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Showing 1 to 20 of 100 for “"Affinity Purification"”.
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Affinity purification of blood group A-active glycolipids on immobilized Helix pomatia lectin
Lectin affinity chromatography has proven to be a powerful method to separate oligosaccharides based on their stereochemical structures. This technique has not been used for the separation of glycolipids since mixtures of these compounds form micelles in aqueous solution. Since …
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Using affinity purification -- mass spectrometry to identify aryl hydrocarbon receptor nuclear translocator interacting proteins
… of this research is to use a combination of affinity chromatography and mass spectrometry techniques to identify the proteins that interact with ARNT.</p> <p>We chose mouse liver as the protein source. We planned to isolate the ARNT interacting proteins from the mouse liver protein extract by …
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Cytochrome AA(3) From Rhodobacter Sphaeroides: Affinity Purification and Biophysical Characterization of Site-Directed Mutants
… of R. sphaeroides has been purified using affinity methods and studied using site-directed mutagenesis coupled with a wide variety of biophysical characterization methods. Two methods of affinity purification were developed. Site-directed mutants were constructed in regions of the protein …
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Cytochrome AA(3) from Rhodobacter sphaeroides: Affinity purification and biophysical characterization of site-directed mutants
… of R. sphaeroides has been purified using affinity methods and studied using site-directed mutagenesis coupled with a wide variety of biophysical characterization methods. Two methods of affinity purification were developed. Site-directed mutants were constructed in regions of the protein …
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Monoclonal antibodies to bovine serum albumin : affinity purification and physicochemical characterization dc by Brian David Laffey.
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1995.
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Use of the cellulose-binding domain of a cellulase from cellulomonas fimi for affinity purification of fusion preteins
… domain (CBD) from a bacterial cellulase as an affinity tag for purification of heterologous proteins. The CBD of endoglucanase A (CenA) from Cellulomonas fimi is an N-terminal domain comprising amino acids. CenA binds strongly to cellulose, and the CBD retains this function when separated from …
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Study of the cell cycle interactome of Arabidopsis thaliana through a targeted proteomics approach
… and application of a high-througput tandem affinity purification (TAP)/mass spectrometry platform for cell suspension cultures to analyze protein complexes in Arabidopsis thaliana. The TAP technology was further optimized through the introduction of different alternative TAP tags. Finally, …
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Proteomic Identification of Histone Post-Translational Modifications Induced By Dna Double-Strand Breaks and Novel Proteins Involved In The Dna Damage Response
… DSB-ChAP-MS (Double Strand Break-Chromatin Affinity Purification with Mass Spectrometry), for the affinity purification of a sequence-specific single copy endogenous chromosomal locus containing a DSB, followed by the proteomic identification of enriched proteins and histone PTMs. Providing …
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Engineering streptavidin and its target ligands with both infinite binding affinity and reversible binding capability
… bioreactors, protein arrays, and matrices for affinity purification of proteins, it would be ideal for streptavidin to have extremely tight binding to its target ligands (biotin and its binding tags) and at the same time to retain the feature of reversible binding capability. To achieve this …
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Characterization of intracellular interactions between dengue virus and host proteins
… 38 out of 136 testable interactions using co-affinity purification assays from cultured cells. I tested each host protein against the proteins from all four serotypes of dengue virus and found that 57 out of 102 (56.9%) dengue-mosquito PPI and 34 out of 46 (73.9%) dengue-human PPI interacted …
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A flow cytometry-based method for gene expression profiling of CNS cell types
… to an existing technique, translating ribosome affinity purification, it was found that FANS was able to detect differentially expressed genes between two types of medium spiny neurons in the striatum with similar or higher sensitivity, using transgenic mice with fluorescence proteins labeling …
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Statistical methods to infer biological interactions
… to infer protein-protein interactions from affinity purification mass spectrometry (AP-MS) and from luminescence-based mammalian interactome mapping (LUMIER). Our work reveals novel context dependent interactions in the MAPK signaling pathway and insights into the protein homeostasis …
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Subunit Compositions of Arabidopsis DNA-Dependent RNA Polymerases and the Roles of the Plant-Specific RNA Polymerases IV and V in Gene Silencing
… and gene silencing. Using a combination of affinity purification and protein identification by mass spectrometry, my studies show that Arabidopsis Pol IV and Pol V are composed of subunits that are paralogous or identical to the twelve subunits of Pol II. Four subunits of Pol IV are distinct …
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FIV-FCA and FIV-PCO Reverse Transcriptases: Expression, Purification and Activity Comparison
… together by mixing induced cultures prior to affinity purification on a Ni2+ column. The heterodimer conformation of the enzyme was confirmed by the gel filtration and SDS gel electrophoresis. Both of the RTs were highly active in an in vitro RT assay. We compared the processivity of both RTs …
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FIV-FCA and FIV-PCO Reverse Transcriptases: Expression, Purification and Activity Comparison
… together by mixing induced cultures prior to affinity purification on a Ni2+ column. The heterodimer conformation of the enzyme was confirmed by the gel filtration and SDS gel electrophoresis. Both of the RTs were highly active in an in vitro RT assay. We compared the processivity of both RTs …
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Cavβ1a in the Nucleus of Muscle Progenitor Cells: Mechanisms, Functions, and Implications
… binding partners of CaVbeta1 were screened using affinity purification of CaVbeta1a-YFP from myoblast nuclear fractions coupled with mass spectrometry. Our results support the idea of CaVbeta subunits acting as transcription factors and regulating gene expression independently from CaV's, and …
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The TAZ Protein Interactome in Striated Muscle
… muscle cells. Using a novel GFP-Nanotrap based affinity purification approach coupled with LC-MS/MS protein identification, we document a comprehensive list of known and novel TAZ interactome components in myogenic cells. TAZ interacting proteins include components of Hippo signalling: TEAD1-4, …
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Roles of AMPK in Drosophila circadian clock
… β, γ subuit 간의 상호작용 확인 13 I. Tandem affinity purification (TAP) 15 J. 면역조직화학 (Immunohistochemistry) 16 Ⅲ. 결과 18 A. S2 세포에서 dCLK은 AMPK와 결합한다. 18 B. tim 발현 세포에서 AMPK의 발현이 저하된 초파리의 생체리듬 분석 21 C. AMPKα와 γ KD 초파리에서 AMPKα 단백질의 양이 감소하였다. 24 D. AICAR를 처리한 S2 세포에서 western …
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Functional Characterization of Atxa, The Bacillus Anthracis Virulence Regulator
… interacted non-specifically at a low nanomolar affinity with a target promoter (P<em>lef</em>) and AtxA-independent promoters. AtxA also contains motifs associated with phosphoenolpyruvate: sugar phosphotransferase system (PTS) regulation. These PTS-regulated domains, PRD1 and PRD2, are within …
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