Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Ago2"”.
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Dynamic Shuttling of AGO2 to the Nucleus Relieves MicroRNA Repression
… enrichment of core RISC effector protein, AGO2. We tested the consequences of nuclear AGO2 localization on global microRNA regulation using a multipronged transcriptomics approach. We combined AGO2-eCLIP-sequencing, small-RNA-sequencing, and whole transcriptome sequencing to identify …
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AGO2 in overexpression exhibits oncogenic functions KrasG̳1̳2̳D̳ -associated mouse tumor models
… novel oncogenic functions associated with AGO2 overexpression. In short, we have determined that AGO2 overexpression promotes metastasis in an autochthonous mouse model of non-small cell lung cancer while elevated AGO2 levels in B cells contribute to the initiation and maintenance of …
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Egfr Modulates Microrna Maturation In Response to Hypoxia Through Phosphorylation of Argonaute2
… stress through phosphorylation of Argonaute2 (Ago2) at Tyr393. The association between EGFR and Ago2 is enhanced by hypoxia, leading to elevated Ago2-Y393 phosphorylation which in turn reduces the binding of Dicer to Ago2 and inhibits miRNA processing from precursor miRNAs (pre-miRNAs) to …
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MicroRNA-184: A Multifaceted Regulator of Epidermal Keratinocyte Biology
… modulates the expression of Argonaute 2 (AGO2), an established effector of miRNA function. However, there is a paucity of research investigating miR-184 and the miR-184:AGO2 axis in epidermal keratinocyte biology. As such, the aim of this thesis was to delineate miR-184 expression and …
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Exploring the specificity and mechanisms of siRNA-mediated gene silencing in mammalian cells
… is catalyzed by the endonuclease Argonaute 2 (Ago2), knockdown of Ago2 expression did not affect mRNA knockdown for imperfectly complementary combinations. These results indicate that off-target mRNA reductions are likely attributable to Ago2-independent degradation processes. Using the same …
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Genetic and molecular analysis of <em>drop out</em>, the single homolog of the vertebrate MAST kinases in <em>Drosophila melanogaster</em>
… an allele of the RNA silencing gene argonaute2 (ago2). However, results presented in this thesis showed that ago2 functions are unimpaired in dop mutant embryos using genetic and biochemical tools. Moreover genetic and molecular mapping revealed that dop mutants carry a mutation in a gene within …
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Molecular requirements for FMRP and RNA helicase MOV10 in the translational regulation of co-bound mRNAs
… comparing CLIP binding sites of FMRP, MOV10, and AGO2 in the 3' UTR of co-bound mRNAs. All three proteins exhibit an enrichment of binding proximal to commonly known microRNA Recognition Elements (MRE) sites in HEK293 cells. However, we also observed that in a subset of mRNAs, FMRP and MOV10 …
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Development and characterization of chemical modifications for siRNA strand selection
… effector of RNAi is an RNA-guided nuclease, Ago2, which selects one of the two strands as a guide to form the RNA induced silencing complex (RISC). Incorporation of the antisense, rather than the sense, strand is crucial for potency and safety siRNA therapeutics. Thermodynamic stability, …
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Caractérisation des voies de synthèse de petits arns non codants en réponse à divers stress chez le doryphore de la pomme de terre
… significative des niveaux de transcrits d’Ago2 dans les doryphores exposés à 40 °C par rapport aux témoins. D’autre part, toutes les cibles, y compris Ago2, ont montré la même réponse aux quatre températures testées pendant l’exposition au froid. Pour les insectes traités avec des …
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Functional analysis of the Argonaute family proteins during early embryonic and germ cell development in the mouse
… mammals, genetic experiments revealed that Ago2 is the central effector for both siRNA and miRNA silencing pathways in several developmental processes. Phosphorylation of serine 388 of murine protein is dependent on the MK2 and Akt/PKB pathways and regulates Ago2 localization. I created a …
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Regulatory Mechanism of the RNAi Pathway
… use of recombinant Drosophila Dicer-2, R2D2, and Ago2 proteins. We further employed this core reconstitution system to purify a RNAi regulator that we named C3PO (component 3 promoter of RISC), a complex of Translin and Trax. C3PO is a novel Mg2+ -dependent endoribonuclease that promotes RISC …
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Investigation sur la fonction du gène AGO5 dans le développement des plantes et la défense antivirale
… Dans l'interaction Arabidopsis-virus, AGO1, AGO2 et AGO4 sont parmi les protéines AGO les plus étudiées. Ceci est principalement dû à l'ordre dans lequel elles ont été identifiées, à leur implication dans de nombreux processus de développement ou à leur rôle dans la défense antivirale contre …
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Discovery of miRNA-regulated processes in mammalian development
… miRNA-targeted mRNAs by immunoprecipitation of Ago2 in the miRNA effector complex (miRNP). In one purification method, the Ago2-bound mRNAs are identified by microarray. The second purification method adds crosslinking, RNase digestion, and cloning and deep-sequencing (CLIP-seq) to improve the …
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RACE-Seq identifies the Argonaute-2 cleavage products of RNA interference-based oligonucleotides
… the Argonaute-2 RNA-induced silencing complex (AGO2-RISC) and directs the protein complex to homologous RNA. Cleavage of target RNA occurs opposite bases 10-11 when counting from the 5’ end of the hybridized siRNA guide strand. The capture and identification of these cleaved products by 5’ Rapid …
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Building the Human Argonaute 2 Interaction Network
… by which these proteins act. I have studied AGO2 interactions through immunoprecipitation and a novel semi-quantitative mass spectrometry technique known as the Normalized Spectral Index Method (SINQ). Stringent screening of mass spectrometry results identified interactions with the TRNC6 …
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Function and regulation of PARP13 binding to cellular RNA
… by inhibiting the activity of Argonaute 2 (Ago2), a member of the miRNA silencing pathway. While itself catalytically inactive, PARP13 is modified by PAR and can target Ago2 for modification by a yet unknown PARP. However, it remains unclear if RNA binding is required for this function of …
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Spin Shot Noise measurement
… charges, was predicted more than a century ago2 and has been an invaluable tool for examining the mechanism of charge transport and the nature of current-carrying excitations.3-8 A noise in a nonequilibrium angular momentum current analogous to shot noise seen in charge flow is expected, …
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Interactions between Cucumber mosaic virus proteins and host proteins
… aphids and stimulates resistance mechanisms by AGO2. The CMV 1a protein (a replicase component) is able to moderate antibiosis induction by the 2b VSR. This ensures that aphid vectors are deterred from feeding but not poisoned when they feed on CMV-infected Arabidopsis plants. I found that the …
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Nuclear binding and translation regulation by FMRP through novel associated protein and putative RNA helicase MOV10
… binding sites of MOV10. We also found that Ago2 binding sites influenced the fate of MOV10 clip targets, suggesting a role of MOV10 acting as a facilitator or inhibitor of miRNA mediated regulation depending on proximity to Ago2 binding sites. Chapter 4 develops a novel single molecule study …
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Characterizing global gene expression and antiviral response in Frankliniella occidentalis infected with Tomato spotted wilt virus
… thrips with dsRNAs of F. occidentalis Dcr-2 or AGO2 by hemocoel injection, and 3) construct transgenic plants expressing a thrips-gene specific dsRNA hairpin to target a vital gene. My research has catalogued insect response to TSWV activity in thrips during development and provides candidate …
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