Global ETD Search
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Showing 1 to 11 of 11 for “"Alcohol Oxidase"”.
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Characterization of the Pichia pastoris alcohol oxidase I promoter
… energy is attributed to the highly induceable Alcohol Oxidase I promoter (AOXI). Despite of the disperse popularity and use of this promoter over the last 15 years, little is known about the transcription controls at a molecular level. A 5'>3' deletion analysis of the AOXI promoter was …
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Aromatic alcohol oxidase, aldehyde oxidase and mannitol oxidase in terrestrial gastropods
Several unusual hydrogen peroxide generating oxidase activities have been investigated in the alimentary tissues of terrestrial gastropod molluscs. A membrane-bound aromatic alcohol oxidase activity has been demonstrated in the digestive gland (hepatopancreas) of four families of gastropod …
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Evaluation of immobilizing horseradish peroxidase and alcohol oxidase in PVA-AWP polymer
… on the activity and stability of horseradish peroxidase (HRP) and alcohol oxidase (AOX) when immobilized in a photo-crosslinkable and water-soluble polymer, poly (vinyl alcohol) azide-unit water pendant (PVA-AWP). The immobilized enzyme films were to be used in biosensing applications and their …
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Characterization of the 5̕ untranslated region ( 5̕ UTR) of the alcohol oxidase I (AOX I) gene in Pichia pastoris.
… nucleotide 5' Untranslated Region (UTR) of the Alcohol Oxidase I (AOXI) gene in Pichia pastoris. The 5' UTR influences the expression of many heterologous proteins in P. pastoris. However, no systematic analysis has ever been performed on this region to date. Several truncated versions of the 5' …
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Exploring Protein-Ligand Interaction with Rotamer-Library Based Refinement Tools
… binding protein, truncated hemoglobins and aryl alcohol oxidase. In the latter two cases, the pathways were identified as part of studies including quantum mechanical calculations and experimental results. The structure prediction methods, coupled with experiments, allowed the quantum methods to …
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Proteomic insights into the marine obligate hydrocarbonoclastic bacteria (OHCB) during alkane degradation
… not only biosynthesised a cytochrome P450, an alcohol oxidase and an alcohol dehydrogenase but also expressed a set of enzymes for β-oxidation of the resultant fatty acids generated during hydrocarbon degradation, overcoming potential steric hindrance. The mechanisms O. antarctica utilises to …
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Calcium homeostasis in Pichia pastoris
… growth medium did not increase the expression of alcohol oxidase-1 driven β-galactosidase expression. However, calcium and magnesium may still play crucial roles in protein expression as EDTA and EGTA caused an increase in β-galactosidase expression as indicated by higher β-galactosidase activity. …
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Detection of hydrogen peroxide and ethanol in exhaled breath using biosensors: Considerations in standardizing breath collection
… (SPCEs) were used solely or immobilized with alcohol oxidase as biosensors for detecting the chosen biomarkers amperometrically. Akaike's information criterion, Bayesian information criterion, and cross validation were adopted in predictive model selections, and uncertainty analyses were …
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Upgrading alcohols with bio-oxidation and chemo-catalysis in single-flask processes
… processes. In this vein, our group targets alcohol substrates for upgrading with biocompatible chemistry. Alcohols are attractive targets for their easy access from renewable feedstocks yet scarce opportunities for upgrading in water. Since alcohols are relatively inert towards traditional …
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Designing and optimizing tandem bio- and chemo-catalyzed cascade reactions to access industrially relevant chemicals in mild, aqueous conditions
… system capable of converting short chain primary alcohols to α,β-unsaturated aldehydes in aqueous conditions. Alcohol substrates are oxidized to the corresponding aldehyde by the biocatalyst, whole cell Gluconobacter oxidans (G. oxidans), followed by an organocatalyzed aldol addition-condensation …
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Design and Engineering of Carbon Fixing Material Systems
… hydrogen peroxide generation with the enzyme alcohol oxidase. Finally, modeling was performed to understand carbon nanotube-based sensors and chemically-responsive materials, which may find use in the detection and quantification of greenhouse gas emission streams and the abatement of such …