Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 42 for “"Actin cytoskeletal"”.
-
Regulation of Mical Redox Post-Translationally-Driven F-Actin Cytoskeletal Dynamics
The actin cytoskeleton is critical for multiple diverse cellular behaviors, including the ability of an axon to form, extend, navigate, and synapse with its target. Therefore, an important goal is to understand the mechanisms that regulate it. We have been studying one of the largest families of …
-
Functions of Phosphatidylinositol 4-Phosphate 5 Kinases in Actin Cytoskeletal Regulation During Phagocytosis
… segregated PIP2 pools are generated. The actin cytoskeleton is dynamically remodeled during Fcg receptor (FcgR)-mediated phagocytosis in a PIP2-dependent manner. I investigated the role of PIP5Kg and a isoforms, which synthesize PIP2, during phagocytosis. PIP5Kg-/- bone marrow-derived …
-
Role of Cofilin, an Actin Cytoskeletal Protein, in Ischemic Conditions: Potential Therapeutic Target for Ischemic Stroke
… both degenerative and regenerative mechanisms. Actin cytoskeleton changes play a key role in mediating these responses. Cofilin, an actin cytoskeletal severing protein is known to be involved in early stages of apoptotic cell death. Evidence supports its intervention in progression of disease …
-
Spatiotemporal Control of Actin Cytoskeletal Machinery in Cells: Multivalency Mediated Protein Clustering at the Plasma Membrane and Optogenetic Tool Development
Precise regulation of actin cytoskeleton dynamics is crucial for numerous cellular processes. My thesis work has demonstrated two different mechanisms that cells use to achieve spatiotemporal control of actin assembly -- multivalency mediated phase separation and subcellular activation of actin …
-
The Effect of Microenvironmental Cues on Adipocyte Cytoskeletal Remodeling
… on the adipocyte mechanical response, including actin cytoskeletal remodeling. This dissertation aims to develop an in vitro model of healthy/diseased AT to explore the effect of microenvironmental cues on adipocyte function and actin cytoskeletal remodeling. The first aim is to study (1) the …
-
Direct Redox Regulation of F-Actin Assembly and Disassembly by MICAL
… exert their precise effects on the internal actin cytoskeleton is poorly understood. Such effects are critical for diverse cellular behaviors including polarity, morphology, adhesion, motility, process elongation, navigation, and connectivity. Semaphorins, for example, are one of the largest …
-
Direct Redox Regulation of F-Actin Assembly and Disassembly by MICAL
… exert their precise effects on the internal actin cytoskeleton is poorly understood. Such effects are critical for diverse cellular behaviors including polarity, morphology, adhesion, motility, process elongation, navigation, and connectivity. Semaphorins, for example, are one of the largest …
-
Invadopodia Formation in Breast Cancer
… of the cancer cells. Cell invasion requires actin cytoskeletal reorganisation and cells are thought to utilise specialised membrane protrusions termed invadopodia. Rho family GTPases Rac and Cdc42 through interaction with effector proteins p21-activated kinases (PAKs) are known to regulate …
-
HIV-1 Envelope-Induced Signaling Mediates Actin Cytoskeleton Rearrangements Necessary for Fusion and Entry
… primary receptor CD4. This interaction promotes actin cytoskeletal rearrangements in the target membrane that bring the chemokine coreceptor, CCR5 or CXCR4, into close proximity for binding and induces conformational changes in gp120 that allow it to couple to the coreceptor. Formation of the …
-
Influence of rearrangement of actin cytoskeleton on the overall material properties of ATDC5 cells during chondrogenesis
… in 3D for 12 days and fluorescent images of the actin cytoskeletal structure of the cells were obtained. A major reorientation of the cytoskeletal structure (from stress-fiber to punctate appearance) was observed. This data was then used to construct a Finite Element model of cell. The cell was …
-
Characterization of Redox Active Natural Compounds in Chlamydia Infection
… inclusion development, processes dependent on F-actin/cytoskeletal reorganization.
-
Regulation of Net1A Subcellular Localization By The Small Gtpase Rac1
… is required to stimulate RhoA activation, actin cytoskeletal reorganization, and oncogenic transformation. However, regulatory mechanisms controlling the extranuclear localization of Net1 have not been identified. In this study, we have addressed the regulation of Net1A isoform localization …
-
Determining the differential roles of the dock family of GEFs in drosophila development
… of the Dock-A subfamily is Rac, which modulates actin dynamics. However, the in vivo GTPase downstream of the Dock- B subfamily remains unclear. Herein we show that the roles of Spg and Mbc are not redundant in the Drosophila somatic muscle, central nervous system (CNS), or the dorsal vessel …
-
Effects of hypertension and aging on vascular smooth muscle cell contribution to reconstituted aortic tissue stiffness
… tissue; and following treatment with an actin cytoskeletal disrupter (cytochalasin D), which effectively removed the cellular contribution to stiffness. The cellular contribution was found from the difference between these stiffnesses. Aging and hypertension sharply increased VSMC …
-
Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions
… and local alteration in the presynaptic actin cytoskeleton. BMP acts during development to provide competence for rapid synaptic growth by regulating the levels of the Rho GEF trio, a transcriptional output of BMP-Smad signaling. In a parallel pathway, we find that the BMP type 11 …
-
P16/INK4a controls the morphology program associated with cellular senescence
… p16 inactivation results in rearrangement of the actin cytoskeleton and the loss of the extensive web of actin stress-fibers typically present in senescent cells. <br>Young dividing cells with inactivated p16 do not attain the classical senescent phenotype if propagated in culture, while they do …
-
The receptor tyrosine phosphatase Ptp69D and the receptor tyrosine kinase Pvr in Drosophila nervous system development
… cell adhesion, and Abl is a known regulator of actin cytoskeletal dynamics. Removing a single dose of the EGF receptor or Ras85D suppressed the Ptp69D dominant negative phenotype. Interestingly, the cytoplasmic tyrosine kinase PR2 binds Ptp69D, and the C. elegans homolog of PR2 has been found to …
-
Notopleural Mutations Enhance Defects In Imaginal Disc Epithelial Morphogenesis And Macrochete Elongation Associated With Mutations in the Stubble-Stubbloid Locus
… analysis indicate a role for Stubble in actin cytoskeletal dynamics and cell shape changes in developing epithelia and bristles. Previously reported genetic interactions between Stubble and the Rho1 signaling pathway suggest Stubble influences actin cytoskeleton dynamics in developing …
-
Investigating Mechanical Strain-Induced Phenotypic Changes on Prostate Cancer Cell Toward Metastasis Using a Three-Dimensional <i>In-Vitro</i> Model
… application). The effect of mechanical strain on actin cytoskeletal organization was first investigated using a time lapse study. Following this, a larger data set was collected for further analyze cell elongation and actin polymerization. Finally, the effect of mechanical strain on cell invasive …
-
Regulation of RhoA Activation and Actin Reorganization by Diacylglycerol Kinase
Rho GTPases are critical regulators of actin cytoskeletal dynamics. The three most well characterized Rho GTPases, Rac1, RhoA and Cdc42 share a common inhibitor, RhoGDI. It is only recently becoming clear how upstream signals cause the selective release of individual Rho GTPases from RhoGDI. For …
Page 1 of 3