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Showing 1 to 7 of 7 for “"Acetyllysine"”.

  1. N⁶-formylation of lysine : a pathological secondary modification of proteins

    … similarity to the biologically important N⁶- acetyllysine has raised questions about its mechanisms of formation and biological consequences. Using novel ultrasensitive and specific liquid chromatography-coupled tandem mass spectrometry methods (LC-MS/MS) to quantify N⁶-formyllysine lesions in …

    mit Repository record for N⁶-formylation of lysine : a pathological secondary modification of proteins (opens in a new tab)

  2. Quantitative approaches to probe the acetylproteome

    … degenerate peptide arrays as well as develop acetyllysine affinity reagents as an alternative to anti-acetyllysine antibodies. Additionally, bioorthogonal proteomics were employed to identify acetyltransferase substrates. Taken together, the knowledge generated and the methods developed …

    mit Repository record for Quantitative approaches to probe the acetylproteome (opens in a new tab)

  3. Expanding the genetic code of a mouse

    … (Davis and Chin, 2012) In this study, I used Acetyllysine(AcK) as unnatural amino acids, N? -acetyl-lysyl-tRNA synthetase (AcKRS) as AcK-tRNA synthetase, pyrrolysyl-tRNA(PylT) as tRNA from Methanosarcina mazei (Mukai et al., 2008). Amber codon was inserted in GFP that is role of reporter gene. …

    ajou Repository record for Expanding the genetic code of a mouse (opens in a new tab)

  4. Mechanistic Enzymology Of Plasmodium Falciparum Sir2, Structural And Biochemical Studies On Nicotinamidases And The Structure And Function Of Enzymes In The Purine Degradative Pathway From Klebsiella Pneumoniae

    … shown to be an NAD+-dependent deacetylase and an acetyllysinedependent and acetyllysine-independent NAD+ glycohydrolase. In addition, several examples of the Sir2 modulating enzyme, nicotinamidase, are characterized both structurally and biochemically. These works provided additional insights into …

    cornell Repository record for Mechanistic Enzymology Of Plasmodium Falciparum Sir2, Structural And Biochemical Studies On Nicotinamidases And The Structure And Function Of Enzymes In The Purine Degradative Pathway From Klebsiella Pneumoniae (opens in a new tab)

  5. Role of P300 Zz Domain In Chromatin Association and Histone Acetylation

    … on histone H3K27 and H3K18, whereas the acetyllysine binding activity of BRD is required for acetylation of virtually all lysine residues in H3 and H4 tails.</p> <p>p300/CBP are frequently mutated or dysregulated in many human diseases including cancer. We found that p300 is overexpressed …

    uthsc Repository record for Role of P300 Zz Domain In Chromatin Association and Histone Acetylation (opens in a new tab)

  6. Control of Regulatory Element Function by Histone H3.3

    … promoters, along with reduced enrichment of the acetyllysine reader BRD4. Despite the enrichment of H3.3 at both promoters and enhancers, it appears to play distinct roles at these regions. ESCs lacking H3.3 or HIRA are able to maintain both accessibility and TF footprinting at enhancers, but …

    utswmed Repository record for Control of Regulatory Element Function by Histone H3.3 (opens in a new tab)