Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 76 for “"Adeno-associated Virus"”.
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The Immunology of Adeno-Associated Virus Serotype 2
Adeno-associated virus serotype 2 (AAV-2) has been developed as a gene therapy vector. Antibody and cell-mediated immune responses to AAV-2 or AAV-2 transfected cells may confound the therapeutic use of such vectors in the clinic. In this study, cell-mediated and humoral immune responses to AAV-2 …
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Adeno-associated virus rep78 expression in Arabidopsis thaliana
Adeno-associated virus type 2 (AAV-2) integrates preferentially into a defined site on human chromosome 19, and has been developed as a gene therapy vector. We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate …
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Adeno-Associated Virus-Based Gene Delivery Approaches for Tissue Engineering
… with minimal interference and at low cost. Adeno-associated virus (AAV) is employed as the candidate gene delivery vehicle due to its ability to infect many cell types and good safety profile in humans, which bode well for clinical applications. The body of work is grouped into three parts. …
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Analysis of Cellular Factors Involved in Adeno-Associated Virus Type 2 Entry
<p>The study of virus-host interactions has not only produced insights relevant to both preventative drug design and the emerging field of gene therapy, but it has also served as a valuable tool to the field of cell biology. The biological roles of many pathogen recognized cell-surface receptors …
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Highly efficient Cas9 mediated transcriptional programming and delivery via Adeno-Associated Virus
… only approved vector for human delivery is the Adeno-Associated Virus (AAV). This convenient virus allows for targeting of various tissue types with high efficiency and little risk of integration or immunogenicity. Unfortunately, the virus is limited to a genomic payload of 4.7kb, which can be …
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Mechanism of growth inhibition of primary human cells by adeno-associated virus type 2
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Engineered Replicases, Synthetic DNA, and Long-Read Sequencing Quality Control for Adeno-Associated Virus Production
… can be treated with modified (recombinant) viruses that deliver therapeutic transgenes. Recombinant adeno-associated viruses (rAAV) are the most promising candidates for treatment, as clinical trials have demonstrated the safety of the vector at low and medium-dose, and three AAV-based …
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Investigating intrathecal adeno-associated virus vectors for the study of analgesic interactions at the spinal level
… at the spinal level: develop the use of adeno-associated virus (AAV) vectors for gene-transfer to sensory neurons where the expression of involved proteins can be modulated, and to further characterize these interactions by understanding of the cellular processes underlying their …
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Adeno-associated virus type 2 as vector for human gene therapy: Characterization of virus-host interactions
Vectors based on adeno-associated virus type 2 (AAV) offer considerable promise for somatic gene therapy of various diseases (e.g. cystic fibrosis, hemophilia B, cancer). Limitations, however, still exist and require further improvement. The study presented here addresses two major problems that …
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The role of cellular growth factor receptors in mediating gene transfer by adeno-associated virus 2 vectors
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Cryo-EM reveals how adeno-associated virus engages its receptor AAVR and an investigation of the GPR108 ectodomain
Adeno-associated virus is one of the leading delivery agents used in gene therapy today. While it is already used in the clinic, some aspects of its basic biology are still not yet fully understood. Only recently in 2017 was the primary receptor for most AAV serotypes discovered and there are still …
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Towards the establishment of a highly effective adeno-associated virus production by leveraging next generation sequencing and transcriptomics in HEK293 cells
… the available delivery methods, recombinant adeno-associated viruses (rAAV) have emerged as one of the most promising vectors due to their low immunogenicity, long-term expression even in differentiated cells, and very low rate of integration into the host genome. However, process yields …
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Phenotypic characterisation of the tremor mutant and AAV mediated aspartoacylase gene transfer in the rat model of Canavan disease
… approach to treat Canavan disease, involving Adeno-associated virus mediated delivery of aspartoacylase to the mammalian central nervous system. Gene transfer was undertaken in tremor rat mutants, and analysis was made of gene expression and function as well as the effect of aspartoacylase …
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Adeno-Associated Mediated Transduction of Sensory Epithelia Within the Guinea Pig Cochlea
… R. L., M.S., Autumn 2006 Pharmaceutical Sciences Adeno-associated virus mediated transduction of sensory epithelia within the guinea pig cochlea Chairperson: Dr. David J. Poulsen, PhD. Hearing loss is the most common disability of the senses and affects 250 million people worldwide. This condition …
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Adeno-Associated Mediated Transduction of Sensory Epithelia Within the Guinea Pig Cochlea
… R. L., M.S., Autumn 2006 Pharmaceutical Sciences Adeno-associated virus mediated transduction of sensory epithelia within the guinea pig cochlea Chairperson: Dr. David J. Poulsen, PhD. Hearing loss is the most common disability of the senses and affects 250 million people worldwide. This condition …
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Different Genetic Ways for Conditional Gene Regulation in the Mouse Brain
… as extra-chromosomal elements with recombinant Adeno-Associated Virus (rAAV). First, the pool of tTA expressing mouse lines was increased by generating eight functional itTA-expressing TgThy-1.2(xx)-itTAnls transgenic mouse lines. Since for tTA expression the Thy-1.2 promoter was used, which is …
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Development and application of control tools for use in optogenetics research
… When an actuators packaged into a lox-containing Adeno-associated virus is used in conjunction with a mouse that expresses the Cre recombinase enzyme in a specific cell type, cell specific expression of the opsin is achieved. When used with LEDs, lasers, or specifically fabricated light delivery …
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Using a Mammalian Virus to Create Plants for Site-Specific Transgene Insertion
… insertion of transgenes using features of the adeno-associated virus (AAV) life cycle. In the absence of helper virus, AAV integrates into a specific location on human chromosome 19, the AAVS1 locus. The sequence for AAV integration was introduced into the model plant Arabidopsis thaliana using …
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AAV9-mediated (Pro)renin Receptor Antagonism Reduces High Fat Diet Induced Hyperglycemia and Hepatic Steatosis in Mouse Model of Non-alcoholic Fatty Liver Disease
… causes of liver diseases worldwide. It has been associated with impaired metabolism and increased accumulation of triglyceride (TG) in the liver, most of which is derived from exogenous sources including diet. Recent studies showed involvement of (pro)renin receptor (PRR) in various pathways of …
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