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Showing 1 to 11 of 11 for “"Alanine substitution"”.
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Evaluating the Interaction Between the Human Melanocortin-2 Receptor and the Accessory Protein, Mrap1: Chimeric Receptor and Alanine Substitution Studies on Transmembrane Domain 4, Extracellular Loop 2, and Transmembrane Domain 5
… utilized a chimeric protein paradigm as well as alanine substitution experiments to observe potential contact sites between MRAP1 and the MC2R. By using these approaches, important residues required for trafficking or activation were identified in transmembrane 4, extracellular loop 2, and …
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Molecular characterization of a sphingomyelin binding site in the vacuolating cytotoxin of H. pylori
… cause substantial attenuation in toxin activity, alanine substitution of 2 tryptophan residues within the putative binding site led to significant attenuation. Further, tryptophan to alanine mutation of W603, a residue present in the center of the putative binding site, was found to be attenuated …
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Role of region 4 of the sigma 70 subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing
… element. The effect of 16 single and 2 triple alanine substitution variants of sigma 70 between amino acid residues 590 and 613, was determined in vivo by measuring the rate of transcription from a luxI-lacZ translational fusion via b-galactosidase assays in recombinant E. coli. In vitro work …
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CRISPR/CAS9 mediated gene editing for the improvement of beef and dairy cattle
… CRISPR/Cas9 system to induce single-base pair substitutions, thereby improving animal genetics through removal of undesirable or insertion of beneficial mutations. In Angus beef cattle (Bos taurus), a mutation in the NHL-repeat containing 2 gene causes a heritable abnormality referred to as …
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Role of the C-terminal domain of the <font face = "symbol">a</font> subunit of RNA polymerase in transcriptional activation of the <i>lux</i> operon during quorum sensing
… has been investigated. The effect of seventy alanine substitution variants of the <font face = "symbol">a</font> subunit was determined <i>in vivo</i> by measuring the rate of transcription of the <i>lux</i> operon via luciferase assays in recombinant <i>Escherichia coli</i>. The mutant RNAPs …
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Mutations in Phospholamban Alter the Structure and Function of the Calcium ATPase Regulatory Complex
… SERCA. We then studied the effect of C-terminal alanine substitutions and truncation mutations on PLB oligomerization and SERCA regulation by fluorescence resonance energy transfer (FRET) measurements in live cells. In addition, we also studied the structural and functional consequences of two …
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Synthesis and mass spectrometry studies of oligopeptides
… of model N- and C-terminal cysteine polyalanine peptides in which one L-alanine was substituted by a D-alanine viz. CAA<sup>D</sup>A and AA<sup>D</sup>AC, were measured by the extended Cooks kinetic method. Gas-phase acidities of CAA<sup>D</sup>A and AA<sup>D</sup>AC were measured to be …
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Genetic and biochemical characterization of YrkF, a novel two-domain sulfurtransferase in Bacillus subtilis
… YrkF<sup>C15A</sup>, containing a cysteine to alanine substitution in the Ccd1 domain was created to determine if the Ccd1 cysteine is essential for rhodanese activity. The variant protein was overexpressed and rhodanese assays showed that YrkF<sup>C15A</sup> is also a functional rhodanese. …
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Escherichia coli 2-oxoglutarate dehydrogenase multienzyme complex: e1 and e2 substrate specificty, e1 carboligase activity, and e2 interchain succinyl transfer
… pathway. The E2oLD and E2o with a lysine to alanine substitution at position 43 (E2oK43A) were created by site directed mutagenesis. It is clearly shown that E2oLD was capable of rescuing the crippled E2oK43A variant by measuring the NADH production in the overall reaction. Therefore, an …
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Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing
… procedures have been used to generate alanine-substitution mutants in the C-terminal forty-one amino acid residues of LuxR, a region that has been hypothesized to play a critical role in the activation process. An <I>in vivo</I> luminescence assay was first used to test the effects of …
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The Impact of Candida albicans Glycogen on Fitness, Virulence and Host Response
… binding proteins in various species and alanine substitution of each residue in this motif significantly impaired glycogen accumulation in C. albicans. Moreover, fluorescent protein tagging, microscopy, and co-immunoprecipitation (Co-IP) indicated that C1_01140Cp colocalized and …