Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 113 for “"Actin Filaments"”.
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On the depolymerization of actin filaments
Actin is one of the most abundant and highly conserved proteins in eukaryotic cells. The globular protein assembles into long filaments, which form a variety of different networks within the cytoskeleton. The dynamic reorganization of these networks - which is pivotal for cell motility, cell …
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A Molecular Basis for Divergent, Unstable Actin Filaments in Toxoplasma gondii
… that is dependent on polymerization of parasite actin. However, in non-motile parasites, the majority of actin is monomeric and filaments only assemble upon initiation of gliding motility. Actin filament turnover is crucial for motility as shown by the detrimental effects of jasplakinolide, an …
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Migration of Nucleocapsids in Vesicular Stomatitis Virus-Infected Cells is Dependent on Microtubules and Actin Filaments
… we show a necessity of both microtubules and actin filaments on nucleocapsid migration during infection and virus assembly.
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The dynamics and regulation of actin filaments in vascular endothelial cells and in a reconstituted purified protein system
… on the cytoplasmic dynamics and distribution of actin monomer and polymer. Proteins that regulate actin cycling control cellular architecture and movement. One method to measure parameters that characterize actin dynamics is photo activation of fluorescence (PAF), which can simultaneously …
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A biochemical investigation of actin disassembly mechanisms
The dynamic nature of the actin cytoskeleton enables the rapid shape changes that are necessary for processes such as wound healing, motility and division of cells. Disassembly of actin filaments is extremely critical for the reorganization of cell shape. The cell possesses several factors that …
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Interactions between the plant Golgi apparatus and the cytoskeleton
… in the cortical cytoplasm and moving on the actin cytoskeleton. The contribution of actin filaments to Golgi body motility in plant has been extensively described, but this actin-centric view has recently been challenged. Emerging evidence suggests that microtubules may contribute to short …
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Determining Molecular Mechanisms of Cell Division in Fission Yeast by Testing Major Assumptions of the Search, Capture, Pull, and Release Model of Contractile-Ring Assembly
… yeast to humans assemble the contractile ring of actin, myosin-II, formin, and other proteins, which constricts to divide the cells. We tested some of the basic assumptions of the search, capture, pull, and release (SCPR) model to explain the mechanism of contractile-ring assembly during …
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Two-Color Fluorescent Analysis of Connexin 36 Turnover and Trafficking – Relationship to Functional Plasticity
… we studied the role of cytoskeletal elements, actin filaments in particular, in Cx36 vesicle trafficking and GJ mobility. Phalloidin labeling showed that thick actin bundles connected all edges of GJ plaques, but actin filaments were rare within. Actin filaments were found associated with …
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The Role of Actin Depolymerizing Factor in Regulating Actin Dynamics in Toxoplasma gondii
… Gliding and invasion are dependent on parasite actin filaments, yet parasite actin is mostly non-filamentous. Filaments have been detected only transiently during gliding, suggesting that parasite actin filaments are rapidly assembled and disassembled during gliding motility. Little is known …
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Combined optical trapping and single molecule fluorescence to study the force-dependent binding kinetics between filamentous actin and its partners
Actin filaments are a major constituent of the cytoskeleton in most eukaryotic cells. They function as a connection between the cell body to the focal adhesions in order to transmit forces into and out of the cell. During the force transduction process, many proteins bind to actin filaments in …
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Multi-Modal Regualtion of Actin Networks
<p>Eukaryotic cells employ the actin cytoskeleton to maintain cell shape, support motility, and sense and respond to external mechanical stimuli. An intricate network of actin filaments constitutes the foundation of tissue architecture by forming key cellular structures including the muscle fiber, …
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The S. cerevisiae calponin homologue SCP1 regulates stability and organization of the actin cytoskeleton
… transgelins are members of a conserved family of actin-associated proteins widely expressed from yeast to humans. While a role for calponin in muscle cells has been described, the biochemical activities and in vivo functions of non-muscle calponins and transgelins are largely unknown. I have used …
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Engineered Cytoskeletal Arrays Reveal Mechanisms Of Membrane Transport And Tubulation
… membrane-bound cargos along microtubule and actin filament tracks. Typically, there are multiple actin and microtubule motors attached to the same cargo, which must coordinate to navigate a complex cytoskeletal environment and deliver their cargos to specific locations. We used an …
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SINGLE-MOLECULE STUDY OF THE FORCE-INDUCED ACTIN POLYMERIZATION MEDIATED BY FORMIN
… A LARGE NUMBER OF CELLULAR FUNCTIONS VIA ACTIN CYTOSKELETON. TENSILE FORCES HAVE BEEN PREDICTED TO PROMOTE ACTIN POLYMERIZATION VIA BARBED-END FORMIN PROTEINS AS A NOVEL MECHANISM OF MECHANOTRANSDUCTION. HOWEVER, THIS HYPOTHESIS HAS NOT BEEN WELL CONCLUDED DUE TO THE LACK OF SENSITIVE …
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Novel roles of ADF/cofilins in maintenance of homeostasis in normal and malignant epithelial cells
Actin cytoskeletal regulation is of critical importance for a number of diverse cellular functions, including cell motility, endocytosis, cell division and transcription. Tight regulation of actin is critical for many aspects of cancer biology and in particular invasion and metastasis. ADF/cofilins …
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Computational study of actin morphology and rheology
The cytoskeletal network consisting mainly of actin and actin binding proteins is highly dynamic, provides structural integrity to cells, and plays a central role in a wide range of mechanical and biological functions such as migration and the sensation of external forces. Thus, knowledge of actin …
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Actomyosin Contractility in Nonmuscle Cells
… stresses generated by the action of myosin minifilaments are analyzed via simulation of their motion in idealized computer-generated random, isotropic actin networks and actin bundles. The networks and bundles are generated as random collections of actin filaments in two dimensions with …
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Conformations of semiflexible polymers and filaments
… of semiflexible polymers, such as DNA, actin filaments and carbon nanotubes, strongly depend on their rigidity. Semiflexible polymers are characterized by their persistence length, the definition of which is the subject of the first part of this thesis. Attractive interactions, that …
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