Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 51 for “"Accessory protein"”.
-
Regulation of Mu transposition via communication between the transposase and Mu-encoded accessory protein
… the Mu transposase, MuA, and a Mu-encoded accessory protein, MuB. Together, these two proteins regulate Mu transposition and targeting. We demonstrate that MuB interacts with multiple subunits of the MuA transposase complex to stimulate transposition. These results corroborate previous …
-
Analyzing the Interaction Between Melanocortin 5 Receptor, Melanocortin Receptor Accessory Protein 1, and Melanocortin 2 Receptor in Rainbow Trout
… cell, depending upon the presence of other local proteins. The interaction of rtMC5R with MRAP1 and MC2R, and the implications of such interactions will be described in this thesis.</p>
-
Association of the Interleukin-1 beta and Interleukin-1 Receptor Accessory Protein Gene Polymorphisms with Persistent Hepatitis B Virus Infection and Hepatocellular Carcinoma
… and immune defense via IL-1 receptor accessory protein (IL-1RAcP). We investigated whether genetic polymorphisms of IL-1B and IL-1RAcP genes are associated with persistent HBV infection and the presence of hepatocellular carcinoma (HCC). Methods: We enrolled a total of 292 patients …
-
Evaluating the Interaction Between the Human Melanocortin-2 Receptor and the Accessory Protein, Mrap1: Chimeric Receptor and Alanine Substitution Studies on Transmembrane Domain 4, Extracellular Loop 2, and Transmembrane Domain 5
… The MC2R requires the melanocortin receptor accessory protein-1 (MRAP1) for proper trafficking and activation of the receptor by the melanocortin hormone, ACTH. MRAP1 is a single transmembrane-spanning domain protein that creates a homodimer with another MRAP1 protein. Furthermore, MRAP2 …
-
An expanded search for RNA-programmable genomic engineering effectors
… Whereas previous search strategies relied on protein proximity to CRISPR-associated spacer acquisition proteins Cas1 and Cas2, this approach was seeded on CRISPR arrays alone. What resulted was the discovery of a potential new Class 2 CRISPR system, with two subtypes as characterized by …
-
Amino Acid Residues Implicated in the Interaction of Melanocortin Ligands and Their Receptors: A Study of MC2R Selectivity
… hormone. With the recent discovery of the MC2R accessory protein (MRAP), creating a heterologous system is now feasible. Using a general cell line like CHO-K1 cells, which do not express endogenous MCRs, we were able to create a heterologous expression system and test the selectivity of MC2R …
-
Characterization of the Gifsy-1 Phage Excisionase
… host chromosome. The reaction requires and accessory protein, Integration Host Factor (IHF). This forward recombination reaction between phage encoded attP and host encoded attB is referred to as phage integration. Integration results in the creation of two attachment sites that border the …
-
The cellular and molecular basis of the Nef requirement for HIV-1 infectivity
Nef is an HIV -1 accessory protein with a fundamental role for virus replication in vivo and for the development of AIDS. Among its several activities, Nef is essential for full HIV-1 infectivity, a function highly prominent in lymphoid cells. So far, the mechanism by which Nef promotes HIV-1 …
-
Characterization of Genes Specifically Expressed in Embryonic and Cancer Stem Cells
… with the natural existence of OR expression accessory protein. This OR changed cell cycle indicating that OR acts as a regulatory member of cell cycle control system. This study is the clue to understand the basic mechanism of cell cycle control system of ES cells and cancer cells. Futhermore …
-
Insight into G Protein-Coupled Receptors through Molecular Dynamics Simulations
… significantly to the understanding of protein-drug recognition, providing detailed information on structural flexibility and conformational changes, the supervised molecular dynamics(SuMD) allows to understand the (un)binding paths differences, and metadynamics (MetaD) enhances the …
-
Analysis of factors that affect pseudotyping in retroviruses
… pseudotype with various envelope glycoproteins; however, the molecular mechanism of glycoprotein acquisition process is not completely understood even with their native Env proteins. Understanding this mechanism would aid development of a safe and efficient retroviral gene therapy …
-
Single-molecule studies of mechanical and helicase-catalyzed disruption of nucleic acid duplexes
… directly observing individual motor steps of the protein. Then, we examine the factors influencing the ability of UvrD to switch between unzipping and re-zipping behaviors. Finally, we place UvrD in its biological context by observing the effect of its interactions with an accessory protein in DNA …
-
Structural insight into the assembly of iron-sulfur clusters and their function in radical generation
… consistent with structures solved of homologous proteins. The length of this loop is thought to restrain the active site cysteine, interfering with its ability to affect catalysis. Our results are consistent with the theory that this cysteine desulfurase requires an accessory protein for fully …
-
HIV-1 Vpu Oligomerization in Membrane Mimetics
… into a manageable chronic condition. Among other proteins, HIV-1 encodes the Vpu accessory protein which upregulates the process of viral release from infected cells, providing an enticing target for future anti-HIV-1 therapies. In this thesis, I outline a conserved AxxAxxxAxxxA motif responsible …
-
Characterization of Reduced Phosphorus Metabolism in Alcaligenes Faecalis WM2072 and Xanthobacter Flavus WM2814
… designated htxZ, that encodes a homolog of an accessory protein for soluble NAD-dependent formate dehydrogenases. Experiments in the heterologous host Xanthobacter autotrophicus show that htxX and htxY are required for hypophosphite oxidation and ptxD is required for phosphite oxidation in this …
-
Further characterisation of the envelope stress responses of Salmonella Typhimurium
… transduction system and characterisation of its accessory protein, ZraP. We suggest ZraR mediated positive regulation of the virulence factors MntH and GroEL, GroSL, DnaK and ClpB (the heat shock chaperones). In addition, we show critical requirement of ZraSR for maximal carbon-starvation induced …
-
Innate sensing and regulation during enteric caliciviruses infections
… norovirus to subvert it through expression of an accessory protein is demonstrated. We show that both RIG-I and STING are required for a robust interferon response to infection with MNV1 in primary BMDMs and RAW264.7 cells, with a significant increase in viral titres following infection in RIG-I- …
-
Visualizing Protease Activation, Retrochmp3 Activity, and VPR Recruitment During HIV-1 Assembly
… virions, elucidated the mechanism by which the protein retroCHMP3 has an antiviral function, and examined the kinetics of recruitment of the HIV-1 accessory protein Vpr. I have also added significantly to science communication research through a study which evaluated the effectiveness of science …
-
Targeting protein: protein interaction sites for drug development against voltage-gated sodium channels
… of Nav channels is a source of less conserved protein-protein interaction (PPI) interfaces that represent a novel opportunity for designing isoform-specific chemical leads targeting Nav channels. The macromolecular complex of Nav channels is regulated by a number of accessory proteins. Very few …
Page 1 of 3