Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"Affinity probes"”.
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Synthesis of bespoke matrices to investigate a novel anti-tumour molecular target using affinity chromatography. The design, synthesis and evaluation of biotinylated biarylheterocycles used as novel affinity probes in the identification of anti-tumour molecular targets.
… activity was independent of duplex DNA binding affinity. The compounds were tested in the NCI 60-cell line panel and COMPARE analysis suggests they have a novel mechanism of action, targeting the product of a ¿gene-like sequence¿ of unidentified function. The identity of likely protein targets …
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A fragment-based approach towards understanding molecular recognition of 4’-phosphopantetheine adenylyltransferase from Mycobacterium abscessus
… rational elaboration of fragments into higher affinity probes of Mabs PPAT. A combination of synthetic chemistry and biophysical methods were employed to elaborate hits into higher quality ligands and understand their interaction with Mabs PPAT. Chapters 2 and 3 describe the development of two …
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Aptamers that bind filovirus GP : a new frontier for detection and neutralization of Ebola and Marburg virus
Aptamers are single chained, nucleic acid-based affinity probes that bind to their targets with strong affinity and specificity. They are made through an in vitro combinatorial selection method, wherein large libraries of nucleic acids with randomized sequences are subjected to an iterative process …
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Ligandability of protein-protein interactions and surfaces on Cullin RING E3 ubiquitin ligases
… binding to VBC and the development into high-affinity probes.<br/><br/>The findings of this work provide structural and biophysical contributions into the whole CRL2VHL complex assembly and functioning and provide insights and tools that could aid future targeting of this CRL.
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Antimalarial Agents: New Mechanisms of Actions for Old and New Drugs
… After this observation, a number of photo-affinity probes were designed and synthesized in hopes of isolating the molecular target of MQ. These analogs are currently being used by Dr. Klemba in pull-down experiments. In the third project, conducted in collaboration with Professor Belen …