Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Acrosome Reaction"”.
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Regulation of SNARE function during sperm capacitation to promote the acrosome reaction
… to develop a clearer understanding of how the acrosome reaction is regulated during capacitation so that the molecular defects that cause infertility can be identified. I hypothesized that capacitation alters sperm protein phosphorylation promoting soluble N-ethylmaleimide-sensitive factor …
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Molecular Interactions During Fertilization: Sperm -Egg Binding and Regulation of the Acrosome Reaction
… candidate receptor was not identified. The sperm acrosome reaction is an exocytotic event that is initiated when sperm bind to the zona and is required for zona penetration. During the acrosome reaction, the plasma membrane fuses with the outer acrosomal membrane and vesicle contents are released. …
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Re-localization of SNARE proteins in mouse sperm prior to the acrosome reaction
… the outer acrosomal membrane. During the sperm acrosome reaction, the outer acrosomal membrane fuses at hundreds of points with the overlying plasma membrane, resulting in release of the acrosomal contents. We hypothesize that syntaxin and synaptobrevin re-localize within the sperm plasma …
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The Effects Of Equine Spermatozoa/Oocyte Co-Culture On Spermatozoal Acrosome Reaction And Viability
… of oocyte and spermatozoal interactions on acrosome reaction and viability of equine spermatozoa during in vitro fertilization. Three ejaculates were collected from three stallions on an every other day basis. Semen was processed and allowed to incubate in 37⁰ C with 5% CO₂ incubator for 15 …
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Identification of platelet activating factor (PAF) receptor in equine spermatozoa and its role in motility, capacitation and the acrosome reaction
… spermatozoal motility, capacitation, and the acrosome reaction (AR) were evaluated. Treatment of 10 stallion semen samples with 10 â 4 to 10 â 13 M PAF resulted in statistically significant differences in motility and capacitation (r2 = 0.81 and 0.83 respectively). The concentration of PAF, …
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Characterization of the Biological Functions of Human Recombinant Zona Pellucida Protein 3
… assay and immunofluorescence staining of acrosome reaction The results indicated that rhZP3 exhibited an inhibition in the binding assay (HZI 43.6 +/-3.3; n = 9; 30 ng/mL rhZP3) and induction of acrosome reaction (198.6% +/- 77.2% increase from baseline; n = 29; 30 ng/mL rhZP3). It was …
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Choline Acetyltransferase and Carnitine Acetyltransferase Activity in Human Spermatozoa During Capacitation
… and pharmacological induction of the acrosome reaction. A significant increase in both equatorial and midpiece/tail immunoreactivity was observed when using both caffeine and pentoxifylline. The addition of caffeine significantly increased equatorial binding, midpiece/tail binding, and …
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Expression, Isolation and Purification of Human Zona Pellucida Protein 3
… the subsequent triggering of the spermatozoa's acrosome reaction. Studies on the role of this specific protein in the human fertilization process are hampered by the limited amount of available biologically functional proteins.</p> <p>By use of a pcDNA 3.1(+) expression vector, a …
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Structural and functional aspects of sperm after transit through the fallopian tube of the sheep
… include the processes of capacitation and the acrosome reaction. The Fallopian tube has been accepted as the site of completion of these events while their initiation may occur either in the uterus or in the Fallopian tube. The role and/or interaction of the Fallopian tube with sperm to undergo …
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Characterization of the Biological Activities of Recombinant Fusion Protein Green Fluorescent Protein/Human Zona Pellucida Protein 3 (GFP/HZP3)
… protein interacted with human sperm on the acrosome region. In the acrosome reaction assay, fusion protein was proved to be able to induce the sperm acrosome reaction (19.2% ± 3.4% as compared to basal line 10.8% ± 2.3%) at the concentration of 1ug/ml. Therefore, the fusion protein displayed …
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An investigation of the mechanics of an actin spring
… example is the dramatic process involving the acrosome reaction of Limulus sperm, whereby a 60-im long bundle of actin filaments straightens from a coiled conformation to extend out of the cell in five seconds. This cellular engine and the motion it produces represent a third type of …
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Expression and Function of Complexin 1 in Mammalian Sperm Acrosomal Exocytosis
… exocytosis but the sperm vesicle, known as the acrosome, is a single large vesicle whose release has different characteristics than neuronal exocytosis. Upon adhesion to the mammalian egg extracellular matrix, the sperm acrosome is released to allow penetration of the egg coat. How vesicle …
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Characterization of the lipid composition of washed and percoll gradient centrifuged epididymal mouse sperm.
… membrane increases to prepare sperm for the acrosome reaction (AR) and subsequent sperm-egg binding. While cholesterol efflux may be partially responsible for this increase, modification of lipid components, such as remodeling of specific phospholipid (PL) bound unsaturated fatty acyl chains, …
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Measurement of cyclic nucleotides in human sperm: experimental and clinical application
… molecules for capacitation, motility, and acrosome reaction in mammalian sperm. Given their importance in motility and fertilising ability, defective nucleotide function could be a cause of male infertility.<br/><br/>This work was undertaken to establish a method for the extraction and …
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Role of second generation phosphodiesterase inhibitors on mammalian sperm mobility
… results and stimulation of premature acrosome reaction has precluded their clinical use. Advancement in our knowledge have now made it clear that human sperm express several different PDEs and these are compartmentalised at different regions of the cells. By using type-specific …
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Dynamics and statics of actin assemblies
… is a ubiquitous theme across biology. The acrosome reaction of Limulus sperm is a simple example of such a dynamical transformation where a 60 [mu]m-long crystalline bundle of actin filaments, tightly cross-linked by actin bundling protein scruin, straightens from a coiled conformation and …
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Factors causing anterior acrosomal swelling on motile bovine sperm
Swelling of the apical ridge and anterior acrosome of motile bovine sperm was observed using differential interference contrast optics. Transmission electron microscopy indicated that the acrosomal matrix was extended into complex folds and/or projections. Outer acrosomal and plasma membrane …
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In vitro Expression of Human Zona Pellucida Protein 3
… mRNA by reverse transcription-polymerase chain reaction (RT-PCR). The hZP3 cDNA was subcloned into PSK and pREP4 expression vectors. The cDNA of hZP3 was further characterized by restriction mapping, PCR, auto-sequencing and Southern blot analysis by using an internal oligonucleotide probe, and …
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Role of the oviduct environment in sperm storage
… 5 mM creatine supplementation had no effect on acrosome stability or the ability of sperm to undergo the acrosome reaction when induced by calcium ionophore. In conclusion, while creatine has no immediate effect on sperm motility, it did increase the percentage of motile porcine sperm during …
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