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Showing 1 to 12 of 12 for “"Alanine Scanning Mutagenesis"”.
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Recombinant Expression of Preptin Analogs for Alanine Scanning Mutagenesis of Residues 27-29
… insulin-independent diabetes and osteoporosis. Alanine scanning mutagenesis was utilized to substitute an alanine for residues Trp<sup>27</sup>, Arg<sup>28</sup>, and Gln<sup>29</sup> of preptin in order to ultimately study the structure-activity relationship of these residues to preptin’s …
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Design, Cloning, and Expression of Rat Preptin and Alanine Analogs
<p>Alanine scanning mutagenesis was employed to determine the structural epitopes of preptin; a newly discovered polypeptide found on the proinsulin-like growth factor II. The alanine scanning mutagenesis was accomplished by the use of, cloning, affinity chromatography, gel electrophoresis, gene …
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Antigen Recognition by an Alloreactive T Lymphocyte Clone
… is mapped using soluble 2C TCR and the alanine-scanning mutagenesis technique. A majority of the binding energy for alloantigen recognition is derived from residues within the CDR 1 and 2 regions of the 2C TCR. Additional TCR mutants are analyzed in chapter five, including several which …
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Molecular characterization and evolutionary plasticity of protein-protein interfaces
… for small-molecule therapeutics. However, alanine-scanning mutagenesis studies have revealed that only a subset of residues contribute the greater part of free energy to binding - so-called "hot-spots". Molecular characterization of hot-spots performed using PICCOLO, probes the molecular …
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Using Esterase and Laccase Enzymes to Derivatize Bioactive Plant Phenolics for Altered Chemistry
… inactivation during lyophilization. Still, alanine scanning mutagenesis revealed that R37A substitution improved activity of AF1753 on long-chain p-nitrophenyl (pNP) esters. A multicopper oxidase (SCO6712) from Streptomyces coelicolor displayed activity on a variety of phenolics including …
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Functional Domains in the Multigene Regulator of the Group A Streptococcus
… family of putative virulence gene regulators. Alanine scanning mutagenesis demonstrated the importance of CMD-1 for transcriptional activation in several members of this family. This established that the results yielded from investigations of Mga could be broadened beyond the GAS to provide …
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The Role of Transmembrane Domain Helix-Helix Interactions in the Function of Pentameric Ligand-Gated Ion Channels
… between M4 and M1/M3 influence channel activity. Alanine scanning mutagenesis of this interface shows that disruption of almost any interaction in GLIC leads to a loss of folding and/or function, while analogous mutations in ELIC typically lead to no change or produce gains in function. Sequence …
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Long peptides for cysteine arylation
… as its structural and mechanistic properties. Alanine scanning mutagenesis revealed mutations that diminished reactivity in addition to others that improved its function. Computational structure prediction suggested a mixed helical and random coil structure. Combining the beneficial mutations …
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Transcriptional Control during Quorum Sensing by LuxR and LuxR Homologues
… (RNAP). Published work describing the results of alanine scanning mutagenesis performed on the C-terminal domain of LuxR (residues 190-250) has identified residues (K198, W201 and I206) that appear to play a role in positive control of transcription initiation. Additional mutagenesis of residues …
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Structural and biophysical analysis of Human DNA repair protein CtIP
… of its interaction with double-strand DNA. Using alanine scanning mutagenesis, I have investigated the zinc-binding ability of putative metal-coordination residues within the C-terminal domain. Furthermore, I provide evidence for the presence of a coiled-coil region N-terminally juxtaposed to the …
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Polyphosphate and tissue factor/factor VIIA as initiators of coagulation
… of TF mediate the effect of Mg2+, I performed alanine scanning mutagenesis and substrate activation experiments, identifying several key residues mediating these effects. Importantly, these residues reside in or near the putative substrate binding exosite, suggesting that Mg2+ occupation of GLA …
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In search of catalytic proficiency: The importance of enzyme conformational change to orotidine 5’-monophosphate decarboxylase catalysis
… problem, kcat/KM = 1.3 x 107 M-1s-1 (2). Through alanine-scanning mutagenesis, I have identified important residues in OMPDC catalysis (3, 4). The large impact of mutating residues on the periphery of the active-site has helped develop an understanding of the importance of conformational change. …