Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"Actinin"”.
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Evolutionary Dynamics of the Alpha-actinin Gene Family
The alpha-actinin gene family is a member of the spectrin superfamily of proteins. Other members of this superfamily include the a- and ~-spectrins, ~heavy spectrin and dystrophin. Each member of this superfamily has a unique actin cross-linking distance consequent to the specific number of …
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alpha-Actinin-4 and the podocyte: Implications for focal segmental glomerulosclerosis
… by expressing a high-affinity variant of alpha-actinin-4 (K256E) in a podocyte-specific manner. Transgenic mice display podocyte damage, subsequent loss of serum proteins into the urine (proteinuria), and glomerular sclerosis, similar to that observed in human patients. In cultured podocytes, …
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Ca²⁺ and phosphoinositides regulations in α-actinin -4 F-actin binding.
α-actinin-4 is a non-muscle isoform of α-actinin that belongs to the spectrin superfamily. It comprises three functional regions: an N-terminal actin-binding region that consists of two calponin homology (CH) domains, a central region that consists of four copies of the spectrin-like repeat domain …
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A study on the modulation of alpha-actinin and filamentous actin
… extracellular matrix. Here I demonstrate that α-actinin-4, a well-known actin crosslinking protein and long believed to be the primary actin bundling protein for contractile actin arrays, actually inhibits bundling of actin filaments into stress fibers in epithelial cells. I show that α-actinin-4 …
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Dynamic viscoelasticity of actin networks cross-linked with wild-type and mutant [alpha]-actinin-4
The actin cross-linker [alpha]-actinin-4 has been found indispensable for the structural and functional integrity of podocytes; deficiency or alteration of this protein due to mutations disturbs the cytoskeleton and results in kidney disease. This thesis presents rheological studies of in vitro …
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Dynamics of Alpha5 Integrin, Paxillin, and Alpha-Actinin During Formation and Disassembly of Adhesions in Migratory Cells
… role of the alpha5 integrin, paxillin, and alpha-actinin in the migration process. The alpha5 integrin as well as the actin-binding protein alpha-actinin are membrane-localized in actively protruding regions of the cell. The alpha5 integrin departs from protrusive regions, as well as from …
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An investigation of the role of ACTN1 in platelet formation and of platelet function in myeloma
… prothrombotic state in Multiple Myeloma (MM). Actinin-1 is a widely-expressed actin binding protein but mutations have a tissue-specific impact, causing CMTP, characterised by low platelet count and large platelet size. It was previously shown that mutations in the actin-binding domain of …
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Conformational changes in actinin-type actin binding domains: probing actin-induced structural dynamics in dystrophin and utrophin using EPR spectroscopy.
… binding domains, including an N-terminal "actinin-type" actin binding domain (ABD).In addition to dystrophin and utrophin, actinin-type ABDs are found in a large number of proteins. Nonetheless, the actin binding mechanism remains poorly understood: x-ray crystallography and electron …
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Localization of Cytoskeletal Elements in Teleost Retinal Pigment Epithelium
… the actin binding region of human sarcomeric a-actinin. Recent Studies have suggested that the G.3.5 antigen may be a novel isoform of a-actinin, and may function to cross-link [Fs to MFs. In this study, I have used epifluorescence labeling techniques on cryosectioned bluegill RPE that have …
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miR-322/503 Cluster Drives Cardiac Differentiation by Inhibiting CUG-binding Protein 1
… to mimic the natural course in mESCs led to α-actinin expression and significant increases of cardiac transcription factors (Tbx5, Mef2C, Nkx2-5 and α-MHC); and (3) inhibitors of miR-322 and miR-503 significantly reduced expression of α-actinin and the above cardiac TFs. Remarkably, miR-322/503 …
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Serine 910 Phosphorylation of Focal Adhesion Kinase Is Critical for Costamere Assembly
… This interaction was important in stabilizing α-actinin within the protein complex, as evident by a decrease in <sub>k</sub>FRAP for α-actinin-YFP. The S910A-FAK mutant also blocked ET-1 induced NRVM spreading and cyotoskeletal reorganization. Finally, we found that FAK was serine-phosphorylated …
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Mapping the actin and actin binding proteins interactions : from micromechanics to single molecule force spectroscopy
… different ABPs: filamin, a crosslinker, and a-actinin, a bundler. We measured rupture forces of 28-73 pN and 30-56 pN for filamin/actin and a-actinin/actin respectively, suggesting that the former is a slightly stronger interaction. Moreover, since no ABP unfolding events were observed at our …
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Investigation of the mechanisms underlying the effects of hyperglycaemia on cardiac structural and electrical remodelling
… were positive for cardiac proteins troponin T, α-actinin 2, connexin 43, sarco-endoplasmic reticulum calcium ATPase 2 (SERCA 2) and α- and β-myosin heavy chain. Hyperglycaemia decreased the number of beating EBs, their beating rate, and their amplitude of contraction. It also decreased the calcium …
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Strukturen der Kraftübertragung im quergestreiften Muskel : Protein-Protein-Wechselwirkungen und Regulationsmechanismen
… an die beiden Liganden N-RAP und alpha-Actinin. Die molekulare Grundlage der HCM-verursachenden Mutationen im MLP-Gen könnte folglich eine Veränderung der Homöostase im ternären Komplex MLP – N-RAP – alpha-Actinin sein. Die Expressionsdaten eines neu generierten monoklonalen …
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The zebrafish maternal factor pollywog is required for yolk syncytial layer morphogenesis
… in this thesis is work highlighting the alpha-actinin gene family in zebrafish. alpha-actinins are actin microfilament crosslinking proteins. Vertebrate actinins fall into two classes: the broadly-expressed actinins 1 and 4 ( actn1 and actn4 ) and muscle-specific actinins, actn2 and actn3 . …
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Effects of cross-link and myosin motor concentrations on active muscle gel contraction time and extent
… the concentration of myosin motors and alpha-actinin crosslinks.
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The effects of force on actin-myosin ATPase activity
… imposed on a sliding actin filaments using alpha-actinin, and the corresponding effects of load were determined using a novel experimental and theoretical protocol. Our results show that mechanical loads decrease both actin-myosin attachment and detachment kinetics during actin sliding in skeletal …
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Tyrosine phosphorylation and cytoskeletal tension regulate the release of fibroblast adhesions
… cells appear contracted with talin, $\alpha$-actinin and vinculin localized diffusely throughout the cell. Integrin containing tracks of variable intensity outline the regions where cells had resided before they detached from the substratum. At lower ATP concentrations (0.5-5mM) the cells …
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Junction Specificity and Transmembrane Linkages of Integrins
… architecture was positive for $\alpha$-actinin. Together, the data indicate the P4B2 antigen is part of a unique GAPDH containing protein complex that may be involved in reinforcement of established cytoskeletal structures.
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