Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Acetyl-CoA Carboxylase (ACC)"”.
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Novel Metabolic Regulation of Invadopodia and Invasion by Acetyl-CoA Carboxylase and Lipogenesis
… and function of invadopodia. Inhibition of acetyl-CoA carboxylase (ACC), the rate-limiting step of fatty acid synthesis, reduced invadopodia incidence in Src-transformed 3T3 (3T3-Src) cells. Blocking ACC or fatty acid synthase (FASN) activity also altered pathway activity and cellular …
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Studies on the Regulation of Acetyl-CoA Carboxylase and its Influence on Bacillus subtilis Differentiation
In starving Bacillus subtilis cells, the accDA operon encoding two subunits of the essential enzyme acetyl-CoA carboxylase (ACC) is a direct target of the master regulator of sporulation, Spo0A. Binding of Spo0A to a cis-element (0A box) in the promoter region of the operon has recently been …
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Phosphorylation of Skeletal Muscle Acetyl-CoA Carboxylase by AMPK Enhances Palmitoyl-CoA Inhibition
Acetyl-CoA carboxylase (ACC) catalyzes the formation of malnoyl-CoA, which in turn controls the rate of fatty acid metabolism. ACC beta or 2 has been shown to be localized on the mitochondria in close proximity to carnintine palmitoyl transferase 1 (CPT-1), the enzyme responsible for the influx of …
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Investigating IL-15 Metabolic Impact and Its Mechanism of Action in Skeletal Muscle Cells
… troponin T expression and Akt, AMPK and Acetyl-CoA Carboxylase (ACC) phosphorylation state. Acute IL-15 treatment increased glucose uptake without activating insulin signaling pathway or GLUT4 translocation. Furthermore, acute IL-15 treatment increased resting oxygen consumption rate …
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NAD+ salvage by Nicotinamide phosphoribosyltransferase (Nampt) mediates lipogenesis, energy homeostasis, and survival in prostate cancer
… of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC). Inhibition of the NAD<super>+</super>-dependent sirtuin deacetylases by Sirtinol treatment, or SIRT1/SIRT3 knockdown, phenocopied the effects of Nampt blockade on fatty acid synthesis thereby suggesting that Nampt …
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Molecular regulation of the cardiac-enriched acetyl-CoA carboxylase isoform (ACCβ) : a novel target for therapeutic interventions in cardiovascular disease
… 1 (CPT1). CPT1 is potently inhibited by malonyl-CoA, the product of the acetylCoA carboxylation reaction that is catalyzed by acetyl-CoA carboxylase (ACC). Recent studies have demonstrated that metabolic fuels such as fatty acids and glucose can function as signaling ligands, directing …
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Metabolic Engineering of oleaginous yeast for the production of biofuels
… examined within the lipid synthesis pathway: acetyl-coA carboxylase (ACC), delta9-desaturase (D9), ATP citrate lyase (ACL), and diacylglycerol acyltransferase (DGA). A combinatorial investigation revealed the order of contribution to lipid overproduction (from strongest to weakest): DGA, ACC, …
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Involvement of AMP-activated protein kinase in differential regulation of appetite between lines of chickens selected for low or high juvenile body weight
… LWS chickens had higher levels of AMPK α and acetyl-CoA carboxylase (ACC) phosphorylation, which was caused by upregulation of the upstream factor calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK β). There was greater mRNA expression of carnitine palmitoyltransferase I (CPT1), …
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Identification of molecular targets regulating fatty acid synthesis in bovine mammary epithelial cells
… The regulation of lipogenic genes including acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) is controlled by transcription factors including sterol regulatory element binding protein-1 (SREBP1) and liver X receptor (LXR). In vivo, diet-induced milk fat depression or supplementing …
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Purification and characterization of an insulin-stimulated kinase
… kinases include key metabolic enzymes such as acetyl-CoA carboxylase (ACC) and the ribosomal S6. While, the S6 kinases have now been purified and characterized, very little is known about the nature of the proteinkinase(s) able to phosphorylate ACC particularly on the insulin-directed site. …
