Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Aeromonas hydrophila"”.
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Structural characterization of the type II secretion system of Aeromonas hydrophila
… exeC gene, found in the gram-negative bacteria Aeromonas hydrophila codes for a 31 kDa, three domain, bitopic inner membrane protein. The components of the ExeC protein include an amino-terminal cytoplasmic domain, a trans-membrane helix and two periplasmic domains. The two periplasmic domains …
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Isolation and Use of the Aeromonas hydrophila Secretin ExeD for Nanopore Analysis
… A larger pore, using the type II secretin of Aeromonas hydrophila, may be able to circumvent the size constraint of α-hemolysin and could allow research into larger molecules to be made. The goal of this project was to isolate and use the type II secretin ExeD in nanopore analysis. After this …
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Isolation, Characterisation and Preservation of Bacteriophages for Use in Treatment of Aeromonas hydrophila Infection In Finfish
… suitability of using lytic phages in controlling Aeromonas hydrophila infection in fish aquaculture. A. hydrophila is a fish pathogen and responsible for economic damages to the aquaculture industry. The main focus of this study was to develop and use a suitable model to test the efficacy of a …
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Mechanism of action of AexU, a new type III secretion system effector from an emerging human pathogen Aeromonas hydrophila
… system (T3SS) from a diarrheal isolate SSU of A. hydrophila. We identified an effector protein (designated as AexU) of the T3SS, which exhibited ADP-ribosyltransferase (ADPRT) and GTPase-activating protein (GAP) activity. AexU was successfully expressed in the HeLa cell Tet-Off system and I …
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Delineation of Aeromonas hydrophila Pathotypes by Dectection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay
<p><em>Aeromonas hydrophila </em>is a Gram-negative, bacterial pathogen of humans and other vertebrates. To delineate pathotypes, the genomes of twenty-eight <em>Aeromonas </em>isolates<em> </em>were screened by PCR to determine the presence of virulence factors including: aerolysin …
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Evidence of Water to Human Transmission of Aeromonas Hydrophila: Critical Role of Quorum Sensing in Bacterial Virulence & Host Response
Aeromonas hydrophila is a Gram-negative bacterium which produces a wide variety of virulence factors leading to both intestinal and extra-intestinal infections in humans. A large number of water and clinical Aeromonas isolates were molecularly finger-printed and our data provided the evidence of …
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The effect of temperature upon the growth and metabolism of aeromonas hydrophila and lactobacillus plantarum in pure and mixed culture
… and metabolism of pure and mixed populations of Aeromonas hydrophila and Lactobacillus p/an/Qrum were studied. Initially a medium was developed to provide unbiased support for both organisms. The effect of temperature upon lag phase, growth rate, and final population level between pure and mixed …
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Role of new virulence mechanisms/factors (type 3 secretion system and TOX-R regulated lipoprotein [TAGA]) in the pathogenesis of the emerging human pathogen Aeromonas hydrophila
Aeromonas hydrophila, a gram-negative bacterium that causes gastroenteritis, wound infections, septicemia, and other diseases in humans, produces many different virulence factors. A clinical isolate SSU of A. hydrophila possesses a cytotoxic enterotoxin Act, a potent virulence factor that is …
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Important role of the type VI secretion system in the virulence of an emergent human pathogen Aeromonas hydrophila: Delineating the mechanism of action of its effectors
Aeromonas hydrophila causes various human diseases, including gastroenteritis, wound infections and septicemia. In this study, we characterized the new type 6 secretion system (T6SS) from isolate SSU of A. hydrophila and demonstrated its role in bacterial virulence. We have provided evidence that …
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CHARACTERIZATION AND PATHOLOGICAL STUDY OF A. HYDROPHILA IN WALKING CATFISH (CLARIAS GARIEPINUS)
In this study, 18 strains of Aeromonas hydrophila had been isolated from healthy and diseased fish and three other known strains of U14, TF7 and ATCC7966 were used for comparison. They were identified using LD50 determination, haemolytic activity, DNA-DNA hybridization and analysis of their …
