Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Agrobacterium-mediated transformation"”.
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Agrobacterium-Mediated Transformation of Soybean: Test of Selectable Marker Genes
… traditional soybean breeding approaches. Soybean transformation still remains a great challenge. To increase soybean transformation efficiency, three selectable marker genes, bar, ALS and ASA2 , were tested with two soybean transformation systems, e.g. cotyledonary nodes and mature embryo axes …
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Improved regeneration and Agrobacterium-mediated transformation of wild strawberry (Fragaria vesca L.)
… work was to develop an efficient high-throughput Agrobacterium-mediated transformation protocol to generate an insertional mutant population to support the justification of F. vesca as a model organism for rosaceous crops. The transformation techniques described by Alsheikh et al. (2002) and …
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Adeno-associated virus rep78 expression in Arabidopsis thaliana
… alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana via Agrobacterium-mediated transformation. PCR and sequence analysis …
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Regeneration, DNA Methylation, and Transformation in Rosaceae
… containing pCAMBIA 2301 plasmid was used for transformation. A transformation protocol by taking advantage of a secondary somatic embryogenesis system was developed. Then, a microbial protein gene, Ace-AMP1, was introduced into Rosa hybrida cv. Carefree Beauty via Agrobacterium-mediated …
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Agrobacterium-mediated gene transfer into kiwifruit
… been developed to aid in the establishment of Agrobacterium-mediated transformation for new plant species. A series of binary vectors have been constructed that express a chimaeric β-D-glucuronidase (GUS) gene in plants cells but not in bacterial cells. This feature allows GUS activity from …
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Genetic Engineering of Black Cherry (Prunus serotina) for Reproductive Sterility and Insect Pest Resistance
… The goal of this project was to optimize the transformation and in vitro rooting system, and to develop transgenic black cherry for reproductive sterility and insect pest resistance.</p> <p>To achieve this, a TERMINAL FLOWER 1 homolog from black cherry was isolated and characterized as one of …
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Tamarillo mosaic potyvirus: characterization and resistance
… in three different binary vectors suitable for Agrobacterium-mediated transformation of plants. Of the twenty-six independent Nicotiana benthamiana plant lines generated expressing either TaMV coat protein or TaMV RNA sequences modified to block translation, eight plant lines demonstrated …
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Analysis of AtNHX1-expressing transgenic cotton under high salt conditions and in the field
… Arabidopsis gene AtNHX1 into cotton by using the Agrobacterium-mediated transformation. The AtNHX1-expressing cotton plants produced more biomass and fibers when grown in the presence of 200 mM NaCl in greenhouse conditions. The increased fiber yield was likely due to better photosynthetic …
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The use of transgenic tobacco as a production and delivery system for a vaccine against hemorrhagic enteritis virus of turkeys
… promoter (hmg2) on plasmids adapted for Agrobacterium-mediated transformation. Approximately sixty transgenic plants of each construct were generated and determined to contain the HEV fiber gene based on amplification of specific HEV DNA sequences by the polymerase chain reaction. Plants …
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Genetic engineering of sorghum and switchgrass for improved biofuel production
… study is to optimize sorghum regeneration and transformation processes using standard binary vectors and bar gene as a plant selectable marker. The optimized transformation process enables reproducibly to achieve over 14% transformation frequency, the highest transformation efficiency through …
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Analysis of ABA-regulated expression of the maize Glb1 gene in tobacco seeds and maize cells
… vector and introduced into tobacco plants by Agrobacterium-mediated transformation. Histochemical GUS assays of R$\sb{\rm o}$ tobacco mature seeds indicate that the Glb1 promoter drives GUS expression in ABA treated seeds, but not in water treated ones. Further GUS assays of the R$\sb1$ seeds …
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Improving barley for biofuel production – investigating the role of 4CL and CCR in the lignin biosynthesis pathway
… 4CL1 and CCR1 using a constitutive promoter via Agrobacterium-mediated transformation of barley. From an extensive screen of the primary transformants for changes in protein level and lignin content, six CCR and four 4CL lines were taken forward for detailed analysis. Antibodies were also raised …
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In Vitro Regeneration and Genetic Transformation of Sesbania Drummondii: A Medicinally and Environmentally Important Plant
… pCAMBIA 1305.1, was used to develop a genetic transformation system for S. drummondii. This plasmid contains a GUS (P-glucuronidase) reporter gene and a gene conferring resistance to the antibiotic hygromycin. The genes are placed under the control of the 35S CaMV (cauliflower mosaic virus) …
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Attempts toward the expression in transgenic Arabidopsis thaliana of Esi47, a salt stress-induced gene encoding a protein kinase from Lophopyrum elongatum
Plant transformation vectors for constitutive gene expression were prepared to study the role of Esi47, a gene that encodes a protein kinase, which normally is induced as part of the salt-stress response in Lophopyrum elongatum. Agrobacterium-mediated transformation of A. thaliana, by …
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Regeneration and Transformation of Impatiens walleriana Using Cotyledonary Node Culture
… generating transgenic plants involves the use of Agrobacterium-mediated gene transfer. A commonly used technique involves transformation of explant tissue and subsequent regeneration in vitro under aseptic conditions. However, prior to our research there was no regeneration protocol suitable for …
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Membrane Domain of Plant 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase: Targeting, Topology, and Function
… a FLAG epitope to facilitate in vivo studies. Agrobacterium-mediated transformation was used to introduce the tagged hmg1 gene into two Nicotiana tabacum cell lines, BY-2 and KY-14. The slow growth kinetics of KY-14 prevented effective recovery of transformed lines, however, Northern analyses …
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Genetic engineering for pest resistance in potato (Solanum tuberosum L.)
… of interest into the plant genome consists of Agrobacterium-mediated gene transfer. This thesis studied Agrobacterium-mediated transformation to transform two potato cultivars with an avidin gene anticipated to confer insecticidal activity against certain lepidopteran insects. A successful gene …
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NAC transcription factors in sugarcane and their functional role in drought tolerance
… stress. ShNAC23 was introduced into tobacco via Agrobacterium-mediated transformation, and physiological and biochemical parameters were evaluated under water-deficit conditions. Transgenic plants displayed enhanced early growth vigour, but drought responses did not mirror the protective effects …
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The Animal-fungi Hybrid Cell Cycle of the Zoosporic Fungus Spizellomyces punctatus - a New Model to Understand Evolution of Eukaryotic Cell Cycle Control
… from MNase-seq data.</p><p>Finally, I developed Agrobacterium-mediated transformation in Spizellomyces, allowing me to describe basic characteristics of its developmental program using live-cell and fluorescence microscopy. By following nuclear dynamics with a fluorescently tagged histone I found …
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Development of a genetic transformation system in the family Ceratocystidaceae with specific reference to Ceratocystis albifundus
The usefulness of transformation systems in fungal pathogens has been well described. Although functional studies in the Ceratocystidaceae is lagging behind model fungi from Aspergillus and Neurospora, the economic impact of many species in this family on both the agricultural and forestry …
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