Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Adenylate Kinase"”.
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Regulation of Adenylate Kinase
… of the mutant enzyme. This protein, called an adenylate kinase-associated protein, co-purified with the wild type and mutant enzymes through several purification steps. A homogeneous preparation of the adenylate kinase-associated protein gave a single band on a sodium dodecyl …
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Protein Dynamics: Studies of Adenylate Kinase Mutants From Escherichia Coli and Characterization of Adenylate Kinase Isoforms From Murine Cells. Applications of Fluorescence Spectroscopy and Microscopy
… thesis is focused on the characterization of two adenylate kinase isoforms (AK1 & AK1beta) from murine cells using a series of fluorescence microscopic techniques (two-photon excitation fluorescence imaging, fluorescence correlation spectroscopy (FCS), fluorescence lifetime imaging (FLIM)). In the …
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Gene cloning, adjustable expression and site-directed mutagenesis of Escherichia coli adenylate kinase
The gene encoding the Escherichia coli adenylate kinase has been cloned by phage Mu-mediated transposition. Subcloning of the adk$\sp{+}$ gene into pUC13 and pBR322 allowed high-level expression and a simple purification scheme for the enzyme. An optimized system was developed for the in vivo …
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Investigations of Local Unfolding Like Conformational Excursions in the Native State of Adenylate Kinase
… the binding interaction of Escherichia coli adenylate kinase (AK). We rationally design several single-site, surface-exposed glycine mutations to selectively perturb the excited state conformational repertoire, leaving the ground-state X-ray crystallographic structure unaffected. The results …
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Population Frequencies of Phosphoglucomutase (PGM), Adenylate Kinase (AK), and Haptoglobin (Hp) in the Metropolitan Birmingham Area.
Frequently the need to identify or exclude an individual arises in criminal cases. Historically, fingerprinting has been a universally acceptable method of individual identification. Unfortunately, fingerprints are not always found at the crime scene ; therefore, other methods for identification of …
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Spectroscopic Studies of Protein Folding and Protein Dynamics Using Single-Tryptophan Mutants of E. Coli Adenylate Kinase
… of the various equilibrium states of E. coli adenylate kinase is presented in the final summary chapter."
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Fluorescence characterization of protein and lipid dynamics
… used to study the motion of protein domains in adenylate kinase, and to characterize the formation of myelin in neuron Schwann cell co-cultures. Domain motion upon substrate binding was examined in adenylate kinase using fluorescence resonance energy transfer. The process of myelin formation was …
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Contributions to the analysis of proteins
… in a conformational change pathway analysis of adenylate kinase. Next, we present a new algorithm to account for the effects of solvent-damping on slow protein conformational dynamics. The algorithm proves to be an effective approach to calculating the diffusion coefficients of proteins with …
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Identification and characterisation of novel marker proteins involved in X-linked muscular dystrophy
… proteins involved in energy metabolism including adenylate kinase and many ATP synthase isoforms. Reduced concentrations were also observed in numerous contractile proteins and intermediate filament proteins. With the loss of these structural elements, a drastic increase in stress proteins was …
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Computational Modelling of Nanoscale Interactions for Nanophotonics and Biosensing
… biosensor signals from the catalytic cycle of Adenylate Kinase (AdK); a large protein with more than 3000 atoms. We assume that the AdK molecule is situated in a region of high field intensity (plasmonic hot spot) to ensure that the molecule can be detected above the noise threshold of the WGM …
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The proteomic analysis of B-cell receptor signaling in chronic lymphocytic leukaemia
… with the activation of anaplastic lymphoma kinase (ALK), the plasma kallikrein-kinin system (KKS), the AKT-1 pathway, the MAPK pathways, the adenylate kinase system and involvement in the CD40-dependant activation of B-CLL cells. One of the protein targets found (kininogen) was increased by …
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Tumour chemo sensitivity assays: an investigation into the susceptibility of cells to chemotherapeutics
… measures the release of the intracellular enzyme adenylate kinase into the culture supernatant after membrane rupture and is evaluated as an indicator of cell death. This assay was proven to correlate and compete with currently used methodologies for the assessment of cytotoxicity and with its …
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Charakterisierung von Adenylatkinasen aus Plasmodium falciparum und Thioredoxinreduktase-assoziierten Proteinen aus Dipteren
… benötigen sie hohe Aktivitäten an Adenylatkinase (AK; ATP + AMP 2 ADP) und GTP-AMP-Phosphotransferase (GAK; GTP + AMP GDP + ADP). Beide Enzyme wurden in Blutstadien des Malariaparasiten Plasmodium falciparum identifiziert und die entsprechenden Gene der PfAK und PfGAK auf den …
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An in vitro and in vivo hepatotoxicity study of an aspalathin- rich green rooibos extract
… and membrane integrity was assessed using the adenylate kinase enzyme (AK). For the in vivo study, sub-chronic Sprague Dawley rats were used to investigate the toxicity of different concentrations of GRT extract. The rats were randomly divided into five groups with ten rats per group. Three of …
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Risk Factors, Mechanisms and Therapeuthic for Right Heart Failure Associated with Pulmonary Hypertension
Right ventricular function (RV) is one of the most important predictors of prognosis in many cardiovascular disease states. Despite the significance of RV function to survival, there are no therapies that directly nor selectively improve RV function. As well, the basis for RV failure is poorly …