suffer from several drawbacks. Compared to chemical …
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Cavity coupled photonic crystal enhanced fluorescence for high sensitivity biomarker detection
With the rise in fluorescence-based testing of biomarkers in samples, the use of dielectric optical resonators has increased dramatically. These photonic crystals make fluorophores emit more light through a process called enhanced fluorescence which in turn allows for more signal to be collected …
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Dynamics and Turnover of Lipid and Protein Domains in Cells and Supported Bilayers
… substrate interacts with commonly used red fluorescent dye (Alexa Fluor 594), as a result Fibronectin is used for further studies. Single molecule dynamics of Syx1a at clusters using a novel system of nanobody interacting with enhanced green fluorescent protein (EGFP) labeled Syx1a reveals …
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Cellular delivery and site-specific targeting of organic fluorophores for super-resolution imaging in living cells
Recent advances in super-resolution fluorescence microscopy have pushed the spatial resolution of biological imaging down to a few nanometers. The key element to the development of such imaging modality is synthetic organic fluorophores with suitable brightness and photostability. However, organic …
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Development of SNAP-tag-based fusion proteins targeting HIV-1 viral reservoirs
… cell line. Cell-surface binding of SNAP-Surface® Alexa Fluor® 488 -conjugated J3-SNAP on Env expressing HEK293Tcells was assessed by confocal microscopy analysis. Results Successful expression of J3-SNAP in E. coli BL21-DE3 was confirmed by SDS-PAGE and Western blot analysis. The J3-SNAP fusion …
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Design and Development of Nanoconjugates for Nanotechnology
… which are their abilities to serve as donors in fluorescence energy transfer (FRET) and surface energy transfer (SET) as well as to do multiplexing, to engineer QD-based nanoconjugates for optical and biomedical applications. Immobilizing luminescent semiconductor CdSe/ZnS QDs to a solid platform …
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Ratiometric fluorescent metal ion indicators based on functionalized poly(N -isopropylacrylamide)
<p>A novel type of ratiometric fluorescent indicator based on the phase transition of a well-known stimuli-responsive polymer poly(N-isopropylacrylamide) (PNIPAM) is presented. The sensing mechanism involves the polymer conformational change resulting from charge neutralization by the analyte metal …
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Amphiphilic Cyclic Cell-Penetrating Peptides Containing Tryptophan and Arginine as Anticancer Agents and Drug Delivery System
… and confocal microscopy confirmed the uptake of Alexa-Fluor siRNA (AF-488 siRNA) in the presence of [WR]9 at different concentrations. The calculated binding affinity (BC50) of siRNA to the peptide was 1.9, indicating strong binding for siRNA to the peptide. As a result, the peptide siRNA …
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Multiplexed Photometry And Fluorimetry Using Multiple Frequency Channels
… </p> <p> <strong>MULTIPLEXED PHOTOMETRY AND FLUORIMETRY USING MULTIPLE FREQUENCY CHANNELS</strong> </p> <p>by</p> <p> <strong>KHALED M. DADESH</strong> </p> <p>August 2013</p> <p> <strong>Advisor</strong>: Dr. Amar Basu</p> <p> <strong>Major</strong>: Electrical Engineering</p> <p> …
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Discovery of prognostic biomarkers from the prostate cancer membrane proteome using bioorthogonal chemistry
… detected by NeutrAvidinTM DyLightTM 650 (NA650) fluorescence using flow cytometry. Azidosugar labelling of glycoproteins was detected by Alkyne-Alexa Fluor 488 fluorescence using 1D gel electrophoresis, and Streptavidin 800 fluorescence using 2D gel electrophoresis. An increase in the overall …
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Pathological role of double-stranded DNA antibodies in multiple sclerosis.
… following tissue damage. Using novel DNA probes fluorescence suggestive of the presence of anti-dsDNA iii antibodies was seen in both animal and human tissue. Within human tissue the antibodies appeared to accumulate around active lesions and within vessels, raising the question of these …
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Pathological role of double-stranded DNA antibodies in multiple sclerosis.
… following tissue damage. Using novel DNA probes fluorescence suggestive of the presence of anti-dsDNA iii antibodies was seen in both animal and human tissue. Within human tissue the antibodies appeared to accumulate around active lesions and within vessels, raising the question of these …
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The effect of voluntary exercise on adult hippocampal neurogenesis in maternally separated rats
… detected in rat brains using t he Click-iT EdU Alexa Fluor 488 detection kit. Three molecular marker combinations were used to detect different factors for both dorsal and ventral hippocampi: (1) EdU/GFAP/NeuN, to establish how many EdU labelled cells survive to become neurons or astroc ytes (2) …
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Biochemical and Immunological Mechanisms Underlying Differential Interaction of Superantigens with Host Immunogenetic Factors in Streptococcal Sepsis
… was a correlation between binding affinity of Alexa fluor 647-labeled SAgs to individual class II alleles and response levels, except for SmeZ1 binding to and presentation by DQ alleles, where an inverse correlation was observed. However, Ca flux studies demonstrated good correlation with the …
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Development of SNAP-tag based fusion proteins as novel auristatin F-containing immunoconjugates and photoimmunotheranostics in the detection and treatment of triple-negative breast cancer
… on their molecular weights. Integration of the fluorescent dye Alexa Fluor 488 into the fusion proteins was carried out to investigate the self-labeling activity of the SNAP-tag moiety, as well as to provide qualitative and quantitative insights into the binding potential of the antibody …
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