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Activity and mRNA abundance of enzymes for fatty acid synthesis and desaturation in mammary cell cultures
… (SA), oleic acid (OA), elaidic acid (EA), trans-vaccenic acid (TVA), linoleic acid (LA) or conjugated linoleic acid (CLA) were supplemented in the media of mouse mammary epithelial (MME) cells that were grown to confluence in Dulbecco's modified Eagle's medium (DMEM). As indicated by cellular …
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Studies of Escherichia coli Acetyl-CoA carboxylase
Acetyl-CoA carboxylase (ACC) initiates the first committed step of fatty acid synthesis. ACC is composed of three separate components: biotin carboxylase (BC), carboxyltransferase (CT), and biotin carboxyl carrier protein (BCCP). BC carries out the first half of the ACC reaction where it catalyzes …
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Metabolic engineering strategies for increasing lipid production in oleaginous yeast
… the simultaneous overexpression of the native acetyl-CoA carboxylase (ACC 1) and diacylglycerol acyltransferase (DGA2) genes; the resulting strain was used as a benchmark to evaluate our own efforts. We began with the compilation of a set of 44 genes and evaluated the effects of the individual …
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Stearoyl-COA Desaturase Gene Transcription, mRNA, And Activity In Response To Trans-Vaccenic Acid And Conjugated Linoleic Acid Isomers
… investigate: 1) desaturation of dietary trans-vaccenic acid (TVA, trans11-18:1) to the cis9,trans11-18:2 isomer of conjugated linoleic acid (9/11CLA), 2) effects of two conjugated linoleic acid isomers [9/11CLA or trans10,cis12-18:2 (10/12CLA)] and TVA on enzyme activities and mRNA abundance for …
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ROLE OF LYSOPHOSPHATIDIC ACID IN REGULATION OF CANCER CELL METABOLISM
… AMP-activated kinase (AMPK), thereby activating acetyl CoA carboxylase (ACC). Moreover, these effects of LPA were mediated by LPA2, a receptor subtype overexpressed in multiple cancers, providing an explanation for the cancer specific regulation of FAS and ACC by LPA. Downstream of the LPA2 …
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Molecular biology of the entomopathogenic fungus Beauveria bassiana : insect-cuticle degrading enzymes and development of a new selection marker for fungal transformation
… Soraphen A specifically inhibits the eukaryotic Acetyl-CoA Carboxylase (ACC) but not prokaryotic ACC. In fungi, three ACC functional domains are located on a multifunctional polypeptide derived from the expression of a single gene. In contrast, these three domains of E. coli ACC reside on four …
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The effect of metformin on vascular function and AMP-activated protein kinase activation in Type 2 diabetes
… insulin resistance, endothelial dysfunction and accelerated atherosclerosis, but the molecular mechanisms underlying this link are incompletely understood. Biguanides and thiazolidinediones are widely used in the treatment of Type 2 diabetes to improve glycaemia but the precise site, mode and …
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Cellular Mechanism of High Glucose/Palmitate-induced INS-1 Beta Cell Death
… islets. The cellular injury, through excess FFA accompanied by triglyceride accumulation, was termed beta cell glucolipotoxicity. The cellular mechanisms involved in FFA-induced beta cell apoptosis are not fully understood. FFAs are usually non-toxic to beta cells if they are oxidized. However, …
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Investigation of molecular regulatory control of the cardiac-enrich acetyl-CoA carboxlase
… The current study was centred on the enzyme acetyl-CoA carboxylase (ACC) which catalyses the carboxylation of acetylCoA to generate malonyl-CoA, a central metabolite in the regulation of fatty acid oxidation (FAO). There are two isoforms of ACC, ACCa. and ACCj3, both of which are present in …
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ROLES OF LIPOGENESIS IN BREAST CANCER PROGRESSION
… facilitating the condensation reaction between acetyl-CoA and malonyl-CoA in the presence of NADPH. We observed that normal breast epithelial cells MCF10A cells have very low level of FAS expression whereas breast cancer cell lines MCF7, MDA MB231 and MDA MB231 LM have significant …