Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Acinar cells"”.
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LINEAGE TRACING ANALYSIS OF PANCREATIC ACINAR CELL DE DIFFERENTIATION AND TRANS-DIFFERENTIATION IN VITRO AND IN VIVO IN THE ABSENCE OF MIST 1
Previous studies suggest that mature pancreatic acinar cells can trans- differentiate into duct and islet cells in vitro; however, the intrinsic factors regulating this process have not been defined. The acinar specific transcription factor MIST1 is required for acinar cell maturation and targeted …
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Investigating the role of the basic helix-loop-helix transcription factor MIST1 in pancreatic diseases
<p>Acinar cells of the exocrine pancreas are dedicated to synthesize, package and secrete immense quantities of pro-digestive enzymes to maintain proper metabolic homeostasis for the organism. Dysregulation of enzyme secretion in acinar cells can give rise to exocrine diseases including acute …
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Mechanism of exocrine pancreatic insufficiency during diabetes mellitus
… , respectively. Stimulation of fijra-2-AM loaded acinar cells with CCK-8 resulted in marked increases in peak [Ca2 ]a in both control (517.4 ± 12.09 nM) and diabetic rats (582 ± 16.05 nM) . The Ca2 transient composed of an initial peak followed by a plateau phase. The effects of CCK-8 in the …
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The Physiology and Pharmacology of FGF21 in the Exocrine Pancreas
… upon feeding. This function is mainly carried by acinar cells that require strict regulation of protein processing and stress-related pathways such as the integrated stress response pathway (ISR). Genetic, chemical or traumatic events can trigger an inflammatory response in the pancreas known as …
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The effects of ageing and streptozotocin (STZ) - induced diabetes mellitus on the rodent parotid gland
… with connective tissues, lipid droplets and mast cells compared to younger control glands. A significant (P<0.05) reduction in the mean acinar cell number was also observed in aged glands. Parotid glands taken from STZ-induced diabetic rats showed extensive infiltration of lipid droplets when …
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Influence of magnesium on salivary gland secretion: physiological and pathophysiological studies
… events in a number of epithelial secretory cells including the exocrine pancreas, lachrymal and the parietal cells. Since the salivary glands acinar cells have a similar structure and function as the exocrine pancreas, the stomach and the lachrymal gland, it was decided to investigate …
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The effect of ageing on the morphology and physiology of the lacrimal gland
… gland and in particular the lacrimal gland acinar cells. This study employed light and electron microscopy, immunohistochemical, radiobiological and physiological techniques to investigate ageing changes in the morphology and function of the lacrimal gland and acinar cells. In all …
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Cellular Mechanism of Exocrine Pancreatic Insufficiency in Diabetes Mellitus
… secretion or humanely killed and pancreatic acinar cells isolated for the measurement of intracellular free calcium and magnesium concentrations ([Ca 2+ ]1 and [Mg2+ ]1), total protein, and amylase output employing the Phadebas method. For rats in both in vivo and in vitro studies, fasting …
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Molecular mechanism of magnesium transport in epithelial cells
… secretion and Ca 2 homeostasis in the pancreatic acinar cells and parietal cells and (b) to characterise the molecular mechanism of Mg 2 transport using fluorimetric and spectroscopic studies. The results have shown that application of either cholecystokinin-octapeptide (CCK-8) or acetylcholine …
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Neuronal transmission mechanisms
… survey of the ultrastructure was made. The acinar cells were of two distinct types: peripheral and central cells. The ducts that these cells give rise to could be classified into three morphologically distinct areas. The fine structure of the reservoir ducts was also studied.
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Tumour suppression mediated through DPC4, p53 and APC
… mediator, on murine primary cultured pancreatic acinar cells. Previously p53 ApcM "i+ mice have been shown to develop pancreatic tumours. To examine the effect of Smad4 heterozygous mutation on the development of these tumours, Smad4 +i mutation was introduced into p534- and p531- ApcM "v+ mice. …
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Interactions of islet hormones with acetylcholine and cholecystokinin-octapeptide in the isolated pancreas of normal and diabetic rats
… stimulation of isolated pancreatic segments and acinar cells with different concentrations of either glucagon, somatostatin, VIP, galanin, ACh or CCK-8 resulted in different effects on amylase secretion and [Ca 2']i Combining the islet hormones with either ACh or CCK-8 resulted in marked …
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The Secretory Pathway Calcium ATPase 2C promotes increased cytosolic calcium levels
Pancreatic acinar cell exocytosis requires precise calcium (Ca2+) signals. When cytosolic Ca2+ levels remain high, cellular functions are disrupted, which is associated with initiation of pancreatitis. Ca2+ signals are achieved through regulating endoplasmic reticulum (ER) stores, Ca2+ATPases, and …
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RBP-L and RBP-J Have Critical Roles in the Functioning of Two Forms of the Pancreas Transcription Factor Complex PTF1
The decision of pancreatic precursor cells to differentiate into acinar or endocrine cells is regulated by a complex network of signaling and transcription factor pathways. P48 is a tissue-specific basic-helix-loop-helix (bHLH) transcription factor, which is essential for pancreas development and …
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Establishing The Role Of The Pancreatic Transcription Factor Mist1 In Xbp1-Mediated Maintenance Of Pancreatic Acinar Cell Homeostasis
<p>Pancreatic acinar cells (PACs) continuously produce more protein than any other cell type in the human body. As a result, PACs and other specialized secretory cells have a constant demand placed on their protein synthetic and packaging machinery. When demand for secreted products exceeds the …
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Comparison of Ecto-Nucleotidase Activity in PAR-C5 Cells and Native Rat Parotid Gland
Cells from a parotid gland acinar cell line (PAR-C5) cells were investigated to compare the activity of the ecto-nucleotidase enzyme in those cells to those of native parotid gland acinar cells. The object of this study was to determine how useful the PAR-C5 cells are as a model for salivary gland …
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Studies on the Secretory Process of Rabbit Parotid
… medium for 9 to 10 hr during which period their acinar cells retain their organization, synthesize proteins at a constant rate, and discharge their secretory proteins by exocytosis upon adrenergic stimulation. Autoradiographic experiments have shown that the acinar cells of the system transport …
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Elucidating the Role of Cellular Architecture in the Developing Pancreas
… the events necessary in order to generate these cells in vitro for replacement therapies. Directed differentiation protocols have improved upon transitioning from 2D to 3D cultures, indicating that the 3D microenvironment in which β cells are born is critical for the acquisition of their cell …
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Isoprenylation and NET formation in acute pancreatitis
… myeloperoxidase activity, chemokine levels, and acinar cell necrosis and edema formation in the pancreas. In paper I, farnesyltransferase mediates leukocyte sequestration and tissue injury in AP. Inhibition of farnesyltransferase attenuates infiltration of neutrophils in the pancreas and the lung …
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Molecular Cloning of the cDNA for Porcine Parotid Hormone
… staining of porcine tissues indicated that the acinar cells of the parotid glands are the primary location for both parotid hormone related mRNAs and the translation products.</p> <p>The expression of cloned parotid hormone containing cDNAs produced a single protein in the cell-free system and …
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