Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"ATPase domain"”.
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ROLES OF ACCESSORY DOMAINS IN THE CATALYTIC SUBUNIT OF ISW2
… The catalytic subunit of ISW2 consists of the ATPase domain and three domains at the C-terminus called HAND, SANT and SLIDE (HSS) domains. The individual roles of each HSS domains and how they coordinate with the ATPase domain during remodeling are not completely understood. Initial studies of …
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THE MECHANISMS OF ISW1A, ISW1B AND ISW2 CHROMATIN REMODELERS
… ISW1a, role of accessory subunits and various domains/motifs of ISWI complexes in nucleosome remodeling, and detailed remodeling mechanism of nucleosomes by ISW1a. ISW1a and ISW1b share the same catalytic subunit, but in vivo they are localized at different locations and is also known to have …
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Interaction of HspBP1 with Hsp70
… HspBP1 is an Hsp70 cochaperone that binds to the ATPase domain of Hsp70 and inhibits its ATPase activity. The purpose of these studies was to gain a basic understanding of the Hsp70/HspBP1 interaction. The first goal was to define the structural domains of HspBP1 and explore the secondary …
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STRUCTURAL AND FUNCTIONAL DELINEATION OF SUBUNITS AND DOMAINS IN THE SACCHAROMYCES CEREVISIAE SWI/SNF COMPLEX
… activities are fulfilled by a conserved ATPase domain in the catalytic subunit, there are accessory domains and subunits that are speculated to regulate these activities. Important questions in the field center around the identification of these domains and subunits, whether they affect …
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STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE ISW2 CHROMATIN REMODELING COMPLEX
… Isw2 subunit consists of several conserved domains. The highly conserved ATPase domain is present at the N-terminus whereas the conserved HAND, SANT and SLIDE domain are towards the carboxyl terminal end of Isw2. Nucleosome mobilization by ISW2 requires both extranucleosomal DNA and the …
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Investigating the function of the hereditary spastic paraplegia protein spastin in the endomembrane system
… using energy derived from ATP hydrolysis by its ATPase domain. The location of this activity is governed by spastin’s localisation domains which mediate recruitment to membrane sites including endosomes and the ER. In this thesis I aimed to elucidate the function of spastin at these sites, as …
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Assessing the role of Hsp70 in prion propagation in Saccharomyces cerevisiae
… conserved protein composed of an N-terminal ATPase domain, a peptide-binding domain (PBD) and a C-terminal domain. In this study we describe a genetic screen that identifies an array of mutants within different domains of the major cytosolic Hsp70 chaperones of yeast, Ssa1-4 which impair the …
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Design framework of the MuA remodeling signal that confers preferential complex disassembly by the AAA+ unfoldase ClpX
… non-native states. The Clp/Hsp100 family of ATPases are unfolding chaperones that remodel macromolecular complexes and facilitate ATP-dependent protein degradation. They are members of the superfamily of AAA+ enzymes (ATPases Associated with various cellular Activities), which is conserved …
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Redox Sensing By Yeast Hsp70 Facilitates Modulation of Protein Quality Control and The Cytoprotective Response
… by modification of two key cysteines in the ATPase domain by oxidizing or thiol-modifying compounds. To investigate the biological consequences of cysteine modification on the Hsp70 Ssa1 in budding yeast, I generated cysteine null (cysteine to serine) and oxidomimetic (cysteine to aspartic …
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Epic Search for the Perfect Partner: MutSgamma Promotes Efficient Meiotic Recombination and Homolog Pairing in Mouse Spermatocytes
… yeast and in mouse, that mutations to the ATPase domain of MSH5 have similar defects. These mutants do not form crossovers and have similar pairing and synapsis defects, but a key difference is that this mutant can still form the complex between MSH4 and MSH5. MutSgamma has been shown in …
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Staphylococcus aureus DNA gyrase: mechanism and drug targeting
… attributed to the differences in the Cterminal domain of the gyrase A-subunit (GyrA). The discovery of the two novel monovalent alkali metal cation (M+) binding sites located in N-terminal domain of GyrB by protein crystallography has suggested a novel role of these M+ ions in the supercoiling …
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MODES OF NUCLEOSOME INTERACTION AND MECHANISMS OF THE SACCHAROMYCES CEREVISIAE CHROMATIN REMODELERS INO80 AND ISW1A
… respectively. Despite sharing conserved domain architecture of the core remodeling machinery, chromatin remodelers differ significantly in their modes of interaction with nucleosomes, and how they alter histone-DNA contacts. In this study, we have used a site-specific photocrosslinking …
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Uncovering Reversible AMPylation of BiP Mediated by dFic During ER Homeostasis
… hydroxyl side chains of target substrates. Fic domain which mediates AMPylation is highly conserved across species, including higher eukaryotes, implicating an essential role of this modification in cellular function. Despite the recent discoveries and characterization of a number of bacterial …
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Uncovering Reversible AMPylation of BiP Mediated by dFic During ER Homeostasis
… hydroxyl side chains of target substrates. Fic domain which mediates AMPylation is highly conserved across species, including higher eukaryotes, implicating an essential role of this modification in cellular function. Despite the recent discoveries and characterization of a number of bacterial …
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The Roles of the Mcm2-7 Tails in Replication Initiation
… related subunits. Each subunit contains an AAA+ ATPase domain, a large OB-fold domain, and extensions of varying lengths on each terminus. Importantly, Mcm2, Mcm4, and Mcm6 contain long unstructured N-terminal tails, which are unrelated to each other and whose role in replication initiation is …
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Investigation of Interaction between heat shock protein 70.14 (Hsp 70.14) and RBBP6 RING finger domain
… is an Hsp70 variant that lacks the c-terminal domain but contains substrate binding domain and the ATPase domain. The postulated interaction of this protein with the RING finger domain of RBBP6, suggested their role in protein quality control system and chaperone-mediated ubiquitination. …
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Impact of the chromatin remodeller SMARCAD1 on murine intestinal intraepithelial lymphocyte and white adipose tissue biology.
… utilising an animal model in which the catalytic ATPase domain of murine SMARCAD1 had been deleted using Cre/lox technology. Preliminary results had implicated SMARCAD1 in adaptive-immunity and white adipose tissue biology, and SMARCAD1 expression in these tissues/cells was confirmed by …
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Functional Characterisation of a RECQL4 Mutation in Rothmund-Thomson Syndrome
… in the deletion of 66 amino acids in the RECQL4 ATPase domain. Despite the lower overall RECQL4 expression, single cell clones bearing the Mut-2 mutation showed mostly normal cell cycle distribution with a slight increase in population doubling times. When challenged with various DNA damaging …
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DISTINCT GENOME WIDE FUNCTIONS OF CHROMATIN REMODELERS IN NUCLEOSOME ORGANIZATION AND TRANSCRIPTION REGULATION
… into several families based on their conserved ATPase domain, an essential component required for their DNA translocation ability. In this study, we investigated the role yeast ISWI and SWI/SNF family of chromatin remodelers play on nucleosome rearrangement and transcription regulation by …
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Regulation of The Heat Shock Response By Thiol-Reactive Compounds In The Yeast Saccharomyces Cerevisiae
… Cysteine 303, located in the nucleotide-binding/ATPase domain of Ssa1, was shown to be modified <em>in vivo </em>by a model organic electrophile using Click chemistry technology, verifying that Ssa1 is a direct target for thiol-reactive compounds through adduct formation. Consistently, cadmium …
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