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Showing 1 to 20 of 214 for “"Affinity chromatography"”.
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Advances in DNA Affinity Chromatography
<p>Different aspects of DNA affinity chromatography such as DNA complexity heparin elution, the Bi-column method and the oligonucluotide trapping method were studied. The complexity (length) of a DNA sequence attached to an affinity chromatography column affects column retention, and the purity of …
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Antibody Purification from Tobacco by Protein A Affinity Chromatography
… the isolation and purification of antibodies. An affinity chromatography purification step utilizing Protein A resin introduced early in the purification process can reduce successive unit operations, thereby reducing the overall process cost. However, directly applying tobacco extract to Protein …
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Endotoxin removal from protein solutions by immobilized metal ion affinity chromatography
… from protein solutions, immobilized metal (ion) affinity chromatography (IMAC) has been developed in this study. Fe^{3+} was charged to a chelating gel that contains iminodiacetic acid (IDA). The IDA- Fe^{3+} IMAC column was exploited to remove endotoxin from protein solutions. This method …
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Preparation and characterization of Staphylococcal protein A adsorbents for high performance affinity chromatography
… aspects of the application of High Performance Affinity Chromatography (HPAC) to bioanalysis are addressed. The initial study demonstrates the quantitative determination of IgG from serum using HPAC. Columns were prepared by immobilizing Protein A from Staphylococcus aureus on 10 m diol-bonded …
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Downstream Processing with Affinity Chromatography. A Study of a Continuous Process for Biospecific Adsorption
… Although the adsorbent displayed sufficient affinity towards trypsin, the overall adsorption performance was not satisfactory. Subsequent studies were carried out with a p-aminobenzamidine silica adsorbent, which gave far superior results. Amounts of up to 35 mg of trypsin per g of silica …
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Isolation of a human milk sialyloligosaccharide by affinity chromatography with wheat germ agglutinin (WGA)
Lectin affinity chromatography has been applied to the separation of the sialyloligosaccharides of human milk. A human milk sialyloligosaccharide fraction was tritium labeled and applied to a highly substituted WGA-agarose column (20 mg/ml). Only a single component from the complete …
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Isolation of immunoglobulins from cheese whey and milk using ultrafiltration and immobilized metal affinity chromatography
… whey was applied to an immobilized metal affinity chromatography (IMAC) column to isolate IgG. With a 50 ml IMAC column loaded with 17.3 ml of 50 mM CuCl₂ to get 2/3 Cu saturation, 1240 mg of IgG could be isolated with 56% purity. About 152 mg of lactoferrin and 90 mg of lactoperoxidase …
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Detection and enrichment of cytochrome P450s using bespoke affinity chromatography and proteomic techniques. Development of chemical immobilisation and novel affinity chromatography methods, with subsequent proteomic analysis, for the characterisation of cytochrome P450s important in cancer research.
… standardised work-flow was created, encompassing affinity chromatography, protein concentration/desalting, followed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and high performance liquid chromatography-mass spectrometry (HPLC-MS). A ketoconazole analogue and a 2-EN …
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Characterisation of protein phosphorylation in Arabidopsis thaliana and Chlamydomonas reinhardtii based on affinity chromatography and mass spectrometry
… was developed. This method relies on the affinity of the phosphate group towards aluminum hydroxide and was termed MOAC (Metal Oxide Affinity Chromatography). Successful phosphoprotein enrichment even from highly complex protein mixtures was confirmed by separation of phosphorylated from …
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Proteomic Profiling of Pro and Active Matrix Metalloproteinases using Tandem Mass Spectrometry. Optimization of Affinity Chromatography and nHPLC-MALDI-MS/MS for Proteomic discrimination of Matrix Metalloproteinases in pre-clinical Cancer Model.
… a triazine dye-based ligand immobilized on chromatography beads was utilized to assess whether it binds to recombinant human MMPs (rhMMPs). The results highlighted that the ligand interacts with latent forms of MMPs in agreement with the literature. Secondly, the potential of the ligand was …
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Part One: Iodinated Estrogens - Potential Photoaffinity Labeling and Tumor Localizing Agents for Estrogen Responsive Tissues. Part Two: Synthesis of a Hexestrol Derivative for Potential Use in Affinity Chromatography to Purify Estrogen Receptors
Made available in DSpace on 2014-12-13T20:09:38Z (GMT). No. of bitstreams: 1 7606804.pdf: 4754811 bytes, checksum: 5e28e9583b4f5a10e05d7cff564649b7 (MD5) Previous issue date: 1975
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Synthesis of bespoke matrices to investigate a novel anti-tumour molecular target using affinity chromatography. The design, synthesis and evaluation of biotinylated biarylheterocycles used as novel affinity probes in the identification of anti-tumour molecular targets.
… activity was independent of duplex DNA binding affinity. The compounds were tested in the NCI 60-cell line panel and COMPARE analysis suggests they have a novel mechanism of action, targeting the product of a ¿gene-like sequence¿ of unidentified function. The identity of likely protein targets …
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Novel antibiotics from DNA adenine methyltransferase inhibitors
… bacteria, was expressed and purified by nickel affinity chromatography. A plasmid containing the methylation sensitive restriction endonuclease dpnI gene was assembled. DpnI was expressed and purified by nickel affinity chromatography. A Y. pestis Dam activity assay, which relied on the …
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The penicillin-binding proteins of Bacilli
… with non-ionic detergents and isolated by affinity chromatography. Prior separation of the PBPs by ion-exchange chromatography followed by affinity chromatography has. resulted in the purification of PBPs 1, 3 and 4 of B. megaterium. PBP5 has also been purified by affinity chromatography …
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Purification and characterization of a poly(dG).poly(dC)-binding protein from Parenchinus angulosis
… suGF1 was purified to near homogeneity by DNA-affinity chromatography. The purification procedure involved a cation exchange step, followed by poly(dG).poly(dC)-affinity chromatography, and finally by affinity chromatography utilizing multimerized specific DNA-binding sites of suGF1. The 59,5 …
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Proteomic Analysis of the Flavonoid Biosynthetic Machinery in Arabidopsis Thaliana
… mass spectrometry, with well-established affinity chromatography methods to further characterize the organization and composition of the Arabidopsis flavonoid enzyme complex. One of the key flavonoid enzymes, chalcone isomerase (CHI), was used in these experiments to detect interacting …
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Design, Cloning, and Expression of Rat Preptin and Alanine Analogs
… was accomplished by the use of, cloning, affinity chromatography, gel electrophoresis, gene sequencing, and mass spectra. However, due to inconclusive results, more research is required before the epitopes can be elucidated.</p>
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Identification and Function of Beta-1,4-Galactosyltransferase Associated Proteins
… phage display cDNA library screening and protein affinity chromatography were applied to identify GalTase-I associated proteins. Several proteins were identified as candidates that interact with GalTase-I and potentially play important roles during fertilization. Deciphering proteins that interact …
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Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT)
… of contaminating proteins by anion exchange chromatography (HGXPRT does not bind under the conditions used), followed by Reactive Red 120 agarose affinity chromatography.
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Glycolipids in mouse F9 teratocarcinoma cells: some changes associated with retinoic acid-induced differentiation
… by gel filtration and high performance liquid chromatography. These analyses indicated that differentiated F9 cells synthesized less high molecular weight oligosaccharides (containing more than 5 sugar residues) relative to controls. Serial lectin affinity chromatography on columns of …
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