Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Actin depolymerization"”.
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Actin's role in signaling light-induced phase shifts in the mammalian circadian clock
The actin cytoskeleton is a critical part of intracellular structure, responsible for the general shape and integrity of all cells. New insights into the dynamic nature of the cytoskeleton describe additional roles for actin, including synaptic plasticity in the hippocampus, regulation of hormone …
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Alteration of prenylation: Effect on neurite outgrowth and Rho GTPase signaling
… cofilin phosphorylation with effects on actin filament content since cofilin is an actin depolymerizing agent. We use western blot analyses and immunocytochemistry to assess phosphorylated and total cofilin and find no significant deviations from control conditions. However, the amount of …
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Mitotic Exit Control in Budding Yeast: Regulators and Dynamics
… given a block of cell cycle progression by actin depolymerization. Using genetic manipulations, we demonstrate that this oscillator can operate in free-running cell cycles even without undegradable Clb2. The Cdc14-Cdh1-Cdc5 negative feedback is the primary mechanism driving this release …
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Calcium, actin and the spectrin skeleton: Responses to swelling and shrinking perturbations.
… incubated in cytochalasin, a drug that promotes actin depolymerization, do not undergo VLD recovery. Microscopy studies into how neuronal F-actin responded to osmotic perturbations and how the F-actin is related to VLDs were initiated. In Lymnaea neurons that had swelled then reshrunk to form …
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Pathological Upregulation of a Calcium-Stimulated Phosphatase, Calcineurin, in Two Models of Neuronal Injury
… dephosphorylation and activation of cofilin, an actin-depolymerizing factor. Calcineurin-activated cofilin induced an increase in actin depolymerization, a mechanism shown to cause spine loss in other models. Finally, via Golgi impregnation, we demonstrated that status epilepticus-induced spine …
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Organization of Postsynaptic Proteins via the Neuronal Cytoskeleton
… were treated with latrunculin A to depolymerize actin, vincristine to depolymerize microtubules, or Triton X-100 to extract soluble proteins. Depolymerization of F-actin led to a decrease in both the number of synaptic NMDAR1 clusters and the number of GluR1-labeled spines. On GABAergic neurons, …
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A biochemical investigation of actin disassembly mechanisms
The dynamic nature of the actin cytoskeleton enables the rapid shape changes that are necessary for processes such as wound healing, motility and division of cells. Disassembly of actin filaments is extremely critical for the reorganization of cell shape. The cell possesses several factors that …
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Cationic amphiphilic drug-induced autophagosome accumulation is due to autophagosome sequestration within vimentin intermediate filament networks resulting in prolonged autophagosome half-life
… revealed U18666A treatment affected</p> <p>F-actin and vimentin, but not microtubules. Rhodamine-phalloidin staining</p> <p>indicated U18666A induced F-actin depolymerization. Actin repolymerized</p> <p>when cultures were shifted to leucine-starvation medium. However, this effect</p> <p>did …