Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"AdoMet"”.
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EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT
… ≥ 99% depletion of plasma S-adenosylmethionine (AdoMet) levels in both "Pneumocystis" pneumonia (PcP) animal models and patients. AdoMet is a critical cellular metabolic intermediate, with a pivotal role as methyl donor in a myriad of biochemical processes and necessary for the synthesis of the …
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Structural studies of S-adenosyl-L-methionine radical enzymes involved in tRNA and natural product biosynthesis
Members of the S-adenosyl-L-methionine (AdoMet) radical enzyme superfamily catalyze a myriad of diverse and challenging biotransformations using a [4Fe-4S] cluster and a molecule of AdoMet to initiate radical. In this thesis, we used a combination of crystallographic and biochemical methods to …
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The Role of S-Adenosylmethionine Synthetase in Killing Effects of DFMO in African Trypanosomes
… work was to investigate the role of a functional AdoMetSyn in the toxic effect of eflornithine, a clinically used therapy for the treatment of the disease. Prior studies suggested that the toxicity of eflornithine may be due to the combined effect of polyamine depletion and detrimental effects of …
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Neue Anwendungen von (S)-Adenosyl-L-methionin-Analoga mit Protein-Methyltransferasen und Click-Chemie zur sequenzspezifischen Markierung von Proteinen
… The ubiquitous cofactor S-adenosyl-L-methionin (AdoMet) is the major methyl donor for enzymatic methylation reactions. Protein methyltransferases (MTases) catalyse methyl group transfer to target amino acid residues. However, the methyl group is a poor chemical reporter. To transfer larger …
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The roles of redox active cofactors in catalysis : structural studies of iron sulfur cluster and flavin dependent enzymes
… characterization of the S-adenosyl-L-methionine (AdoMet) radical superfamily showed the versatility of these cofactors. In this superfamily, which includes over 40,000 unique sequences, [4Fe-4S] clusters are responsible for the initiation of radical chemistry. A recently described subclass of this …
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Strukturanalyse der Methyltransferasen AviRa und AviRb aus S. viridochromogenes
… des Kofaktors S-Adenosyl-L-methionin (AdoMet) auf jeweils eine spezifische Base im Peptidyltransferase-loop der 23S-rRNA und verhindern so die Bindung des endogenen Antibiotikums Avilamycin in diesem Bereich. <br>Beide Enzyme wurden in E. coli exprimiert. Zur Reinigung von AviRa diente …
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Viengrandines mažąsias RNR modifikuojančių gyvūnų Hen1 metiltransferazių taikymas RNR žymėjimui ir sekoskaitai /
… and catalyze the S-adenosyl-L-methionine (AdoMet) dependent methylation of the 2'-O-ribose of the 3'-terminal nucleotide in a subset of small non-coding RNAs. This modification protects small RNAs from degradation, meanwhile the deficiency of the Hen1 protein causes detrimental phenotypic …
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Biochemical and Structures Studies of tRNA Modificaton and Repair Enzymes
… 3' ends at the 2' OH position by Hen1, an S-Adomet dependent methyltransferase. Hen1 homologs in animals are shown to be responsible for the methylation of piRNAs, some siRNAs as well as endogenous siRNAs. Interestingly, a subset of bacteria also has a Hen1 homolog. Since bacteria do not have …
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A directed evolution design of target specificity and kinetic analysis of conformational transitions in the HhaI methyltransferase /
… transfer of the methyl group from the cofactor AdoMet to the C5-position of a target cytosine. In this work both these aspects of the MTase mechanism have been addressed. First, using rational protein design and directed evolution approaches the specificity of the HhaI MTase, GCGC, has been …
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Studies of thiamine biosynthesis
… with the ‘radical S-adenosylmethionine’ (AdoMet) family of proteins, including conserved cysteine ligands to the essential [4Fe-4S] cluster and has been shown to form a complex with ThiG. With the purpose of studying the mechanistic enzymology by which Thz-P is assembled it was crucial to …
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Aufklärung der Funktion von zwei rRNA-Methyltransferasen und Untersuchungen zur Eurekanat-Biosynthese in Streptomyces viridochromogenes Tü57
… seine eigenen Ribosomen durch zwei AdoMet-abhängige Methyltransferasen schützt (AviRa und AviRb), die spezifische Nukleotide der 23S rRNA modifizieren. Verkürzte in-vitro-Transkripte der Domäne V der 23S rRNA dienten als Substrate für Methylierungsversuche mit beiden heterolog …
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Cloning, Heterologous Expression, and Characterization of Human and Escherichia Coli Choline Dehydrogenases
… of one-carbon units for cytosolic folate- and AdoMet-dependent reactions, while in E. coli, betaine-glycine, the final product of choline oxidation, acts as an osmopretectant compatible solute that accumulates when the cells are exposed to drastic environmental changes in osmolarity. …
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A TALE OF TWO METHYLATION MODIFICATIONS IN ARCHAEAL RNAs
… was reconstituted. The methylation reaction is AdoMet-dependent. The efficiency of the methylase reaction depended on the identity of the residue at position 55 of the TØ-loop. The presence of Ø55 allowed the efficient conversion of Ø54 to m1Ø54, whereas in the presence of C55 the reaction was …
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In Vitro Studies on the Methylation of DNA in Rat Brain and Liver Chromatin
… methyltransferase for S-adenosyl-L-methionine (AdoMet) was 3.5 ± 0.15 μm and 4.2 ± 0.13 μm, in the liver and brain, respectively. The Vmax values for the enzyme from liver and brain of 5-day-old rats were 0.17 ± 0.04 and 0.23 ± 0.09 pmol of <sup>3</sup>H-methyl groups incorporated/mg …
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Funktionsbasierte Isolierung von Subproteomen mit trifunktionellen Fangverbindungen
Isolation, identification and quantitation of functional subproteomes by use of trifunctional capture compounds™, followed by mass spectrometric analysis, is a relatively new, promising technology for proteomic research. Particularly for analysis of effects and side effects of drugs or plant …