Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"Air-liquid interface"”.
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Development and characterisation of air-liquid interface exposure systems for cell and tissue cultures
… cell culture models that did not capture air-liquid interactions, identifying the need for more comprehensive in vitro aerosol exposure systems to examine how aerosolised substances impact respiratory tissues, cellular responses, toxicity, and overall health. Aerosols possess opposing …
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Mechanisms of virus infection of human airway epithelium cultured at an air-liquid interface (HAE-ALI)
ABSTRACT Polarized human airway epithelial (HAE) cultures are instrumental for studying respiratory tract diseases and gene delivery of airways. Primary epithelial cells isolated from tracheobronchial airways, grown in transwell at an air-liquid interface (ALI), undergo media-induced mucociliary …
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Maturation of pluripotent stem cell-derived alveolar type II cells at air-liquid interface and response to environmental stimuli
… during expiration. AT2s exist at a physiological interface between inhaled air and pulmonary vasculature, and are among the pulmonary cell types that are directly exposed to inhaled environmental stimuli such as respiratory viruses and cigarette smoke. Primary human AT2s can be cultured in vitro …
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Understanding the eco-evolutionary dynamics underpinning changes in air-liquid interface biofilms in radiating populations and multi-species communities
Static liquid microcosms have been used to study evolutionary and ecological dynamics of bacterial populations, where metabolic activity produces a heterogenous environment with a low-O2 region in the liquid column, and a high-O2 region directly below the air-liquid (A-L) interface. This system has …
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Structural and kinetic studies of surfactant and phospholipid monolayers at the air/liquid interface using sum-frequency generation spectroscopy
… surfactant and phospholipid systems at different interfaces. Firstly, the effects of temperature and ionic strength on the adsorption of cationic and non-ionic surfactants were investigated. The binary surfactant system used here is a simplified hair conditioner model, consisting of a fatty …
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Developing bioelectronic sensors for 3D in vitro models at the air liquid interface: Innovative technology for biomedical and respiratory research.
Respiratory diseases and airborne infections are significant global health issues and a major cause of morbidity and mortality worldwide. According to the World Health Organization (WHO), respiratory diseases rank as the third leading cause of death globally, with over 10 million deaths per year …
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The SmartLab: experimental and environmental control and monitoring of the chemistry laboratory
… of many components of an experiment studying the air/liquid interface using the technique of second harmonic generation (SHG). Software will be described that can be used to controls actuators, laser sources and other hardware within the laboratory. This software will use the same message broker …
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Advanced Bioelectronic and Microphysiological Systems for Functional Studies of Stem Cell-Derived Neural Models
… microphysiological and bioelectronic systems; • Air-liquid interface cerebral organoids (ALI-COs), interfaced with Neuroweb, a porous, ultra-flexible microelectrode array enabling chronic electrophysiology; • NeuroMaps, a modular MATLAB-based GUI for multimodal electrophysiological analysis, …
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Cytokines and aeroallergens in the pathogenesis of summer-pasture associated obstructive pulmonary disease: effects on endothelin production, neutrophil activation and chemotaxis
… disease (SPAOPD), a naturally occurring airway disease of horses, is characterized by clinical exacerbation associated with exposure to pasture environment during the summer. Aeroallergens are believed to trigger exacerbation of SPAOPD, cytokines are likely associated with the anamnestic …
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Investigating the role of ZEB2 in the establishment of neuroepithelial architecture and axon tract formation
… aspects of the ZEB2 mutant phenotype we adapt air-liquid interface culture to cerebral organoids. Air-liquid interface cerebral organoids (ALI-COs) develop thick axon tracts with distinct morphologies and hodologies; including long-range projection within and away from the organoid, growth cone …
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Removal of Bacteria from Solids by Bubbles: Effect of Solid Wettability, Interaction Geometry, and Liquid–Vapor Interface Velocity
Air bubbles are a promising means of controlling fouling for a range of applications, particularly delaying fouling in marine environments. This work investigates the mechanism by which the collision of an air bubble with a solid removes adsorbed bacteria. A key feature of the work is that the …
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Free methionine sulfoxide reductase and its contributions to environmental stress response in Komagataeibacter hansenii
… bacterium that forms cellulosic biofilms at the air-liquid interface of liquid media, serving as a model for biofilm forming pathogenic bacteria. In this thesis, the role of free methionine-R-sulfoxide reductase (MsrC) was investigated through comparative analysis of the wildtype (WT) K. …
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IMPACTS OF TROPICAL PEAT-FOREST SMOKE: URBAN PM2.5 AND TOXICITY IMPLICATIONS
… burning in the Maritime Continent on regional air quality and public health, this thesis characterizes chemical composition of urban PM2.5. Urban PM2.5 are systematically classified to smoke-dominant (SD) vs. non-SD samples. In-situ pH increases from 1.9 in non-SD PM2.5 to 2.0 and 2.7 in SD and …
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Staphylococcus aureus의 Lipoteichoic acid(LTA)에 의한 사람의 정상 코 점막 상피세포에서의 MMP와 TIMP의 발현
… 분리한 상피세포 배양 1. 사람 코 점막 상피세포의 Primary culture 2. Air Liquid Interface-culture(ALI-culture) B. Lipoteichoic acid(LTA) 처리 C. MTT assay D. RNA 추출 및 cDNA 합성, PCR E. Enzyme-linked immunosorbent assay(ELISA) F. Gelatin zymography 1. Gelatinase B의 준비, 활성화 2. Gelatinase B의 전기영동, 기질-효소 간 반응 3. 발현된 band의 …
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Sequence-Specific Gene Correction of Cystic Fibrosis Airway Basal Cells
… correction with an efficiency of ~10 % in CF airway basal cells homozygous for the ΔF508 mutation. Basal cells are a multipotent stem cell population of the respiratory epithelium and therefore, their gene correction could provide a long-term, permanent remedy for CF. Delivery of engineered …
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Applications of eScience to interfacial chemistry
… harmonic generation at the air/liquid interface was examined and tools produced<br/>to aid the process. All stages of the process are considered, the experimental stage, the<br/>data workup and publication of results.<br/><br/>Specifically covered were tools for remote …
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In vitro models for airway epithelial cell culture
… of a physiologically relevant model of the human airway. Various factors such as the cell model, physiochemical factors such as the cell substrate properties including its stiffness, shear stress, stretch, the air-liquid interface and the biochemical factors in the medium influence the biology of …
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Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease
… delivery of different of more toxic drugs to the airways in order to increase efficiency of both the delivery mechanism and of the drug utilised. Here we looked at the use of a number of different mechanisms, which can be used as stand-alone devices/delivery agents and/or in conjunction with other …
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Bacterial collectives
… they readily evolve mat‑forming lineages at the air‑liquid interface in static microcosms. Although beneficial in the short term, these mats lack intrinsic group‑level reproduction. Their incomplete multicellularity makes them ideal for studying early steps toward multicellularity. In this …
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