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Showing 1 to 20 of 84 for “"Agrobacterium tumefaciens"”.
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Analysis of factors affecting the virulence of Agrobacterium tumefaciens
The Ti plasmid of Agrobacterium tumefaciens T37 was reconstructed in several different plasmids. The performance of the border repeats alone and in conjunction with overdrive and a left border inhibitory region was measured in the potato disc assay. These results were compared to T-strand …
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Characterization of the VtlR regulons in Brucella abortus and Agrobacterium tumefaciens
Brucella abortus and Agrobacterium tumefaciens are pathogenic bacteria that infect animals and plants, respectively. These bacteria are genetically similar and are found within the same Class, Alphaproteobacteria, and Order, Rhizobiales, of the domain Eubacteria; however, they survive and replicate …
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Analyse von Protein-Protein Interaktionen im Typ IV Sekretionssystem von Agrobacterium tumefaciens
Agrobacterium tumefaciens ist ein Gram-negatives Bodenbakterium, das dikotyle Pflanzen infiziert. Mittels eines Typ IV Sekretionssystems werden dabei ein Teil der bakteriellen DNA (T-DNA), sowie die Virulenzproteine VirE2, VirE3, VirD2 und VirF in die pflanzliche Zelle transferiert. Das Typ IV …
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PopZ and FtsZ coordinate polar growth termination and cell division in Agrobacterium tumefaciens
… wall synthesis in the bacterial plant pathogen, Agrobacterium tumefaciens. Since cell wall (peptidoglycan) biosynthesis during elongation and cell division is vital for bacterial survival, we expected many key proteins involved in these processes to be essential for cell survival. Thus, we …
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Characterization of The Agrobacterium Tumefaciens Virb2 Pilin of The Virb/D4 Type Iv Secretion System
The <em>Agrobacterium</em> <em>tumefaciens</em> VirB/D4 type IV secretion system (T4SS) delivers oncogenic T-DNA and effector proteins to susceptible plant cells. This leads to the formation of tumors termed Crown Galls. The VirB/D4 T4SS is comprised of 12 subunits (VirB1 to VirB11 and VirD4), …
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Molecular, genetic, and biochemical studies of mannityl opine utilization by Agrobacterium tumefaciens: Its evolutionary implications
Agrobacterium strain NT1 harboring pSaB4, which contains the 14-kb BamHI fragment 4 from the octopine/mannityl opine-type Ti plasmid pTi15955, grows well with agropine (AGR). However, this strain grows slowly with mannopine (MOP) as sole carbon source, but gives rise to spontaneous mutants which …
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The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation
The transfer of T-DNA from Agrobacterium tumefaciens to eukaryotic cells is on of the most extensively studied examples of interkingdom DNA transfer. This transfer system has revolutionized plant molecular biology to the extent that the introduction of foreign genes into plant genomes is now a …
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Effect of krilium on the respiratory activities of Rhizobium trifolii and Agrobacterium tumefaciens on various substrates
The respiratory rates of Rhizobium trifolii and Agrobacterium tumefaciens were measured by use of a Warburg Respirometer. A non-proliferating or "resting cell" technique was utilized in demonstrating the effect of Krilium on the oxygen consumption of the organisms when Krilium was aided to a given …
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Characterization of Theacc (Agrocinopine Catabolism) Locus on the Nopaline-Type Ti Plasmid From Agrobacterium Tumefaciens Strain C58
… elements located in the promoter region of accR. Agrobacterium strains were attracted to several opines tested. The determinants for chemotaxis to these opines were localized to opine catabolic regions of the Ti plasmids. Analysis of insertion mutations and subclones of acc showed that accA, which …
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Construction of inducible expression systems for controlled transfer of Agrobacterium tumefaciens T-DNA in plant transient expression systems /
… produced in plants in contained facilities using Agrobacterium-mediated transient transfection of plants. However, transfection methods suitable for open field applications are still desirable as a cheaper alternative. Biosafety concerns related to the use of recombinant agrobacteria in an …
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Molecular, Genetic and Physiological Studies on the Regulation of Conjugal Transfer of the Ti Plasmid pTiC58 by Agrobacterium Tumefaciens
Conjugation assays determined that the bacteria mate in a cell density dependent manner, known as quorum-sensing. Analysis of transfer frequencies using a transfer-constitutive mutant of pTiC58 showed that quorum sensing requires both the opine induction and autoinduction systems. Analysis of lacZ …
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Molecular Genetic Characterization of the Mating Pair Formation and Vegetative Replication Regions on the Agrobacterium Tumefaciens Ti Plasmid pTiC58
… traI, is responsible for the synthesis of Agrobacterium autoinducer (AAI) which is the co-inducer of the transcriptional activator, TraR. The next 11 ORFs showed similarities with the trb genes of RP4, which encode mating pair formation (Mpf) functions essential for conjugation, as well as …
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Molecular genetic characterization of essential conjugal transfer determinants on the nopaline-type Ti plasmid from Agrobacterium tumefaciens strain C58
Ti plasmids of Agrobacterium tumefaciens encode a conjugal transfer system. I characterized a region, called tra, that is necessary for transfer of the nopaline-type Ti plasmid, pTiC58. tra contains the origin of conjugal transfer (oriT) and a cluster of genes the products of which are predicted to …
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Elucidating the roles of the diguanylate cyclases CelR and avmA in attachment and cellulose synthesis with the response regulator divK in Agrobacterium tumefaciens
Agrobacterium tumefaciens, the causative agent of crown gall disease in plants, physically interacts with plant cells to remain anchored within the nutrient-rich rhizosphere. Part of this interaction involves the production of cellulose fibrils by the bacteria, leading to the aggregation of the …
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Soybean transformation and analysis of transformed tissues using PCR
… which affect the transformation efficiency of Agrobacterium tumefaciens-mediated soybean transformation were studied. It was found that pre-induction of A. tumefaciens A281 using acetosyringone significantly increased the efficiency of transformation and this could be boosted further by …
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Expression of Gus Gene in Tobacco (Nicotiana tabacum) and Corn (Zea mays) Using Dimboa-Resistant Agrobacterium Strains
<p>This thesis evaluates the ability of Agrobacterium tumefaciens strains resistant to the compound DIMBOA [2, 4-dihydroxy-7-methoxy-2h-l,4- benzoxazin-3(4H)-one] to infect corn. DIMBOA is a secondary plant metabolite and is believed to have antimicrobial properties and provides resistance to the …
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Characterization and Expression of Plant Defense Genes in Peach and Tomato
… cv. 'Sweet Chelsea' were cocultivated with Agrobacterium tumefaciens strain GV3101 harboring derivatives if the binary vector pCAMBIA 2301. The binary vectors pGR-1, pChi-1, pRIP-1, and pAFP-1 carried a maize beta-glucanase, maize chitinase, iris ribosome-inactivating protein (RIP), or an …
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An investigation of the molecular biology and genetics of Witches' Broom disease of Protea cynaroides
… but these were unsuccessful. Four strains of Agrobacterium tumefaciens were unable to induce tumours on P. cynaroides seedlings. Sections of vascular tissue from teratoma and healthy tissue were viewed under the electron microscope but revealed no pathogen.
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