Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Adenoviral Vector"”.
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OAdV7, an ovine adenoviral vector as a novel vaccine vector for tumour immunotherapy
… making them one of the most popular viral vectors for vaccine development. To date, the majority of the studies in the literature have focused upon human adenovirus serotype 5 (HAdV5) for the development of adenoviral vectors. However, the use of HAdV5 is limited due to its high prevalence …
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Quantitative analysis of adenoviral vector modification of a cytokine-mediated cell death decision
… viruses are used extensively as delivery vectors in clinical gene therapy and in molecular biology, but little is known about how the viral carrier itself contributes to cellular responses. In this thesis, we explored the link between viral vector modifications of signaling networks to …
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Entwicklung und Anwendung eines Expressionsklonierungssystems in primären Kardiomyozyten - Identifizierung von Translin als neuem Target der Herzinsuffizienz
… heart library was integrated in an inducible adenoviral vector system. After transduction of cardiomyocytes and induction of the transgenes, cells were analyzed for morphological changes reflecting the hypertrophic process. A new system based on laser scanning cytometry was developed as read …
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Targeting of adenovirus serotype 5 infectivity to EGFRvIII-expressing cells using a bispecific CAR-scFv.
… to investigate a possible means of developing an adenoviral vector (based on subclass C viruses) that can utilize MR1's targeting ability towards EGFRvIII to infect only cancer cells that express this receptor. Adenovirus infectivity is primarily mediated by binding of the globular carboxyl …
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Using machine learning to increase the predictive value of humanized mouse models for the human immune response to YFV-17D
… neighbors (KNN), random forest (RF), support vector machine (SVM), and neural network (NN)). Model predictions were evaluated for accuracy using F-scores and Matthews correlation coefficients. Several algorithms combined with mouse models made significantly better predictions about …
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Interleukin-1 receptor antagonist delivery through adenoviral mediated gene transfer as a treatment for equine joint disease
… published gene sequence for equine IL-1Ra, an adenoviral vector (Ad-EqIL-1Ra) was constructed that was capable of equine IL-1Ra transgene expression. This vector was tested in vitro to ensure its ability to both transduce equine synoviocytes without cytotoxic effects and produce a biologically …
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Human Chimeric Antigen Receptor Macrophages For Cancer Immunotherapy
… macrophages, which are resistant to most viral vectors, are efficiently transduced by the chimeric-fiber adenoviral vector Ad5f35. Ad5f35 transduced primary human CAR macrophages demonstrated targeted phagocytosis, with phagocytic activity dependent on both the CAR and antigen densities. CAR, …
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Quantitative analysis of cytokine-induced hepatocellular death in the context of hepatotoxic therapeutics
Numerous therapeutics, such as viral gene therapy vectors, have unintended toxicity in part due to interactions with inflammatory cytokine signaling to elicit hepatocyte death, thus limiting their clinical use. Although much is known about how cytokines and certain therapeutics individually induce …
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Cytokine gene transfer by adenoviral vectors as a novel therapeutic option for hepatitis B virus infection
… of interferon on HBV replication. Two sets of adenoviral vectors were established: the first using the CMV promoter (PCMV) (AdmIFNg and AdmIFNb) and the second using a bidirectional tetracycline (tet)-regulated promoter(Pbi-1) (Adbiluc vectors) to locally express mouse interferon (mIFN) a, b …
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A Role for Mindbomb 1 in Adenovirus Genome Delivery
… design of new antiviral treatments and emerging adenoviral vector-based therapies.</p>
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Making In Utero Gene Therapy Safer and More Efficient: A First Step Towards Clinical Realization
… to improve the efficacy and safety of viral vectors. A two-vector delivery system was constructed to perform cell-specific in utero gene therapy, and I showed proof-of-principle that the system functions in vitro in human and sheep cells. An Ad5 serotype adenoviral vector (Ad5) acts as the …
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The New Role of Proprotein Convertase Subtilisin/Kexin Type 9: A Connection of Proprotein Convertase Subtilisin/Kexin Type 9, Apolipoprotein B, and Autophagy
… cholesterol levels. We used a second-generation adenoviral vector to overexpress PCSK9 (Ad-PCSK9) in wild-type C57BL/6 and LDLR deficient mice (<em>Ldlr-/-</em> and <em>Ldlr-/-Apobec1-/-).</em> Our study revealed that overexpression of PCSK9 promoted the production and secretion of apoB in the …
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Investigating the functions of PGC-1 isoforms in retinal pigment epithelia metabolism and their implications on age-related macular degeneration
… of our novel tetracycline-inducible PGC-1β adenoviral vector showed that upregulation of PGC-1β was efficiently controlled by the addition of doxycycline to transfected cells. Upon exposure to H2O2, transfected cells treated with doxycycline experienced greater cell death than transfected …
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Regulation and patterning of cell differentiation and pluripotency
… modulator of inducible BMP-2 expression vector, to mesenchymal stem cells cultured in a microfluidic 3. Patterning of the Nanog gene expression in embryonic stem cells, using a microfluidic device, to establish differentiation - pluripotency boundaries that mimic the developmental …
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Identification of a conserved alternative mRNA splicing program that supports hepatic growth and maturation during development and regeneration
… in liver cell lines HepG2 and AML12, using an adenoviral vector. These cell lines have minimal expression of ESRP2 and exhibit a neonatal splicing pattern. Forced expression of ESRP2 causes a fetal-to-adult splicing switch in the majority of these 31 targets, reinforcing the role of ESRP2. When …