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The Potential of Coelomocyte Chemotaxis as an Immune Biomarker in the Earthworm, Lumbricus terrestris
… sheep erythrocytes, Saccharomyces cerevisiae, Aeromonas hydrophila, Eisenia fetida and Rhabditis pellio were characterized. Chemotaxis was reported to LPS, FMLP, sheep erythrocytes, S. cerivesae and E. fetida. Bio-indicator potential of chemotaxis is discussed relative to variability in …
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Influence of copper on resistance of Lumbricus terrestris to bacterial challenge
… intracoelomically with two bacterial species, Aeromonas hydrophila and Pseudomonas aeruginosa, and mortality rates were observed. Neither were found to be particularly pathogenic at injected doses of up to 108 bacteria per earthworm. The influence of Cu++ (as CuSO4) on the earthworm's response …
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Structural characterization of chaperone and substrates from the type three secretion system of bacterium aeromonas hydrophilla
Aeromonas hydrophila is a known opportunistic pathogen that infects fishes and humans. This gram-negative pathogen utilizes the Type Three Secretion System to transport effector proteins into the host cell. Several proteins are essential for the transportation of these effectors, they are the …
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Microbiological evaluation of the Mhlathuze River in KwaZulu-Natal
… spp.r Serraiia spp,, Klebsiella spp. and Aeromonas hydrophila. The average monthly pH values ranged between 6.5 and 8.5. The turbidity, dissolved oxygen, hardness, ortho- and total phosphates values obtained did not show any major changes that would call for caution. A polymerase chain …
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Comparative Whole-genome and Phenotype Analyses of Virulence Factors and Lethality in Nematode Challenge Assay among Aeromonas isolates
… and wound-infections in humans and motile <em>Aeromonas</em> septicemia in fish. Although many virulence factors have been described, determining which factors are most important in pathogenesis is ongoing. To examine this further, the genomes of five <em>Aeromonas</em> strains isolated from …
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Studies on the occurrence of antibiotic-resistant bacterial strains from agriculture-associated waters and their proteomic and genomic characterizations
… viz. Enterobacter spp., Burkholderia cepacia, Aeromonas hydrophila, Enterobacter agglomerans, and Pseudomonas stutzeri that were resistant towards ampicillin, cotrimoxazole, novobiocin and tetracycline were successfully identified. Furthermore, the incidence of plasmid mediated and efflux pump …
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Enhancing Seafood Quality and Safety by Reducing Reliance on Antibiotics: Applying a Novel Antibody in Tilapia
… novel antibody diet, for the bacterial pathogen, Aeromonas hydrophila, in tilapia. The tilapia were on an anti-interleukin-10 antibody diet as a neutralization of interleukin-10 (IL-10), an anti-inflammatory cytokine. The anti-inflammatory function of IL-10 has been shown to allow persistence of …
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Effects of Host-derived Probiotics on the Growth Performance and Health of Tilapia and Carp
… <i>in vitro</i> for antagonism against <i>Aeromonas hydrophila</i> and <i>Streptococcus iniae</i>. The top 31 isolates with inhibitory effects underwent screening against three further fish pathogens and were assessed for haemolytic activity and extracellular enzyme production <i>in …
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Characterizing the Role of the Type VI Secretion System in Interbacterial Species Interactions and Pathogenesis
… T6SS strains, Vibrio cholerae V52 and Aeromonas hydrophila SSU, both species co-existed despite active bacterial killing. Fluorescence microscopy analyses revealed survival was possible through the formation of sister-cell clusters. Cluster formation was dependent on T6SS effector …
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Wirkung des Ballastwasser-Biozids Acrolein auf verschiedene pathogene Bakterienstämme in Meer-, Brack- und Süßwasser
… Serratia liquefaciens, Pseudomonas aeruginosa, Aeromonas hydrophila, Vibrio parahaemolyticus, Vibrio alginolyticus und zwei Stämme Vibrio vulnificus. Basierend auf der ScanMIC-Methode (Rahman et al, 2003) wurden Mikrotiterplatten mit Bakterienflüssigkultur, Acrolein und Probenwasser …
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