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Showing 1 to 20 of 33 for “"Acyl Carrier Protein"”.
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Characterization of the Escherichia Coli Acyl Carrier Protein Phosphodiesterase
The activity of AcpH was also examined in relation to the toxicity of N-pentylpantothenamide, an analog of the CoA precursor pantothenic acid. This analog is known to be a substrate for three CoA synthetic enzymes, resulting in a CoA analog with a metabolically inactive prosthetic group …
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Demonstration of novel enoyl-acyl carrier protein reductases in Vibrio cholerae
… in this relatively conserved pathway. The enoyl-acyl carrier protein (ACP) reductase catalyses the last step in the elongation cycle of fatty acids. It is not only a key enzyme in the pathway, but also the most diverse enzyme across bacterial species and the target of many antimicrobial agents. …
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The Role of Acyl Carrier Protein Isoforms in Determining Plant Lipid Content and Composition
Acyl carrier protein (ACP) is a small protein that is a critical cofactor for fatty acid biosynthesis in all organisms. ACP carries growing acyl chains through all of the enzymatic steps in fatty acid biosynthesis. Acyl-ACP's are also the substrate for stearoyl-ACP desaturase and plastid …
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An examination of structural properties and prosthetic group turnover of Escherichia coli acyl carrier protein
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:06:24Z Item is restricted indefinitely.
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Functional Studies of Acyl Carrier Protein and the Phosphopantetheinyl Transferase AcpT in Escherichia Coli K-12
Overexpression of AcpT was reported to suppress an E. coli K-12 mutant that has a temperature-sensitive phenotype and lipid A biosynthetic defect. I have mapped the mutation responsible for the lipid A biosynthetic defect and temperature-sensitive phenotype of this strain and have further …
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STRUCTURE DETERMINATION, BIOCHEMICAL CHARACTERIZATION, AND PROTEIN ENGINEERING OF THE ACYL CARRIER PROTEIN DOMAIN FROM 6-DEOXYERYTHRONOLIDE B SYNTHASE
… containing multiple enzymatic domains, including acyl carrier protein (ACP). ACPs are involved in chain transfer and chain elongation during polyketide biosynthesis. Structural characterization of all seven ACPs could lead to a better understanding of the domain-domain interaction and the …
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Isolation and characterization of a synthetic gene and a genomic clone encoding acyl carrier protein from Escherichia coli
Acyl carrier protein (ACP) is a required cofactor for the synthesis and subsequent metabolism of fatty acids in Escherichia coli. Previous work has suggested that DNA segments encoding ACP were somehow toxic to E. coli. To investigate this possibility, a synthetic gene encoding ACP was assembled …
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Characterization of cDNA and Genomic Clones for a Palmitoyl-acyl Carrier Protein Thioesterase and an Osmotin-Like PR5 Protein in Gossypium Hirsutum.
… and cognate genomic clones for a palmitoyl-acyl carrier protein (ACP) thioesterase (PATE) and an osmotin-like pathogenesis-related 5 (PR5) protein have been isolated and characterized. PATE is a class B fatty acid thioesterase with specificity for saturated long-chain fatty acids such as …
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Palmitoyl-acyl Carrier Protein Thioesterase in Cotton (Gossypium hirsutum L.): Biochemical and Molecular Characterization of a Major Mechanism for the Regulation of Palmitic Acid Content
… be due in part to the activity of a palmitoyl-acyl carrier protein (ACP) thioesterase (PATE). In embryo extracts, PATE activity was highest at the maximum rate of reserve accumulation (oil and protein). The cotton FatB mRNA transcript abundance also peaked during this developmental stage, …
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Molecular cloning and analysis of the genes for cotton palmitoyl-acyl carrier protein thioesterase (PATE) and Δ-12 fatty acid desaturase (FAD2-3) and construction of sense and anti-sense PATE plasmid vectors for altering oilseed composition of transgenic cotton plants.
… The predicted amino acid sequence of the PATE preprotein has a characteristic stromal-targeting domain and a 63% identity to the Arabidopsis FatB1 thioesterase sequence. A cotton genomic clone containing a 17.4-kb DNA segment was found to encompass a palmitoyl-ACP thioesterase (FatB1) gene. The …
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Lipoic acid assembly on 2-oxoacid dehydrogenases in E.coli
… dependent on the activities of the LipB and LipA proteins and a lipoic acid scavenging pathway catalyzed by the LplA protein. LipB is responsible for octanoylation of the E2 components of 2-oxoacid dehydrogenases to provide the substrates of LipA, a SAM radical enzyme that inserts two sulfur atoms …
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Biosynthetic and Chemical Investigation of Lipid II-Binding Antimicrobials.
… of minimally complex analogs, we determined non-proteinogenic amino acids and the N-acyl fatty acid moiety are essential for bioactivity. For the second project, we investigated a conserved mechanism of action for phylogenetically-related natural products within the lasso peptide subfamily. This …
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UNCOVERING THE MOLECULAR BASIS FOR FATTY ACID SYNTHASE PRODUCT SPECIFICITY: STRUCTURAL AND KINETIC EVALUATION OF THIOESTERASE I AND THIOESTERASE II
… expansion, post-translational modification of proteins, and intracellular signalling. FASN primarily generates palmitate (C16) and to a lesser extent stearate (C18), and this strict product distribution is dictated by the acyl chain-length selectivity of the endogenous thioesterase (TE1) …
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Lipoic acid protein ligases in Plasmodium spp.
… the cofactor occurs in the apicoplast. Octanoyl-[acyl carrier protein]: protein N-octanoyltransferase (LipB) catalyses the attachment of octanoyl-acyl carrier protein (octanoyl-ACP) to the PDH and lipoic acid synthase (LipA) then catalyses the insertion of two sulfurs into the octanoyl-chain to …
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Structural Basis for Protein Recognition, Acyl-substrate Delivery, and Product Release by ACP in the Biosynthesis of Lipid A
<p>Acyl-carrier-protein (ACP) is the principal transporter of fatty acids, coordinating acyl-transfer among a vast network of diverse enzymes and biochemical processes. ACP association with protein partners is thought to be exceedingly transient. This paradigm has posed challenges for understanding …
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Post-Translational Modification With Lipoic Acid in Escherichia Coli
… the specific lysine residues of the apoprotein subunits of these complexes. The first pathway identified involves the lplA (lipoate-protein ligase) gene product, which utilizes free lipoic acid found in the environment to modify apoproteins. A second pathway was known to be dependent on …
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Investigating intermediates in 6-methylsalicylic acid biosynthesis
… conditions. Attempts were made to express the acyl carrier protein domain from 6-MSAS to overcome these problems. These experiments represent the first use of the non-hydrolysable analogue methodology in a Type I iterative polyketide synthase and provide a framework for future experiments …
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A genomics-led approach to deciphering heterocyclic natural product biosynthesis
… formation on an enzyme-bound N-formylglycylacyl-intermediate, and its deletion abolished phthoxazolin production. In vitro reconstitution of the early steps of phthoxazolin biosynthesis was attempted to validate the role of PhoxP, but was unsuccessful. Furthermore, Orf3515, a putative …
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Mechanistic Insights into MftR-Dependent Regulation of the Redox Cofactor Mycofactocin
… discovered.</p> <p>While MFT biosynthetic proteins have been extensively characterized, the physiological conditions that require MFT is not well-understood. To gain insights into the regulation of MFT in <em>Mycobacterium smegmatis</em> mc2155, we investigated the DNA-binding and …
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