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Showing 1 to 19 of 19 for “"Alcian blue"”.
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Aportaciones al conocimiento del proceso de glicosilación en levaduras: Aislamiento y caracterización de mutantes de Saccharomyces Cerevisiae con defectos en dicho proceso
… una baja afinidad por el colorante básico "alcian blue" y por esta razón los llamamos mutantes Idb (baja unión de colorante).
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Regeneration of Hair Cell Epithelia in the Chick and Salamander
… salamander to regulate this regeneration. Alcian blue staining was used to compare the extracellular matrix of normal and regenerating neruomasts. Alcian blue staining at two different pHs can distinguish between highly sulfated and non-sulfated forms of glycosaminoglycans and …
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Osteogenic and chondrogenic differentiation of rat bone marrow stromal cells in 3D culture with novel PCL-TCP scaffolds
… chondrogenic differentiation was confirmed by alcian blue staining and toluidine blue staining. In conclusion, it was demonstrated that PCL-TCP scaffolds with fibrin glue facilitate proliferation, anchorage, motility, and differentiation of both specialized cells and BMSCs.
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Sequência de ossificação craniana de Aparasphenodon brunoi Miranda-Ribeiro, 1920, com comentários sobre o condrocrânio e o desenvolvimento da ornamentação óssea (Amphibia, Anura, Hylidae)
… of tadpoles, froglets and juveniles, cleared and alcian blue - alizarin stained. The animals were staged using Gosner (1960) for tadpoles, and the extension and degree of cranial ornamentation for froglets and juveniles. The tadpole, the chondrocranium at different developmental stages, the …
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Gastric remnant carcinoma : histochemical and immunohistochemical profile
… stage. In this study haematoxylin and eosin, alcian blue pH 2.5, periodic acid Schiff, high iron diamine and Giemsa stains as well as immunohistochemical methods (eight antibodies against MUCI to MUC6) were used to determine the type of mucin and the pattern of staining in twenty cases of GRC …
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Surface enhanced resonance Raman spectroscopy of dyes: semi-quantitative trace analysis
… and alizarin. For some dye molecules e.g. alcian blue it was possible to combine the linear regions of normal (non-resonance/non-enhanced) Raman and SERS/SERRS plots, thereby extending the dynamic range available for semi-quantitative analysis. The sensitivity of the SERS/SERRS technique …
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A study of the functional anatomy of the bovine cervix with special reference to the epithelium, mucus secretion and sperm transport
… from each quarter cervix were stained with (1) Alcian blue (AB) at pH 1.0 (sulfomucins) and at pH 2.5 (sialomucins and sulfomucins); (2) periodic acid Schiff (PAS) (neutral mucins) and AB (pH 2.5); (4) high iron diamine (HID) (neutral and sulfomucins) with AB (pH 2.5). Additional samples were …
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The Misexpression of Sonic Hedgehog Leads to Digit Duplication
… a mutant mouse line that mis-expresses Shh. Alcian blue staining of cartilaginous tissue shows that these mutant mice exhibit polydactyly and other related skeletal defects. Differences in gene expression between mutant and control mice were assessed using in-situ hybridization with probes …
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MyosinX is Required For Craniofacial Development in Danio Rerio
… development using various staining techniques. Alcian blue staining was used to identify specific defects within the cartilage, specifically ceratobranchial arches 3-5 are distorted or completely missing in <em>myosinX</em> deficient embryos. Using alizarin red staining techniques, pharyngeal …
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The Isolation and Differentiation of Adipose Derived Mesenchymal Stem Cells
… produced cells capable of adipogenesis. Yet, alcian blue staining demonstrated that sonication was unable to yield cells capable of chondrogenisis. Lastly, rat adipose-derived stromal cells were cultured in medium containing stimulators of UCP1 expression in order to induce a brite cell …
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Pathogenic effects of Mycoplasma hyopneumoniae on porcine tracheal organ cultures and neutrophils in vitro, and porcine respiratory goblet cells in vivo
… of lung sections stained with high iron diamine/Alcian blue. Goblet cells in bronchi of infected pigs contained significantly less total mucin and sialomucin, and significantly more sulphomucin than goblet cells of age matched control pigs. Increased sulphated mucin may indicate altered …
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Systematics of the macrourid fishes
… result of the method of preparation. In rattails alcian blue reveals hyaline cell cartilage at the margins of certain dermal elements where it is gradually replaced by bone.
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Anatomical study of the variation in the branching patterns and histology of the aorta in a South African population
… sample were stained with haematoxylin and eosin, alcian blue pH2.5 - periodic Schiff reaction and elastin von Gieson’s for histological analysis using a Zeiss Axioskop Mot upright microscope and Axiovision 4.7 software. Variation in the branching pattern of vessels along the length of the aorta …
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Συμβολή εις την μελέτην των χορδωμάτων
… intercellular mucous substance was positive with Alcian Blue in 100% of the cases. The cells in cases did not show either significant atypia or mitotic activity. The histologic picture of one case which gave metastases was similar with the histologic picture of the other cases which did not give …
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The role of folic acid supplementation on exencephaly induced by maternal exposure to sodium valproate in the TO mouse
… malformations and hemorrhage. Alizarin red and alcian blue stained skeletal preparations disclosed the maxillary and mandibular hypoplasia, absence of skull vault, hemivertebrae, longitudinal fusion of the vertebral arches and bodies, accessory ribs (cervical and lumbar), fusion of thoracic …
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The Decelluarized Human Umbilical Vein (HUV) as an Allogeneic Scaffold for Vocal Fold Tissue Engineering
… staining with hematoxylin and eosin, Alcian blue with and without hyaluronidase, Periodic acid-Schiff's reagent, Safranin-O, and Masson's Trichrome were used to examine cellular attachment, depth of cellular infiltration, the structure of the tissues and the scaffolds, and the …
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The Decelluarized Human Umbilical Vein (HUV) as an Allogeneic Scaffold for Vocal Fold Tissue Engineering
… staining with hematoxylin and eosin, Alcian blue with and without hyaluronidase, Periodic acid-Schiff's reagent, Safranin-O, and Masson's Trichrome were used to examine cellular attachment, depth of cellular infiltration, the structure of the tissues and the scaffolds, and the …
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Tissue engineering of a tracheal substitute
… tissue engineering within the scaffold. Alcian blue (AB) and Papanicolau (PN) stains of the tissue engineered cartilage described two distinct regions within the constructs, namely the developed cartilage-like region and the developing region. The latter is thought to be areas in which …