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Showing 1 to 20 of 26 for “"Alcaligenes"”.
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MOLECULAR CHARACTERIZATION AND EXPRESSION OF GENTISATE 1,2-DIOXYGENASE FROM PSEUDOMONAS ALCALIGENES NCIB 9867
… mutagenesis of the gene cloned from Pseudomonas alcaligenes NCIB 9867. Alignment of known GDO sequences indicated the presence of a conserved central core region containing a two-motif signature of the cupin superfamily, designated motifs 1 and 2. Mutations generated within this central core …
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Characterization of Reduced Phosphorus Metabolism in Alcaligenes Faecalis WM2072 and Xanthobacter Flavus WM2814
… identified by 16s rDNA analysis as a strain of Alcaligenes faecalis and a strain of Xanthobacter flavus. The genes required for oxidation of hypophosphite and phosphite by these organisms were identified using transposon mutagenesis and directed mutagenesis experiments. For A. faecalis these …
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Microbial Synthesis of Polymers: Alcaligenes Eutrophus and the Production of Poly - B - 3 - hydroxybutyric Acid (PHB)
PHB formation in bacteria is a means to store reserves of energy and carbon. The location and discovery of PHB has been known since 1926. In the last 30 years, much attention has been given to it, due to the polymer's characteristics. It is a thermoplastic polyester, the first truly biodegradable …
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Identification and characterisation of bacterial genes associated with resistance to and/or degradation of environmental pollutants
… with Polycyclic Aromatic Hydrocarbons (PAHs). Alcaligenes sp. AO22 and Arthrobacter sp. E9 were identified from the lead contaminated site, while Stenotrophomonas maltophilia was identified from the PAH contaminated site. Minimum Inhibitory Concentration assays (MICs) were previously performed …
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The role of enteric bacteria in the Abalone, Haliotis Midae
… tests. The common genera identified were Vibrio, Alcaligenes, Flavobacteria, Pseudomonas and Aeromonas. The bacterial communities in each gut region varied, suggesting that both resident and transient bacterial populations are present in H. midae. Alcaligenes occurred mainly in the crop, while …
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Herstellung von monoklonalen Antikörpern gegen die Cuabhängige dissimilatorische Nitritreduktase
… In einem zweiten Ansatz wurde die Cu-dNIR aus Alcaligenes faecalis S6 nach Fusion mit einem 6 x His-tag in E. coli exprimiert und anschließend über eine spezifische Affinitätsmatrix aufgereinigt. Hiermit konnte eine Ausbeute von 4,5 mg/l [2,25 mg/g Zellen (Feuchtgewicht)] Bakterienkultur …
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Biodegradation of tertiary butyl alcohol by an introduced Pseudomonas sp. in subsurface soil microcosms
… in subsurface soil microcosms. A TBA-degrading Alcaligenes sp. was isolated from the Fredrick clay. Limited TBA degradation was observed in one of three deep subsurface coastal plain soils after 220 days of incubation.
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The 16S rRNA characterization of a novel "microaerophilic" Pseudomonas sp. from the oligotrophic deep subsurface environment
… type strain, 10% homologous to <i>Pseudomonas alcaligenes</i>, and 5% homologous to <i>Pseudomonas aeruginosa</i>. Data from biochemical tests confirm the hypothesis that strain MR 100 is a novel species of <i>Pseudomonas</I. It was able to accumulate poly-β-hydroxybutyrate intracellularly, …
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Comparison of gold extraction yields by cyanide treatment vs. bioleaching procedure
… were leached using NaCN and native bacteria, <em>Alcaligenes faecalis,</em> in column experiments to compare the gold recovery yields in the effluents. Four columns were treated with a different liquid: water, bacteria, NaCN and a mixture of cyanide and bacteria. After they were leached the …
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Synthesis and application of a grafted flocculant produced from a chemical combination of a bioflocculant TKT and acrylamide (AM)
… a grafted bioflocculant produced from marine Alcaligenes faecalis HCB2 to wastewater. Extraction and purification of the bioflocculant was obtained through using the solvent extraction method. Chains of polyacrylamide (PAM) were grafted onto the backbone of bioflocculant TKT extracted from …
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Alicaligenes faecalis : identification and study of its antagonistic properties against Botrytis cinerea
… properties it was identified as a strain of Alcaligenes (aeca/is Castellani and Chalmers. Antisera prepared in Balbc mice injected with live and heat-killed bacterial cells gave strong reactions with the homologous immunogen and with ATCC 15554, the type strain of A. taeca/is, but not with …
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Regioselective synthesis of curdlan derivatives
… of beta-D-glucan, is produced by the bacterium Alcaligenes faecalis var. myxogenes. Several strategies to synthesize chemically modified curdlan derivatives have been reported, but there have been few reports of regioselective functionalization at specific positions of the curdlan backbone, …
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Antibacterial, antioxidant and phytochemical investigation of Acacia Arenaria, Aloe Esculenta and Pechuel-Loeschea Leubnitziae
… 8100), Enterococcus faecalis (ATCC 7080) and Alcaligenes faecalis (ATCC 8750). The minimum inhibitory concentration (MIC) of the extracts against afore mentioned microbes were determined. The antioxidant activity of the extracts was determined using α, α-diphenyl-β-picrylhydrazyl (DPPH) …
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Antibacterial, antioxidant and phytochemical investigation of Albizia Anthelmintica leaves, roots and stem bark
… show activity against Enterococcus faecalis and alcaligenes faecalis. The results from the study show that Albizia anthelmintica extracts possess compounds with antibacterial as well as anti-oxidative properties, making this plant species a good source for potent antibacterial and anti-oxidative …
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Solution properties and molecular size of polyhydroxybutyrate from recombinant Escherichia coli
… DH5a/pAeT41, containing the native operon from Alcaligenes eutrophus encoding the three enzymes necessary for the biosynthesis of PHB and DH5a/pSP2, a strain genetically engineered to optimize synthase production in E. coli. In the latter strain, the induction agent …
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Crystal structure of a human U5 snRNP specific binary complex and crystal structure of a histone deacetylase-like bacterial amidohydrolase
… ermittelt werden. Dieses Enzym aus Bordetella/Alcaligenes FB188 ist ein Histondeacetylase (HDAC)-Klasse 2 Homolog und stellt die erste Struktur eines HDAC-Klasse 2 Enzyms dar. Im allgemeinen sind HDAC s für die Prozessierung von Acetylresten an e-Aminogruppen von Lysinen an den Aminotermini von …
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Bioaugmentation with metal-resistance microorganisms in the remediation of metal and organic contaminated soils
… of 2,4-D by the cadmium-sensitive 2,4-D degrader Alcaligenes eutrophus JMP134 in the presence of toxic levels of cadmium. Ina pilot field study, a cadmium-detoxifying Pseudomonas isolate enhanced 2,4-D degradation by A. eutrophus JMP 13 4 in the presence of cadmium.
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