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Showing 1 to 12 of 12 for “"Actin bundling"”.
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Human FRG1 is an Actin-Bundling and a RNA-Associated Protein with Distinct Subcellular Localizations
… cytoplasmic pool that is dependent on an intact actin cytoskeleton for its localization and we demonstrate FRG1 itself is an actin binding protein. These data provide the first biochemical activities for human FRG1 and indicate that FRG1 dynamically shuttles between the nucleus and cytoplasm and …
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A study on the modulation of alpha-actinin and filamentous actin
Cells use actin to provide structure and stability to the membrane, to drive motility, and to adhere to, pull on, and reorganize the extracellular matrix. Here I demonstrate that α-actinin-4, a well-known actin crosslinking protein and long believed to be the primary actin bundling protein for …
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Dynamics and statics of actin assemblies
… where a 60 [mu]m-long crystalline bundle of actin filaments, tightly cross-linked by actin bundling protein scruin, straightens from a coiled conformation and extends from the cell in five seconds. This spring-like mechanism represents a third type of actin-based motility that is distinctly …
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Contributions to the analysis of proteins
… the structure of Homo sapiens fascin-1, an actin-binding protein that is present predominantly in filopodia, is examined and described in detail. Application of a sequence conservation analysis to the protein indicates highly conserved surface patches near the putative actin-binding domains …
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Redefining endothelial progenitor cells using a proteomics approach
… resulted in the targeted reduction of the actin-bundling protein LCP-1, facilitating the adhesive and migratory ability of mononuclear cells. These findings demonstrate the advantage of using an unbiased proteomic approach to assess cellular phenotypes and advise caution in attributing the …
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Insights into protein function from evolutionary and conformational dynamics
… in a class of proteins that bundle filamentous actin (F-actin)?" Through simulation we demonstrate that cooperative F-actin bundling tends to strengthen bundles by driving the formation of cross-links between neighboring filaments while depleting F-actin binding sites that are occupied but not …
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Ca²⁺ and phosphoinositides regulations in α-actinin -4 F-actin binding.
α-actinin-4 is a non-muscle isoform of α-actinin that belongs to the spectrin superfamily. It comprises three functional regions: an N-terminal actin-binding region that consists of two calponin homology (CH) domains, a central region that consists of four copies of the spectrin-like repeat domain …
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Multi-Modal Regualtion of Actin Networks
<p>Eukaryotic cells employ the actin cytoskeleton to maintain cell shape, support motility, and sense and respond to external mechanical stimuli. An intricate network of actin filaments constitutes the foundation of tissue architecture by forming key cellular structures including the muscle fiber, …
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The Structural and Biochemical Characterization of Salmonella Host Specificity Determinant Gifsy-2 Gene E
… many different effector proteins capable of interacting with and altering numerous biological pathways in the host – enabling host invasion and intracellular survival as well as dissemination and transmission. We used X-ray crystallography to characterize Gifsy-2 Gene E (GtgE), an effector …
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Actin Cable Function and Regulation in the Budding Yeast, Saccharomyces cerevisiae
… chapters, I describe factors underlying actin cable dynamics and assembly in the budding yeast, S. cerevisiae. First, I examined the role of type II myosin and a tropomyosin isoform in retrograde actin flow (Chapter II). In yeast and other cell types, actin undergoes retrograde or …
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Structural Mechanisms of F-actin Mechanochemical Regulation and Flexible Network Assembly
<p>The actin cytoskeleton integrates physical and chemical cues from a cell's environment to instruct its behavior, coordinating processes including cell migration, organelle dynamics, and endocytosis. Actin-binding proteins (ABPs) specify actin filament (F-actin) organization to build micron-scale …
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Charge and membrane curvature induced transitions in biomolecular systems: from actin bundles to cell-penetrating peptides to Bcl-2 cell death regulation in the mitochondria
Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2014-02-01T11:00:26Z Item was in collections: Graduate Theses and Dissertations at Illinois (ID: 204) Dissertations and Theses - Physics (ID: 445) No. of bitstreams: 2 Lai_GheeHwee.pdf: 9845710 bytes, checksum: …