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Showing 1 to 20 of 50 for “"Aeromonas"”.
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Aeromonas bakteriofago KLEA5 uodegėlės baltymo gp45 tyrimai /
… gp45 protein of the KLEA5 phage that infects the Aeromonas veronii KR2-5 isolate has been studied. KLEA5_gp45_pET21a and KLEA5_gp45_pCDFDuet-1 gene expression vectors were amplified. Soluble ~70 kDa gp45_N-his and gp45_C-his proteins were obtained by gene expression in E. coli BL21(DE-3) cells. …
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Structural characterization of the type II secretion system of Aeromonas hydrophila
… exeC gene, found in the gram-negative bacteria Aeromonas hydrophila codes for a 31 kDa, three domain, bitopic inner membrane protein. The components of the ExeC protein include an amino-terminal cytoplasmic domain, a trans-membrane helix and two periplasmic domains. The two periplasmic domains …
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Investigation of the distribution of motile Aeromonas within Grampian water supplies.
Aeromonas is ubiquitous in freshwater aquatic environments covering a wide range of trophic conditions. Its association with gastrointestinal disease has led various workers to investigate the prevalence and significance of Aeromonas in drinking water supplies. This examination of a Scottish public …
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Chemical, Physical and Catalytic Properties of Tryptophanase Purified From Aeromonas Liquefaciens
Made available in DSpace on 2014-12-11T18:16:52Z (GMT). No. of bitstreams: 1 7317166.pdf: 5131557 bytes, checksum: d234db5ea6f0a4e37621fa4afd1e0886 (MD5) Previous issue date: 1972
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Isolation and Use of the Aeromonas hydrophila Secretin ExeD for Nanopore Analysis
… A larger pore, using the type II secretin of Aeromonas hydrophila, may be able to circumvent the size constraint of α-hemolysin and could allow research into larger molecules to be made. The goal of this project was to isolate and use the type II secretin ExeD in nanopore analysis. After this …
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Investigation of mesophilic Aeromonas: response to hydrogen peroxide and role in false-positive Colilert reaction.
Mesophilic Aeromonas are opportunistic human pathogens which produce a wide range of virulence factors and have been isolated from both untreated and chlorinated drinking waters. The presence of these microorganisms in the distribution systems suggests that Aeromonas could display an adaptive …
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Structural characterization of chaperone and substrates from the type three secretion system of bacterium aeromonas hydrophilla
Aeromonas hydrophila is a known opportunistic pathogen that infects fishes and humans. This gram-negative pathogen utilizes the Type Three Secretion System to transport effector proteins into the host cell. Several proteins are essential for the transportation of these effectors, they are the …
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Isolation, Characterisation and Preservation of Bacteriophages for Use in Treatment of Aeromonas hydrophila Infection In Finfish
… suitability of using lytic phages in controlling Aeromonas hydrophila infection in fish aquaculture. A. hydrophila is a fish pathogen and responsible for economic damages to the aquaculture industry. The main focus of this study was to develop and use a suitable model to test the efficacy of a …
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Comparative Whole-genome and Phenotype Analyses of Virulence Factors and Lethality in Nematode Challenge Assay among Aeromonas isolates
… and wound-infections in humans and motile <em>Aeromonas</em> septicemia in fish. Although many virulence factors have been described, determining which factors are most important in pathogenesis is ongoing. To examine this further, the genomes of five <em>Aeromonas</em> strains isolated from …
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Mechanism of action of AexU, a new type III secretion system effector from an emerging human pathogen Aeromonas hydrophila
Our laboratory first reported the complete sequence of the type III secretion system (T3SS) from a diarrheal isolate SSU of A. hydrophila. We identified an effector protein (designated as AexU) of the T3SS, which exhibited ADP-ribosyltransferase (ADPRT) and GTPase-activating protein (GAP) activity. …
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Delineation of Aeromonas hydrophila Pathotypes by Dectection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay
