Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Adenosine Diphosphate"”.
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Production of poly(adenosine diphosphate-ribose) polymerase-1: development of an efficient production and purification protocol
PARP-1 is a nuclear enzyme involved in a range of activities associated with DNA metabolism, playing a key role in maintaining the integrity of DNA and chromatin structure. As such, this enzyme is likely to provide a useful target when using a rationale drug design approach to develop …
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Investigating the Role of PARylation in Regulating Skeletal Muscle Mass and Function in Healthy Mature Mice
Adenosine diphosphate (ADP) ribosylation is a post-translational modification dependent on the transfer of ADPr units from nicotinamide adenine dinucleotide (NAD+) on to a plethora of biomolecules (i.e., proteins, DNA, RNA, etc.) in response to physiological stressors (i.e., nutrient deprivation, …
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The Effects of 3-Phosphoglycerate and Other Metabolites on the Activation of AMP-Activated Protein Kinase by LKB1/STRAD/MO25
… (G1P), glucose-1,6-bisphosphate (G1,6-P2), adenosine diphosphate (ADP), carnitine (Carn), acetyl-carnitine (Acarn), inosine monophosphate (IMP), inosine, and ammonia for allosteric regulation. We found that 3PG stimulated LKB1-STRAD-MO25 activity and allowed for increased AMPK …
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Canine Platelet Concentrates: An In Vitro Study to Effectively Provide a Source of Functional Platelets
… by aggregation studies using collagen and adenosine diphosphate as agonists. Product sterility was maintained. There was no consensus of data supporting superior performance of one platelet storage container. This study indicates that canine platelet concentrates may be harvested by …
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Exploring Zirconia as a Column Packing Material
… and analysis of phosphorylated proteins. Adenosine triphosphate, Adenosine diphosphate and Adenosine monophosphate were used as prototypes for phosphorylated proteins. Separation, absorption, fluorescence and SEM studies were performed to determine the adsorption of Adenosine phosphates …
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Spectrum and Incidence of Primary and Therapy-Related Hematologic Malignancies In Individuals With Brca1 and Brca2 Pathogenic Variants
… such as radiation, chemotherapy, and poly (adenosine diphosphate-ribose)-ADP polymerase (PARP) inhibitors. Preliminary evidence suggests that germline <em>BRCA1</em> and <em>BRCA2</em> pathogenic and likely pathogenic (P/LP) variants may increase susceptibility to t-MNs due to the genes’ …
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The Molecular Mechanism of Platelet Activation in Inflammation
… multiple receptor pathways, possibly including adenosine diphosphate (ADP), thromboxane A2 and toll-like receptors (TLRs), to drive platelet activation. This highlights the potential role of extracellular histones in thrombo-inflammatory diseases, including sepsis, trauma, and autoimmune …
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DEVELOPING SMALL-MOLECULE PROBES AND INHIBITORS FOR ADP-RIBOSE-BINDING PROTEINS
… post-translational modifications (PTMs), protein adenosine diphosphate ribosylation (ADP-ribosylation) serves as key effectors and regulators in a plethora of biological processes. Although the biological roles of mono-ADP-ribosylation (MARylation) and poly-ADP-ribosylation (PARylation) have been …
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USING CHEMICAL AND PROTEOMICS APPROACHES TO STUDY THE FUNCTIONS OF SIRT6 AND KRAS
… deacetylation, lysine defatty-acylation and mono-adenosine diphosphate (ADP)-ribosyltransferase activities. However, the contribution of each activity to the various functions of SIRT6 is poorly understood. In Chapter 2, I describe the dissection of functional contribution of the defatty-acylase …
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Anti-platelet aggregation activity of melaleuca bracteata var.revolution gold derived betulinic acid and its derivatives
… against four agonists (thrombin, collagen, adenosine diphosphate and epinephrine) used separately to induce platelet aggregation. The ability of the compounds to separately inhibit the hydrolysis of chromogenic substrate was used for antithrombin activity of the triterpenoids. The release of …
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Adiponectin : a novel circulating anti-thrombotic factor in humans?
… that gAd-stimulated aggregation required adenosine diphosphate (ADP) and thromboxane A2 (TxA2) to achieve the maximal response. Consistent with this, threshold concentrations of gAd could synergise with ADP. However, using receptor antagonists this synergy was found to occur via the …
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Distinct functional effects of NMDA receptors in normal and leukaemic megakaryocytes
… in Meg-01 cells and amplifying Ca2+ responses to adenosine diphosphate (a known regulator of megakaryocytic maturation). We found that NMDAR agonists increased and NMDAR antagonists reduced growth and proliferation of Meg-01, Set-2 and K-562 cells. Unexpectedly, NMDAR antagonists also facilitated …
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The Effect of AICAR Treatment on Sarcoplasmic Reticulum Function and Possible Links to Skeletal Muscle Fatigue
… and slowed relaxation. During exercise, adenosine 5'-triphosphate (ATP) is the energy currency that is used to support force production. As a result of ATP hydrolysis and re-synthesis, adenosine diphosphate (ADP) and adenosine monophosphate (AMP) levels rise. AMP kinase (AMPK) is an …
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Development and Application of Chemoenzymatic Tools to Investigate Site-Specific Protein ADP-Ribosylation
Herein I have developed the first technologies to install mono- and poly-ADP-ribosylation at specific sites on synthetic peptides and full-length proteins. Historically, chemical and topological complexities associated with ADP-ribosylation have stymied progress in this area. To overcome this, I …
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Magnesium Transport In Mammalian Erythrocytes And Its Effect On Platelet Aggregation
… employing different concentrations of adenosine diphosphate (ADP) on the plasma from both pre- and post-dialysis patients. Healthy human controls were used as a comparison. The Mg 2+ level in plasma from both Pre- and post-dialysis patients was slightly lower than in human control. In …
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Investigating small-molecule inhibitors of platelet aggregation
… thrombosis, driven by platelet aggregation. Adenosine diphosphate (ADP)-induced platelet aggregation is mediated by the Gi-protein-coupled receptor (GPCR), P2Y12. Therefore, P2Y12 antagonists are clinically used to prevent thrombotic events. However, current antiplatelet drugs have several …
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An investigation of the role of ACTN1 in platelet formation and of platelet function in myeloma
… hyporesponsiveness of platelets to agonist [adenosine diphosphate (ADP), thrombin receptor-activating peptide 6] stimulation in-vitro was observed, with significantly reduced surface levels of P-selectin in response to ADP in patients with MGUS. Platelet-associated immunoglobulins were …
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Isolation and characterization of starch, starch biosynthetic genes and protease inhibitors from Marama beab (Tylosema Esculentum)
… (PCR) based strategy was used to isolate Adenosine diphosphate-glucose pyrophosphorylase (AGPase) and Starch Branching Enzymes (SBEs) using degenerative primers designed at the conserved motif of corresponding cloned plant starch synthesizing genes. Detection of serine protease inhibitor …
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Chemical Genetic and Genomic Discovery of PARP-1-Dependent Mechansims of Transcriptional Regulation
Poly(ADP-ribose) polymerases, or PARPs, are a family of enzymes that modulate diverse biological processes through covalent transfer of ADP-ribose from NAD+ onto target proteins. These targets of post-translational modification as well as the genomic targets, or binding sites, to which the nuclear …
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