Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Actin binding domain"”.
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Functional and structural studies of a small F-actin binding domain
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1992.
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A study on the modulation of alpha-actinin and filamentous actin
Cells use actin to provide structure and stability to the membrane, to drive motility, and to adhere to, pull on, and reorganize the extracellular matrix. Here I demonstrate that α-actinin-4, a well-known actin crosslinking protein and long believed to be the primary actin bundling protein for …
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Conformational changes in actinin-type actin binding domains: probing actin-induced structural dynamics in dystrophin and utrophin using EPR spectroscopy.
… is a lack of functional dystrophin, a large multidomain protein. Dystrophin is normally expressed in muscle, where it links the extracellular matrix to the cortical actin cytoskeleton via a complex of associated proteins. Dystrophin, and its autosomal homologue utrophin, connect with the actin …
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An investigation of the role of ACTN1 in platelet formation and of platelet function in myeloma
… prothrombotic state in Multiple Myeloma (MM). Actinin-1 is a widely-expressed actin binding protein but mutations have a tissue-specific impact, causing CMTP, characterised by low platelet count and large platelet size. It was previously shown that mutations in the actin-binding domain of …
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Characterization of Smooth Muscle Myosin Light Chain Kinase Using Crystallization and Transient Kinetics
… In this project, the crystallization of the actin-binding domain of MLCK (called N1-75) with glutathione S-transferase (GST) was attempted in order to provide such a model. Polyethylene glycol and ammonium sulfate, within a pH range of 6.5 to 8.0, seemed to be the most promising precipitants …
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Insights into the structures and dynamics of the pathogen secreted effectors AVR3A11 and tarp through the application of NMR spectroscopy
… bacterial e�ector proteins. TARP (translocated actin recruiting protein) is a largely disordered 100 kDa e�ector, common to all chlamydial species, which functions to remodel the host actin cytoskeleton to facilitate the internalisation of the chlamydial cell. Using constructs of TARP comprising …
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Investigating the Structure of the Papain-Inhibitor Complex using SPR and NMR
… of defined loops that play a significant role in binding CPs. Papain, a CP from Carica papaya, complexes with ICP from Leishmania mexicana. Although the individual 3-D structures of ICP and papain have been determined, as of this work, the structure of the papain-ICP complex has only been …
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Characterization of GAR22 function in the regulation of red blood cell differentiation and cell migration
… cells, including BTEB1 (basic transcription binding protein 1/ Krüppel-like factor 9) and the novel TR target gene GAR22 (growth arrest specific gene 2 on chromosome 22). The upregulation of GAR22 in terminally differentiated cells suggests that GAR22 might be involved in regulation of growth …
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Force-dependent changes in alpha-catenin conformation
… cadherin complexes. Mechanical force regulates binding of α-catenin to the actin-binding protein vinculin and to the actin cytoskeleton itself. This regulation is accomplished through distinct conformational changes in the α-catenin central modulatory (M) domain and the C-terminal actin-binding …
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Calcium and actin coordinate egg activation and the metaphase-anaphase transition in Drosophila oocytes
… I provide detailed visualisation of the actin population in the mature oocyte both before and after egg activation, focusing on the cortical actin. I reveal a clear relationship between calcium entry and the cortical actin- In particular, reduction of the cortical actin density or level …