Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Actins"”.
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The role of PADs in the biogenesis of prostate cancer microvesicles which play a dysfunctional role on DC biology and the therapeutic potential of skeletal muscle microvesicles on prostate cancer cells
… PAD expression and deimination of cytoskeletal-actins is increased. Inhibiting the enzyme with pan-PAD inhibitor chloramidine, abrogated the deimination of cytoskeletal actins as well as reduced the release of MVs. Furthermore, combining chloramidine with anticancer drug methotrexate increased …
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A Molecular Basis for Divergent, Unstable Actin Filaments in Toxoplasma gondii
… assays. Phalloidin binding to parasite actins rescued the short filaments demonstrating that although TgACTI naturally forms short, unstable filaments on its own, it is capable of forming longer, conventional filaments when stabilized. Molecular docking was used to identify divergent …
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Characterizing the Effects of Jasplakinolide on Mesostoma Ehrenbergii Primary Spermatocytes to Study the Role of Actin during Chromosome Movement
My thesis investigated actins involvement in chromosome movement and function in the spindle. Generally, its thought that microtubules drive chromosome movement. However, when microtubules are depolymerized using nocodazole in Mesostoma ehrenbergii, chromosomes continue to move. To determine what …
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Actin-like filament A (AlfA) and AlfA-driven plasmid segregation
… A comparison of AlfA structure with other actins revealed a unique nucleotide binding conformation, which causes AlfA to remain stable after nucleotide hydrolysis. In addition to the filament structure, we reconstituted the segresome (centromeric DNA-adaptor protein) complex in vitro and …
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Analysis of the role of Drosophila IMP in actin mRNA localization and cell migration
… cells. mRNA in situ for the two cytoplasmic actins in Drosophila (actin42A and actin5C) shows that both are localized to the front of the migrating border cell cluster as is IMP protein. Border cells mutant for either actin42A or actin5C are able to perform normal migration supporting the …
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Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective
T cell activation occurs through interaction with an antigen-presenting cell (APC). Upon activation, signaling ensues with the coordination of dozens of diverse signaling molecules in space and time, a feature of cell signaling we call ‘spatiotemporal patterning’. We performed a systems-scale …
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The Molecular Basis of Tissue Elasticity and Force Balance During Drosophila Gastrulation
The mechanics of folding any material rely on two things: the physical forces forming the fold and the material properties of the substance being folded. When working in biological tissue such as an early Drosophila embryo, there is no existing way to directly measure morphogenetic forces, and the …
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Single Molecule-Based Computer Vision Analysis of Cortical Actin-Plasma Membrane Protein Interplay
The file named "NGO-PRIMARY-2025.pdf" is the primary dissertation file. The file named "1745621082149-Supplementary Video.zip" is a zipped file folder that contains 10 movie files (MP4 format), and a description of each video is included in Appendix B of the primary file (pages 148-149).
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Direct Redox Regulation of F-Actin Assembly and Disassembly by MICAL
How guidance cues present outside of cells exert their precise effects on the internal actin cytoskeleton is poorly understood. Such effects are critical for diverse cellular behaviors including polarity, morphology, adhesion, motility, process elongation, navigation, and connectivity. Semaphorins, …
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Direct Redox Regulation of F-Actin Assembly and Disassembly by MICAL
How guidance cues present outside of cells exert their precise effects on the internal actin cytoskeleton is poorly understood. Such effects are critical for diverse cellular behaviors including polarity, morphology, adhesion, motility, process elongation, navigation, and connectivity. Semaphorins, …
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Regulation of Mical Redox Post-Translationally-Driven F-Actin Cytoskeletal Dynamics
The actin cytoskeleton is critical for multiple diverse cellular behaviors, including the ability of an axon to form, extend, navigate, and synapse with its target. Therefore, an important goal is to understand the mechanisms that regulate it. We have been studying one of the largest families of …
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Biochemical Reconstitution and Functional Characterization of the WAVE Regulatory Complex
Members of the Wiskott-Aldrich syndrome protein (WASP) family (WASP, N-WASP, WAVE 1-3) have a central role in the transmission of the extracellular signals to the actin cytoskeleton. These proteins use their C-terminal VCA domain to stimulate the actin-nucleating activity of Arp2/3 complex in …
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Understanding the Role of Loner in Myoblast Fusion
The general metadata -- e.g., title, author, abstract, subject headings, etc. -- is publicly available, but access to the submitted files is restricted to UT Southwestern campus access and/or authorized UT Southwestern users.
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Spatiotemporal Control of Actin Cytoskeletal Machinery in Cells: Multivalency Mediated Protein Clustering at the Plasma Membrane and Optogenetic Tool Development
Precise regulation of actin cytoskeleton dynamics is crucial for numerous cellular processes. My thesis work has demonstrated two different mechanisms that cells use to achieve spatiotemporal control of actin assembly -- multivalency mediated phase separation and subcellular activation of actin …
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Transcriptional Regulation of the BOMBYX MORI A3 Cytoplasmic Actin Promoter in Insect and Mammalian Cells
<p>In this study we have analyzed the activity of the silkworm A3 cytoplasmic actin promoter in homologous insect and heterologous mammalian expression systems. To study the expression of the A3 actin promoter in insect cells we have isolated and characterized a stably transformed Bombyx mori cell …
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Physical Studies of Actin Nucleation and Conformational Dynamics
Actin is a 42 kilodalton ATPase that exists ubiquitously in eukaryotic cells. Unlike other ATPases, however, actin, under suitable conditions, can polymerize, forming helical filaments. Cells, in orchestrating their myriad cellular processes, utilize actin's intrinsic capacity to polymerize, but do …
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Investigating the Function of an Actin Nucleation-Promoting Factor N-WASP in Mammalian Myoblast Fusion
Pages 61-89 are misnumbered as pages 75-103, and pages 90-100 are misnumbered as pages 102-112.