<p><em>Aeromonas hydrophila </em>is a Gram-negative, bacterial pathogen of humans and other vertebrates. To delineate pathotypes, the genomes of twenty-eight <em>Aeromonas </em>isolates<em> </em>were screened by PCR to determine the presence of virulence factors including: aerolysin …
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Evidence of Water to Human Transmission of Aeromonas Hydrophila: Critical Role of Quorum Sensing in Bacterial Virulence & Host Response
Aeromonas hydrophila is a Gram-negative bacterium which produces a wide variety of virulence factors leading to both intestinal and extra-intestinal infections in humans. A large number of water and clinical Aeromonas isolates were molecularly finger-printed and our data provided the evidence of …
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The effect of temperature upon the growth and metabolism of aeromonas hydrophila and lactobacillus plantarum in pure and mixed culture
… and metabolism of pure and mixed populations of Aeromonas hydrophila and Lactobacillus p/an/Qrum were studied. Initially a medium was developed to provide unbiased support for both organisms. The effect of temperature upon lag phase, growth rate, and final population level between pure and mixed …
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Role of new virulence mechanisms/factors (type 3 secretion system and TOX-R regulated lipoprotein [TAGA]) in the pathogenesis of the emerging human pathogen Aeromonas hydrophila
Aeromonas hydrophila, a gram-negative bacterium that causes gastroenteritis, wound infections, septicemia, and other diseases in humans, produces many different virulence factors. A clinical isolate SSU of A. hydrophila possesses a cytotoxic enterotoxin Act, a potent virulence factor that is …
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Important role of the type VI secretion system in the virulence of an emergent human pathogen Aeromonas hydrophila: Delineating the mechanism of action of its effectors
Aeromonas hydrophila causes various human diseases, including gastroenteritis, wound infections and septicemia. In this study, we characterized the new type 6 secretion system (T6SS) from isolate SSU of A. hydrophila and demonstrated its role in bacterial virulence. We have provided evidence that …
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CHARACTERIZATION AND PATHOLOGICAL STUDY OF A. HYDROPHILA IN WALKING CATFISH (CLARIAS GARIEPINUS)
In this study, 18 strains of Aeromonas hydrophila had been isolated from healthy and diseased fish and three other known strains of U14, TF7 and ATCC7966 were used for comparison. They were identified using LD50 determination, haemolytic activity, DNA-DNA hybridization and analysis of their …
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The Potential of Coelomocyte Chemotaxis as an Immune Biomarker in the Earthworm, Lumbricus terrestris
… sheep erythrocytes, Saccharomyces cerevisiae, Aeromonas hydrophila, Eisenia fetida and Rhabditis pellio were characterized. Chemotaxis was reported to LPS, FMLP, sheep erythrocytes, S. cerivesae and E. fetida. Bio-indicator potential of chemotaxis is discussed relative to variability in …
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Investigation of bacterial populations in a biological nutrient removal system
… major groups of phosphorus storing bacteria, Aeromonas/Vibrio, coliforms, Pseudomonas spp and Acinetobacter spp, were recovered. The identification of cultures on denitrification medium also recovered Pseudomonas, Aeromonas, coliforms and Acinetobacter, indicating the overlap in the function …
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TEMPORAL DYNAMICS, ANTIMICROBIAL RESISTANCE AND PHYLOGENETIC RELATIONSHIPS OF BACTERIAL TAXA IN IRRIGATION WATER SOURCES AND RELEVANCE TO FOOD SAFETY
… and unknown/understudied pathogens such as Aeromonas that are associated with foodborne outbreaks. To facilitate the adoption of microbiologically safe irrigation water sources, a comprehensive study on the prevalence and virulence potential of human pathogens and their transferability to …
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Influence of copper on resistance of Lumbricus terrestris to bacterial challenge
… intracoelomically with two bacterial species, Aeromonas hydrophila and Pseudomonas aeruginosa, and mortality rates were observed. Neither were found to be particularly pathogenic at injected doses of up to 108 bacteria per earthworm. The influence of Cu++ (as CuSO4) on the earthworm's response …
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