Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Adenoviral Vectors"”.
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THE USE OF REPLICATION-DEFECTIVE ADENOVIRAL VECTORS FOR EXPRESSION OF PRIMORDIAL ATHEROGENIC LIPOPROTEINS IN MICE
The plasma concentration of apolipoprotein B (apoB)-containing lipoproteins is positively associated with several diseases, including atherosclerosis, type 2 diabetes and obesity. Hence, apoB may be a prime target for therapeutic intervention, particularly during its assembly. The molecular events …
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Cytokine gene transfer by adenoviral vectors as a novel therapeutic option for hepatitis B virus infection
… of interferon on HBV replication. Two sets of adenoviral vectors were established: the first using the CMV promoter (PCMV) (AdmIFNg and AdmIFNb) and the second using a bidirectional tetracycline (tet)-regulated promoter(Pbi-1) (Adbiluc vectors) to locally express mouse interferon (mIFN) a, b …
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Targeted delivery of biological agents
… peptides and gene delivery systems (recombinant adenoviral vectors), suffer from a relative lack of native targeting capacity. Improvement of their targeting capacity could significantly increase the efficiency of these agents to provide therapeutic effects. Selective targeting of an anti-oxidant …
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Studies of human T cell costimulation: potential for the immunotherapy of cancer
… for cancer immunotherapy. Using recombinant adenoviral vectors encoding the costimulatory molecules CD80 and 4-1BBL (CD137L) and the cytokine IL-12 tumour cell lines were transduced to express these molecules individually or in combination in vitro. Using PBMC from healthy donors the effect …
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Ex vivo culture of patient tissue and examination of gene delivery
… targeting. The greatest challenge gene delivery vectors face is the ability to safely and efficiently deliver genes into target cells. The overall objectives of this thesis are to evaluate the efficacy of various gene delivery methods in a clinically relevant tumour model and to also investigate …
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Use of a direct, positive selection strategy to generate improved prodrug-activating enzymes for cancer gene therapy
… mutant to date behind T41L N71S (0.90 µM s-1). Adenoviral vectors were generated and their ability to sensitise SKOV3 cancer cells tested. T41G N71S was ~2-fold improved relative to WT NTR. Purified enzymes were also tested with SKOV3 cells, with T41G N71S being ~2.6-fold improved relative to WT …
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TIS21 shifts p53-induced response of EJ bladder carcinoma cells from senescence to apoptosis
… and induces cellular senescence. Employing adenoviral vectors carrying p53 (Ad-p53) or TIS21 (Ad-TIS21) gene, I evaluated the effect of TIS21 on the p53-induced senescence phenotypes in EJ cells. Adenoviral transfer with wild-type p53 significantly induced senescent phenotypes of EJ cells …
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Metabolic biotinylation of the adenoviral capsid: Avidin-based applications and studies of ligand-targeted gene delivery
Adenoviral vectors have great potential for use in gene therapy and genetic immunization. The targeting of Ad vectors to the relevant tissue and cell types in vivo could greatly improve their safety and performance by lowering the effective dosage required for therapeutic levels of gene expression. …
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OAdV7, an ovine adenoviral vector as a novel vaccine vector for tumour immunotherapy
… making them one of the most popular viral vectors for vaccine development. To date, the majority of the studies in the literature have focused upon human adenovirus serotype 5 (HAdV5) for the development of adenoviral vectors. However, the use of HAdV5 is limited due to its high prevalence …
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Visualization and quantification of autophagy in primary gastrointestinal epithelia cell cultures.
… study human colon crypts cultures transduced by adenoviral vectors expressing GFP-LC3. In comparison to cell lines after starvation, there were less GFP LC3 puncta in the smaller epithelial cells from colon crypt tissue (20-30 compared to 5.8+/-2.2). MDP generated significantly more LC3 puncta …
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Ebna1-Specific T Cell Responses During Persistent Rhesus Lcv infection and The Development of a Novel Therapeutic Prototype Vaccine for Ebv-Associated Diseases
… two serologically distinct replication-defective adenoviral vectors that expressed chimeric rhEBNA1 constructs fused to functional and non-functional versions of Herpes Simplex Virus- glycoprotein D (HSV-gD). HSV-gD has been shown to augment T cell responses by inhibiting the immunosuppressive …
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Construction of bovine adenovirus type 3 E1 and E3, substitution plasmids and the sequencing analysis of DNA pol and pTP /
… a suitable candidate for the construction of vectors. The use of adenoviral vectors in gene therapy, vaccination, and as a general vector system for expressing foreign genes have been documented for some time. In this study, the objective was to rescue a BAV3 E1 or E3 recombinant vector …
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Investigating viral subversion of intercellular communication
… that gap junctions would be targeted during adenoviral infection. We find reduced Cx43 protein due to suppression of GJA1 transcription dependent upon β-catenin during adenoviral infection, with viral protein E4orf1 sufficient to induce β-catenin phosphorylation. Loss of gap junction function …
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Base editing of Galactose-1-Phosphate Uridylyl Transferase as a novel gene therapy approach to treat Q188R mutation in a cellular model of Classic Galactosemia
Classic Galactosemia (CG) is a rare genetic disorder represented by the inability to convert galactose to glucose. A mutation at the galactose-1-phosphate uridylyltransferase (GALT) enzyme coding gene halts galactose metabolism which leads to the accumulation of galactose-1-phosphate and …
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Therapeutic Gene Delivery to Human Pancreatic Islets for Treatment of Diabetes and the Effect of TFO on Liver Fibrosis Induced by Bile Duct Ligation
… better known islet transduction efficiency of adenoviral (Adv) vectors, in this study, we constructed Adv‑hVEGF‑hIL‑1Ra by cloning hVEGF and hIL‑1Ra coding sequences and polyA signal under separate cytomegalovirus (CMV) promoters in Adenoquick plasmid (Ad 13.1). There was dose and time …
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Immunologisches Monitoring der Gentherapie des Fibrosarkoms der Katze
… to vector and transgene In the context of an adenoviral gene therapy of the fibrosarcoma of the cat we introduced the human interleukin 2 (huIL 2)-gene and the feline Interferon g (feIFN g)-gene, one or the other or a combination of both. The aim of these studies is to evaluate the toxic risk …