Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Acyltransferases"”.
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DHHC PROTEIN ACYLTRANSFERASES CATALYZES THE LYSINE PALMITOYLATION OF MEMBERS OF THE RAS SUPERFAMILY OF SMALL GTPASES
Protein fatty acylation is the attachment of a long-chain fatty acyl group, generally 14–16 carbons in length to the side chain of an amino acid. Cysteine palmitoylation or S-palmitoylation is best understood and widespread post-translational modification in this class, followed closely by …
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Side chain decoration of Anthocyanins; Mechanisms and Effects On functionality
… of the structural features of anthocyanin acyltransferases that determine their substrate preference. Anthocyanin acyltransferases belong to the BAHD family of enzymes and are involved in acylation. Through mutagenesis I showed that an arginine positioned close to motif-1 in aliphatic …
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Investigating mechanisms of triacylglycerol synthesis induced by nitrogen depletion in the diatom Phaeodactylum tricornutum and characterising its diacylglycerol acyltransferases and phospholipid: diacylglycerol acyl transferase
The ability of the diatom Phaeodactylum tricornutum (P. tricornutum) to accumulate triacylglycerol (TAG) and synthesise the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic (DHA) has led to interest in their development for biofuels and human nutrition. Nitrogen depletion is a …
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Structural Basis for Protein Recognition, Acyl-substrate Delivery, and Product Release by ACP in the Biosynthesis of Lipid A
… to its 4'-phosphopantetheine arm among four acyltransferases: LpxA, LpxD, LpxL, and LpxM. LpxA and LpxD are essential cytoplasmic enzymes, which not only provide an excellent model system to study ACP-based interaction, but also offer an important therapeutic target for development of novel …
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Identification and Biochemical Characterization of Ghrelin O-Acyltransferase (GOAT)
… to a family of 16 polytopic membrane-bound O-acyltransferases. GOAT activity requires catalytic Asn and His residues, which are conserved through vertebrates. Consistent with its function, GOAT mRNA is largely restricted to stomach and intestine, the major ghrelin-secreting tissues. To further …
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Adaptive metabolic gene clusters as toolkits for chemical innovation: Investigation of the origin of the avenacin gene cluster for synthesis of defense compounds in oats
… P450 51s, Clade 1A serine carboxypeptidase-like acyltransferases, Class I O-methyl transferases and Group L glycosyltransferases in monocots. Phylogenetics analyses show that these gene families evolve via duplication-neofunctionalisation, facilitated by gene GC content and exon-intron structures …
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Roles of Phospholipase A/Acyltransferase Enzymes in Membrane Remodelling and Organelle Degradation
… is coordinated by phospholipase A (PLA) and acyltransferases (AT). PLA/AT are a family of 5 enzymes evolutionarily conserved in vertebrates. Recently, they have been implicated in processes from organelle degradation during lens differentiation to host immune responses against Toxoplasma …
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Identification of acyl transferases responsible for the diverse hydroxycinnamoyl chemical space in bidens pilosa
… regions that are found in already published acyltransferases, with the highly conserved DFGWG motif present in all transcripts identified herein. The second motif, the HXXXD motif showed a single amino acid variation from gene to gene, with HQT1 and HQT2 showing HTLSD motif and HQT3 having …
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Regulation of The Expression and Lysine Acetylation of Pro-Inflammatory Molecules By Lipid-Modifying Enzyme (LPCAT2) in RAW264.7 Cells
Lysophosphatidylcholine Acyltransferases (LPCATs) are Lipid modifying enzymes that significantly regulate changes in the glycerophospholipid components of the macrophage membrane phospholipid bilayer. LPCAT2 is a subtype of LPCAT that regulates inflammatory responses to infection by …
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Develoment and Application of Chemical Strategies to Study Protein Fatty-Acylation in Eukaryotes
… specificity among the highly redundant palmitoyl acyltransferases (PATs) remains unresolved. To study the regulation of PATs and palmitoylomes, I developed bioorthogonal chemical strategies for improved analysis of dynamic palmitoylation in mammalian cells. I showed that alkyne-functionalized …
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Characterization of activation tagged potato (Solanum tuberosum L.) mutants
… mutant, two potato genes, encoding an Acyl-CoA N-acyltransferases (NAT) superfamily protein, and a predicted major facilitator superfamily protein (MFS) were identified and overexpression lines Bintje/35S::NAT1 and Bintje/35S::PMT1 were created for recapitulation of the nikku mutant phenotype. …
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Essential Function of Ghrelin in Chronic Starvation
Ghrelin, an octanoylated peptide hormone secreted from the stomach, stimulates the release of growth hormone (GH) from the pituitary. Ghrelin O-acyltransferase (GOAT) is the enzyme required for the attachment of octanoate to serine-3 of ghrelin, a step essential for making active ghrelin. In this …
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Structural Investigations of Porcupine and Dispatched-1 in Wnt and Hedgehog Morphogen Biogenesis
Here, I present the cryo-EM studies in two key developmental signaling pathways, Hedgehog (HH) and Wnt signaling pathways. HH signaling is vital for metazoan development, and its ligand is secreted by a cell surface protein named Dispatched-1 (DISP1). I report the cryo-EM structure of the DISP1 in …
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Mechanisms of Genome Buffering and Cell Fate Coordination in Adult Tissue Homeostasis
Self-renewal competency of adult stem cells is essential for tissue homeostasis. The corruption of genes essential for genome preservation or for niche-stem cell interactions frequently results in loss of stem cell viability and disease. The two components of my thesis focus on understanding adult …