Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"Agar plates"”.
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Salivary carriage of candida species in children and their mothers in a Western Cape population salivary carriage of candida species in children and their mothers in a Western Cape population
… Saliva samples were cultured on both blood agar and Sabouraud dextrose agar plates. Cultures were isolated, counted, and identified. Positively identified cocci were then isolated and identified as specific Streptococcus species using the API strep 20E system (Biomerieux). Positive yeasts …
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The Recovery and Transfer of Aerosolized Listeria Innocua
… (RH) levels (83% and 65%). Oxford Listeria agar plates, stainless steel coupons and polyethylene (HDPE) coupons in the chamber were exposed to the aerosolized bacteria for 5, 10, 20 or 40 minutes. Also, at these times, air samples (100 L) were collected on to gelatin filters which were …
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Airborne Campylobacter in a Poultry Processing Plant
… days and at 4 hour intervals onto Campy-Cefex agar plates or gelatin filters that were subsequently transferred to Campy-Cefex agar plates. Additionally, pre-evisceration and post-chill carcass rinses were analyzed quantitatively for Campylobacter. The mean level of airborne Campylobacter was 5 …
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Ein Beitrag zum Vorkommen von Yersinia enterocolitica in Hackfleisch und Fleischerzeugnissen vom Schwein im Hinblick auf die eingesetzten Kultur- und Isolierungsverfahren
… enterocolitica was isolated from selective CIN agar plates. Urea-positive colonies were identified using API 20E. Y. enterocolitica isolates were bio- and serotyped, and the pathogenicity was confirmed with Congo red binding and Calcium dependence on CRMOX agar plates. Pathogenic Y. …
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Isolation And Characterization Of Soil Microorganisms Capable Of Utilizing Used Motor Oil For Growth
… sole carbon-energy source were isolated from oil agar plates. These microorganisms were identified by either the 16S rRNA gene, or the 18S rRNA gene DNA sequences. Classical methods of identification were performed as a supplementation to the molecular method. The isolates were analyzed for their …
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Drosophila Melanogaster Oviposition and Toxicity Studies on a Chip
… chemical screening studies using agar plates are inaccurate, labor-intensive, and inflexible due to the manual chemical doping of agar. In this thesis, we have devised a miniaturization method to precisely and repeatedly manipulate agar stiffness and exposure area to quantitatively …
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Functionalization of CNFET arrays for chemical sensing
… the gas mixtures emitted from cultures grown on agar plates. This work highlights the potential of integrated gas sensors as a practical, rapid, and cost-effective approach for general gas sensing applications, including biomedical applications.
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Detection and Purification of a Novel Natural Inhibitory Compound from an Isolated Strain of <em>Rhodococcus</em> Using an Agar Extraction Method
… of secondary metabolites. The use of a solid agar extraction method to screen soil isolates of <em>Rhodococcus</em> for compounds with inhibitory activity against other bacteria resulted in the discovery of a promising candidate molecule. The <em>Rhodococcus</em> strain KCHXC3, isolated from …
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Operations capability improvement of a molecular biology laboratory in a high throughput genome sequencing center
… construction of DNA Libraries, and production of agar plates containing E.coli cell colonies (with many of copies of DNA). In this way, MPBG scales up raw DNA to a quality and quantity necessary for the subsequent high-volume, automated genome sequencing process. While most of the genome …
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A Lateral Root Defect in the wag1-1/wag2-1 Double Mutant of Arabidopsis
… a strong root waving phenotype on vertical hard agar plates only seen in wild-type roots when the seedlings are grown on inclined plates. Here an additional root phenotype in the wag1;wag2 mutant is reported. The wag1;wag2 double mutant displays both an increased total number and density of …
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A study of Door Pulls on Isolation Carts in a General Hospital for Evidence of Contamination
… laboratory where they were innoculated on blood agar plates and in thio- glycolate broth. These were incubated at 37.5° Centigrade for seventy-two hours. Plates were examined daily for any bacterial growth. Any growth seen was restreaked on new blood agar plates. Smears were made on colonies on …
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A taxonomic study of the genus Campylobacter
… (SIM) medium, or on triple sugar iron (TSI) agar slants, hippurate hydrolysis, aerobic growth on agar plates, a requirement for H₂ or formate for microaerophilic growth or H₂ or formate and fumarate for anaerobic growth, alkaline phosphatase activity, and deoxyribonuclease (DNase) activity …
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Using blue light for bacterial inactivation
… this, dilutions were made and exposed, on agar plates, to blue light (BL, 405 nm) or UV-C light (290 nm - 100 nm ) in a variety of experiments. Results. The results indicated that bacterial inactivation by BL is dose-dependent, the presence of a plasmid encoding UV-resistance genes did not …
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Density gradient procedures for the selection of synchronous cells of Synechococcus lividus and Chlorella sorokiniana, and the application of the isopycnic technique to the study of the patterns of phosphoribosylglycinamide synthetase during the cell cycle of Chlorella
… contaminating bacteria by selecting colonies off agar plates which had been maintained in an atmosphere of 4% CO₂-air. Attempts were made to synchronize the algae by intermittent illumination, isopycnic centrifugation, and differential centrifugation. Cells selected by differential centrifugation …
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Use of an Inducible Promoter to Characterize Type IV Pili Homologues in Clostridium perfringens
… wildtype when <i>C. perfringens</i> was grown on agar plates, and the PilA3 mutant lacked a von Willebrand factor A domain-containing protein in its secretome. We used our promoter system to express GFP-tagged versions of the TFP ATPase homologues and view them in cells growing on surfaces. We saw …
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Sensitive cross section of a bacterium, Escherichia coli, to fast neutrons
… all were opened aseptically, plated on agar, and colonies counted. Because of the extreme variation between colony counts of lyophilized samples, a weighted least squares analysis was performed to estimate the sensitive area. The total flux to which the bacteria were subjected was …
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Caldolase: an alkaline serine protease from Thermus strain TOK₃
… (particularly chelating agents) in casein agar plates, the second the inclusion of a variety of native and chromogenic proteins in the agar plates. In conjunction, these methods provided a basis for screening extreme thermophiles for particular proteases, and enabled the identification of …
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A microbiological and molecular study of campylobacter and related species isolated from ostriches (Struthio camelus)
… filtration onto antibiotic-free Tryptose blood agar plates, and incubation at 37 ºC in a hydrogen-enhanced microaerobic atmosphere (Lastovica, 2006). The isolates were identified as C. jejuni subsp. jejuni. The multiplex PCR of Neubauer and Hess (2006) was applied to some of these isolates. A …
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF LEGIONELLA BACTERIA USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION
… 10 days to grow <em>Legionella </em>colonies on agar plates. There are few rapid assays available that can identify multiple <em>Legionella </em>species. This project aims to develop a Loop Mediated Isothermal Amplification (LAMP) assay that can detect multiple species of <em>Legionella </em>in …
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Endophytic Actinomycetes as potential agents to control common scab of potatoes
… by spot inoculating endophytes and overlaying agar with the pathogen. Multiple layer agar plates were used to test the paired interactions among endophyte isolates on their ability to use quorum sensing to enhance antibiotic production to inhibit the pathogen. In each assay zones of inhibition …
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