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Showing 1 to 15 of 15 for “"Affinity Purification-Mass Spectrometry"”.
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Using affinity purification -- mass spectrometry to identify aryl hydrocarbon receptor nuclear translocator interacting proteins
… of this research is to use a combination of affinity chromatography and mass spectrometry techniques to identify the proteins that interact with ARNT.</p> <p>We chose mouse liver as the protein source. We planned to isolate the ARNT interacting proteins from the mouse liver protein extract by …
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Statistical methods to infer biological interactions
… to infer protein-protein interactions from affinity purification mass spectrometry (AP-MS) and from luminescence-based mammalian interactome mapping (LUMIER). Our work reveals novel context dependent interactions in the MAPK signaling pathway and insights into the protein homeostasis …
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CRISPR-based Transcriptional Activators for Dissecting Human Gene-Regulatory Mechanisms
… of the dCas9-toolbox, we showed that affinity-purification-mass spectrometry could be applied to identify the antagonistic gene for these dCas9-based tools in the cellular context, which enables us to improve dCas9-based transcriptional activation by targeted antagonist neutralization …
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Identification of Epstein-Barr virus proteins that inhibit the DNA damage response
… To identify the host interactions of BKRF4, an affinity purification-mass spectrometry experiment was performed and identified histones as the most prevalent interacting partner. In keeping with this finding, an in vitro binding assay performed with purified BKRF4 and purified histone octamers …
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Evaluating Chemical Crosslinking as a Tool for Enhancing DNA Damage Repair Interactome Analysis
… choice is not fully understood. Conceptually, Affinity Purification-Mass Spectrometry (AP-MS) methods can be used to determine the composition and subunit stoichiometry of such complexes. However, none of the existing AP-MS workflows combine isolation of complexes with high temporal sampling, …
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The Evolution of Ribosome Assembly: Making Ribosomes in the Mitochondria
… employing a combination of endogenous tagging, affinity purification, mass spectrometry, and cryo-electron microscopy, three distinct assembly intermediates (States 1-3) were successfully visualized at resolutions ranging from 3.2 to 3.8 Å, revealing the stepwise progression of mtSSU biogenesis. …
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THE HCMV INTERACTOME: A QUANTITATIVE ANALYSIS OF HUMAN CYTOMEGALOVIRUS-HOST PROTEIN INTERACTIONS
… are beyond the scope of these approaches. Affinity-purification mass spectrometry was performed to identify binding partners for 169 canonical, and 2 non-canonical HCMV proteins in infected cells. CompPass filtering determined an extensive network of high-confidence interacting proteins, …
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Identification of <i>Phytophthora capsici</i> nuclear effector targets to bolster host immune signalling and resistance pathways
… from Importin α1 results in higher binding affinity to CRN1_719, as well as its mislocalisation from the nucleolus. An affinity purification mass spectrometry approach aimed to better understand the role of Importin α1 and α2 upon infection showed that Importin α1 and α2 proteomes are …
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Diverse functions of LanC-like proteins in mammalian cells
… of C-glutathionylation in mammalian cells, affinity purification mass spectrometry-based (AP-MS) proteomic analysis was performed which is presented in Chapter 3. Based on the bioinformatic analysis of the proteomic data, it seems LanCL2 participates in different metabolic pathways as well …
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Mapping the metabolic protein interactome that supports energy conservation at the limits of life
… mapping the metabolic protein interactome using affinity purification mass spectrometry (AP-MS) and generating engineered strains where fusion proteins selectively redirect electron flux in vivo. Twenty-five proteins involved in distinct metabolic functions were tagged to reveal that each …
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An immunoproteomic approach to identifying cancer-associated autoantibody biomarkers
… CEACAM1, were also identified in this thesis by affinity purification-mass spectrometry (AP-MS) for patients from the same cohort, providing supporting evidence that these antigens are associated with CRC. The CT100plus microarray content was enzymatically modified to include citrullinated …
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Structure-function Analysis of the STRIPAK Complex
… subunits and scaffolds. In recent years, affinity purification coupled to mass spectrometry (AP-MS) has proven to be a powerful tool to identify protein-protein interactions (PPIs) involving kinases and phosphatases. Using AP-MS we discovered a novel-signaling complex, which we termed …
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An Activity-Based Proteomics Approach for Identifying Ajoene's S-thiolation Protein Targets in Blood and Cancer Cells
… differences in terms of tumour volume, mass and growth rate were observed compared to an untreated control. A follow-up study using a small library of eight ajoene derivatives varying ajoene's different functional groups in a blood stability study, revealed a proportional relationship …
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ENDOCYTIC CONTROL OF CELL-AUTONOMOUS AND NON-CELL-AUTONOMOUS FUNCTIONS OF P53
… the four major mammalian NUMB isoforms using an affinity purification-mass spectrometry approach. Among these interactomes, the endocytic adaptor protein Sorting Nexin 9 (SNX9) was found to be the most abundant specific interactor of NUMB-1 and NUMB-2. We validated this interaction through in …
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Proteomic Identification of Histone Post-Translational Modifications Induced By Dna Double-Strand Breaks and Novel Proteins Involved In The Dna Damage Response
… DSB-ChAP-MS (Double Strand Break-Chromatin Affinity Purification with Mass Spectrometry), for the affinity purification of a sequence-specific single copy endogenous chromosomal locus containing a DSB, followed by the proteomic identification of enriched proteins and histone PTMs. Providing …